CD205 antibody | IL-A114
Filter by Application:
F ResetMouse anti Bovine CD205:RPE
- Product Type
- Monoclonal Antibody
- Clone
- IL-A114
- Isotype
- IgG1
- Specificity
- CD205
Antibody Quality and Performance Guarantee
| Mouse anti Bovine CD205 antibody, clone IL-A114 recognizes bovine CD205 (also known as DEC205) and originally described as BoWC6. CD205 is a cell surface antigen with an approximate molecular weight of ~210-220 kDa. CD205 is expressed on afferent lymph dendritic cells (veiled cells), B cells, a subset of T cells and by cortical and medullary thymocytes. |
- Target Species
- Bovine
- Species Cross-Reactivity
-
Target Species Cross Reactivity Sheep - N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG conjugated to R. Phycoerythrin (RPE) - lyophilized
- Reconstitution
- Reconstitute with 1.0 ml distilled water
Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. Bio-Rad recommend that the vial is gently mixed after reconstitution. - Preparation
- Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant.
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.09% Sodium Azide (NaN3)
1% Bovine Serum Albumin
5% Sucrose - Fusion Partners
- Spleen cells from immunized BALB/c mice were fused with cells of the X63.Ag8.653 myeloma cell line.
- Max Ex/Em
-
Fluorophore Excitation Max (nm) Emission Max (nm) RPE 488nm laser 496 578 - Regulatory
- For research purposes only
- Guarantee
- 12 months from date of despatch
Prior to reconstitution store at +4oC.
After reconstitution store at +4oC.
DO NOT FREEZE.
This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.
After reconstitution store at +4oC.
DO NOT FREEZE.
This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Flow Cytometry |  |
Neat |
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 1x106 cells in 100ul
How to Use the Spectraviewer
Watch the Tool Tutorial Video ▸- Start by selecting the application you are interested in, with the option to select an instrument from the drop down menu or create a customized instrument
- Select the fluorophores or fluorescent proteins you want to include in your panel to check compatibility
- Select the lasers and filters you wish to include
- Select combined or multi-laser view to visualize the spectra
| Description | Product Code | Applications | Pack Size | List Price | Your Price | Quantity | |
|---|---|---|---|---|---|---|---|
| Mouse IgG1 Negative Control:RPE | MCA928PE | F | 100 Tests |
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| Description | Mouse IgG1 Negative Control:RPE | ||||||
| Description | Product Code | Applications | Pack Size | List Price | Your Price | Quantity | |
|---|---|---|---|---|---|---|---|
| Bovine Dendritic Cell Growth Kit | PBP014KZZ | FN | 1 ml |
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| Description | Bovine Dendritic Cell Growth Kit | ||||||
| Bovine Dendritic Cell Growth Kit | PBP015KZZ | FN | 5 ml |
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| List Price | Your Price | ||||||
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| Description | Bovine Dendritic Cell Growth Kit | ||||||
References for CD205 antibody
-
Dutia, B.M. et al. (1993) Analysis of the monoclonal antibodies comprising WC6.
Vet Immunol Immunopathol. 39 (1-3): 193-9. -
Naessens, J. et al. (1993) Cross-reactivity of workshop antibodies with cells from domestic and wild ruminants.
Vet Immunol Immunopathol. 39 (1-3): 283-90. -
Gliddon, D.R. et al. (2004) DEC-205 expression on migrating dendritic cells in afferent lymph.
Immunology. 111 (3): 262-72. -
Parsons, K.R. et al. (1993) Studies of monoclonal antibodies identifying two novel bovine lymphocyte antigen differentiation clusters: workshop clusters (WC) 6 and 7.
Vet Immunol Immunopathol. 39 (1-3): 187-92. -
Howard, C.J. & Naessens, J. (1993) Summary of workshop findings for cattle (tables 1 and 2).
Vet Immunol Immunopathol. 39 (1-3): 25-47. -
Tamao, H. et al. (2011) Distribution of immune cells and expression of interleukin receptors in ileal Peyer's patches of calves.
Cell Tissue Res. 346: 245-54. -
Liu, J. et al. (2020) Theileria annulata. transformation altered cell surface molecules expression and endocytic function of monocyte-derived dendritic cells.
Ticks Tick Borne Dis. 11 (3): 101365.
- Synonyms
- WC6 Antigen
- UniProt
- Q6WY07
MCA2450PE
INN1111
INN1807
If you cannot find the batch/lot you are looking for please contact our technical support team for assistance.
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