CSF1R antibody | ROS-AV170
Cryostat sections were cut from 4 week old quail bursa of Fabricius. Clone ROS-AV170 specifically stains the bursal secretory dendritic cells (BSDC) located in the medulla and the macrophages of the interfollicular connective tissue. Image courtesy of Dr Nandor Nagy, Semmelweis University, Hungary
Clone ROS-AV170 specifically stains the bursal secretory dendritic cells (BSDC) located in the medulla. Clone ROS-AV170 also recognizes some CSF1R+ve macrophages in the interfollicular connective tissue. Image courtesy of Dr Nandor Nagy, Semmelweis University, Hungary
Cryostat sections we cut from 4 week old partridge bursa of Fabricius. Clone ROS-AV170 specifically recognized membrane expressed antigen on the bursal secretory dendritic cells (BSDC) in the medulla and by the macrophages (arrows) of the interfollicular connective tissue. Image courtesy of Dr Nandor Nagy, Semmelweis University, Hungary
Cryostat sections of 6 week old chicken bursa of Fabricius. A) Clone ROS-AV170 identifies strong membrane-bound immunoreaction on the cell surface of the bursal secretory dendritic cells (BSDC). B) Double immunofluorescence staining with Mouse anti vimentin, clone 3B4 (green) and Mouse anti Chicken CSF1R, clone ROS-AV170 (red), (product code MCA5956GA) shows that colocalization of vimentin with CSF1R occurs only in bursal dendritic cells. Image courtesy of Dr Nandor Nagy, Semmelweis University, Hungary
Mouse anti Chicken CSF1R:Alexa Fluor® 647
- Product Type
- Monoclonal Antibody
- Clone
- ROS-AV170
- Isotype
- IgG1
- Specificity
- CSF1R
Antibody Quality and Performance Guarantee
| Mouse anti Chicken CSF1R, clone ROS-AV170, recognizes the chicken homologue of human CSF1R, also known as CD115, CSF-1R, M-CSF receptor and c-fms. CSF1R is a single pass type 1 transmembrane protein belonging to the type III protein kinase family and is the cell surface receptor for both CSF1 and IL-34. Evidence from comparative sequence analysis across a range of vertebrate species indicates that CSF1 and IL-34 interact with distinct regions of CSF1R (Garceau et al. 2010). CSF1R cell surface expression appears to be one of the earliest events in macrophage lineage cells (Garceau et al. 2010).Mouse anti Chicken CSF1R, clone ROS-AV170 is a monoclonal antibody that has been generated to recognize chicken CSF1R . Clone ROS-AV170 has been shown to recognize the CSF1R antigen in some, but not all closely related avian species. Mouse anti Chicken CSF1R, clone ROS-AV170 is an excellent marker for chicken macrophages. |
- Target Species
- Chicken
- Species Cross-Reactivity
-
Target Species Cross Reactivity Goose Grey partridge Guinea Fowl Duck Quail Turkey Mouse Rat - N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG conjugated to Alexa Fluor® 647 - liquid
- Preparation
- Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.09% Sodium Azide (NaN3)
1% Bovine Serum Albumin - Immunogen
- Purified ChCSF1R-Fc fusion protein
- Approx. Protein Concentrations
- IgG concentration 0.05 mg/ml
- Fusion Partners
- Spleen cells from immunized Balb/c mice were fused with cells of the Sp2/0-Ag14 myeloma cell line
- Max Ex/Em
-
Fluorophore Excitation Max (nm) Emission Max (nm) Alexa Fluor®647 650 665 - Regulatory
- For research purposes only
- Guarantee
- 12 months from date of despatch
- Acknowledgements
- This product is provided under an intellectual property licence from Life Technologies Corporation. The transfer of this product is contingent on the buyer using the purchased product solely in research, excluding contract research or any fee for service research, and the buyer must not sell or otherwise transfer this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; (c) manufacturing or quality assurance or quality control, or (d) resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad CA 92008 USA or outlicensing@thermofisher.com
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Flow Cytometry |  |
Neat | 1/10 |
| Immunofluorescence | |
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 106 cells in 100ul
How to Use the Spectraviewer
Watch the Tool Tutorial Video ▸- Start by selecting the application you are interested in, with the option to select an instrument from the drop down menu or create a customized instrument
- Select the fluorophores or fluorescent proteins you want to include in your panel to check compatibility
- Select the lasers and filters you wish to include
- Select combined or multi-laser view to visualize the spectra
| Description | Product Code | Applications | Pack Size | List Price | Your Price | Quantity | |
|---|---|---|---|---|---|---|---|
| Human anti Chicken CD25:FITC | HCA173F | F | 0.1 mg |
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| Mouse anti Chicken Bu-1A:FITC | MCA2170F | F | 0.1 mg |
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| Description | Mouse anti Chicken Bu-1A:FITC | ||||||
| Mouse anti Chicken CD45:RPE | MCA2413PE | F | 100 Tests | Log in | |||
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| Description | Mouse anti Chicken CD45:RPE | ||||||
| Mouse anti Chicken Monocytes/Macrophages | MCA5770 | C F P * | 0.25 mg | Log in | |||
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| Description | Mouse anti Chicken Monocytes/Macrophages | ||||||
| Mouse anti Chicken CD14 | MCA5926GA | E WB | 0.1 mg |
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Source Reference
-
Garcia-Morales, C. et al. (2014) Production and characterisation of a monoclonal antibody that recognises the chicken CSF1 receptor and confirms that expression is restricted to macrophage-lineage cells.
Dev Comp Immunol 42:278-85.
References for CSF1R antibody
-
Garceau, V. et al. (2015) The development and maintenance of the mononuclear phagocyte system of the chick is controlled by signals from the macrophage colony-stimulating factor receptor.
BMC Biol. 13:12. -
Nagy, N. et al. (2016) Avian dendritic cells: Phenotype and ontogeny in lymphoid organs.
Dev Comp Immunol. 58: 47-59. -
Härtle, S. et al. (2024) Delineation of chicken immune markers in the era of omics and multicolor flow cytometry
Frontiers in Veterinary Science. 23 May [Epub ahead of print].
Further Reading
-
Garceau, V. et al. (2010) Pivotal Advance: Avian colony-stimulating factor 1 (CSF-1), interleukin-34 (IL-34), and CSF-1 receptor genes and gene products.
J Leukoc Biol. 87:753-64.
- Synonyms
- CD115
MCA5956A647
0614
153163
If you cannot find the batch/lot you are looking for please contact our technical support team for assistance.
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