CSF1R antibody | ROS-AV170

Mouse anti Chicken CSF1R:Alexa Fluor® 647

Product Type
Monoclonal Antibody
Clone
ROS-AV170
Isotype
IgG1
Specificity
CSF1R

Product Code Applications Pack Size List Price Your Price Qty
MCA5956A647
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SDS Safety Datasheet SDS
F IF 100 Tests/1ml loader
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Mouse anti Chicken CSF1R, clone ROS-AV170, recognizes the chicken homologue of human CSF1R, also known as CD115, CSF-1R, M-CSF receptor and c-fms. CSF1R is a single pass type 1 transmembrane protein belonging to the type III protein kinase family and is the cell surface receptor for both CSF1 and IL-34. Evidence from comparative sequence analysis across a range of vertebrate species indicates that CSF1 and IL-34 interact with distinct regions of CSF1R (Garceau et al. 2010).

CSF1R cell surface expression appears to be one of the earliest events in macrophage lineage cells (Garceau et al. 2010).Mouse anti Chicken CSF1R, clone ROS-AV170 is a monoclonal antibody that has been generated to recognize chicken CSF1R . Clone ROS-AV170 has been shown to recognize the CSF1R antigen in some, but not all closely related avian species.

Mouse anti Chicken CSF1R, clone ROS-AV170 is an excellent marker for chicken macrophages.

Target Species
Chicken
Species Cross-Reactivity
Target SpeciesCross Reactivity
Goose
Grey partridge
Guinea Fowl
Duck
Quail
Turkey
Mouse
Rat
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG conjugated to Alexa Fluor® 647 - liquid
Preparation
Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
1% Bovine Serum Albumin
Immunogen
Purified ChCSF1R-Fc fusion protein
Approx. Protein Concentrations
IgG concentration 0.05 mg/ml
Fusion Partners
Spleen cells from immunized Balb/c mice were fused with cells of the Sp2/0-Ag14 myeloma cell line
Max Ex/Em
Fluorophore Excitation Max (nm) Emission Max (nm)
Alexa Fluor®647 650 665
Regulatory
For research purposes only
Guarantee
12 months from date of despatch
Acknowledgements
This product is provided under an intellectual property licence from Life Technologies Corporation. The transfer of this product is contingent on the buyer using the purchased product solely in research, excluding contract research or any fee for service research, and the buyer must not sell or otherwise transfer this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; (c) manufacturing or quality assurance or quality control, or (d) resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad CA 92008 USA or outlicensing@thermofisher.com

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry Neat 1/10
Immunofluorescence
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 10ul of the suggested working dilution to label 106 cells in 100ul

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References for CSF1R antibody

  1. Garcia-Morales, C. et al. (2014) Production and characterisation of a monoclonal antibody that recognises the chicken CSF1 receptor and confirms that expression is restricted to macrophage-lineage cells.
    Dev Comp Immunol 42:278-85.
  2. Garceau, V. et al. (2015) The development and maintenance of the mononuclear phagocyte system of the chick is controlled by signals from the macrophage colony-stimulating factor receptor.
    BMC Biol. 13:12.
  3. Nagy, N. et al. (2016) Avian dendritic cells: Phenotype and ontogeny in lymphoid organs.
    Dev Comp Immunol. 58: 47-59.

Further Reading

  1. Garceau, V. et al. (2010) Pivotal Advance: Avian colony-stimulating factor 1 (CSF-1), interleukin-34 (IL-34), and CSF-1 receptor genes and gene products.
    J Leukoc Biol. 87:753-64.

Synonyms
CD115
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Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"

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