Burosumab antibody | AbD63872ia
Human anti Burosumab
- Product Type
- Monoclonal Antibody
- Clone
- AbD63872ia
- Isotype
- IgG1
- Specificity
- Burosumab
Antibody Quality and Performance Guarantee
HCA461
A microtiter plate was coated overnight with various antigens at a concentration of 5 μg/mL. After washing and blocking with PBST + 5% BSA, detection was performed using HRP conjugated Human Anti-Burosumab Antibody, clone AbD63872ia (HCA461) at a concentration of 2 μg/mL in HISPEC Assay Diluent (BUF049A) followed by QuantaBlu fluorogenic peroxidase substrate. HRP conjugation of the antibody used in this assay was performed using a LYNX Rapid HRP Antibody Conjugation Kit (LNK002P).
A microtiter plate was coated overnight with burosumab, recombinant His-tagged human FGF-23, Adalimumab, human IgG1 kappa control and human IgG1 lambda control at a concentration of 5 µg/mL. After washing and blocking with PBST + 5% BSA, detection was performed using HRP conjugated Human Anti-Burosumab Antibody, clone AbD63872ia (HCA461) titrated to the given concentrations in HISPEC Assay Diluent (BUF049A) followed by QuantaBlu Fluorogenic Peroxidase Substrate. Data for burosumab are shown as the mean of three measurements. HRP conjugation of the antibody used in this assay was performed using a LYNX Rapid HRP Antibody Conjugation Kit (LNK002P).
A microtiter plate was coated overnight with burosumab at a concentration of 1 µg/mL. After washing and blocking with PBST + 5% BSA, PBST with 10% human serum was added spiked with increasing concentrations of Human Anti-Burosumab Antibody, clone AbD63865ia (HCA459, black), clone AbD63870ia (HCA460, red), or clone AbD63872ia (HCA461, blue). Detection was performed using HRP conjugated burosumab at a concentration of 2μg/ml in HISPEC assay diluent (BUF049A) followed by QuantaBlu fluorogenic peroxidase substrate.
Data are shown as the mean of three measurements. HRP conjugation of the antibody used in this assay was performed using a LYNX Rapid HRP Antibody Conjugation Kit (LNK002P).
A microtiter plate was coated overnight with recombinant human FGF-23 at a concentration of 1 µg/mL. After washing and blocking with PBST + 5% BSA, a pre-incubated mixture of burosumab (0.15 µg/mL) plus increasing concentrations of Human Anti-Burosumab Antibody, clone AbD63872 (HCA461) in the monovalent Fab format was added. Free burosumab, still capable of binding to the human FGF-23-His coated plate, was detected using HRP conjugated Mouse Anti-Human IgG (Fc) CH2 Domain Antibody (MCA647P) followed by QuantaBlu Fluorogenic Peroxidase Substrate. Data are shown as the mean of three measurements. As a negative control, Human anti-Burosumab, clone AbD64797ad (TZA0111) was also tested.
Filter by Application:
E Reset| Human Anti-Burosumab Antibody, clone AbD63872ia is a paratope specific, inhibitory, anti-idiotypic antibody (Type 1) that specifically recognizes the free human monoclonal antibody drug burosumab. It does not recognize the drug target hFGF-23-His, nor burosumab in complex with hFGF-23-His. HCA461 is a fully human recombinant monoclonal antibody with IgG1 isotype and is suitable as a surrogate positive control or calibrator in an anti-drug antibody (ADA) assay. This antibody has a monovalent intrinsic affinity of 6.2nM and is one of the three antibodies offered for ADA assays. Antibody HCA459 has an affinity of 0.1nM and HCA460 has an affinity of 0.6nM. Burosumab (trade name Crysvita) is a humanized monoclonal antibody of the IgG1/kappa isotype that binds to fibroblast growth factor-23 (FGF23), blocking the interaction with the FGFR1. Burosumab therefore inhibits FGF23 signaling, thereby increasing tubular phosphate reabsorption and decreasing renal phosphate excretion, as well as increasing serum levels of 1,25(OH)2D and increasing GI absorption of phosphate. As a result, bone mineralization is improved and the risk of bone disease is decreased. Burosumab is indicated for the treatment of X-linked hypophosphatemia and tumor-induced osteomalacia. View a summary of all anti-burosumab antibodies |
- Product Form
- Human IgG1 antibody (lambda light chain) selected from the HuCAL phage display library and expressed in a human cell line. This antibody is supplied as a liquid.
- Preparation
- Purified IgG prepared by affinity chromatography on Protein A
- Source
- HKB-11
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.09% Sodium Azide (NaN3)
- Immunogen
- Burosumab
- Affinity
- The monovalent intrinsic affinity of Human anti-Burosumab Antibody, clone AbD63872ia was measured as KD = 6.2 nM by real time, label-free molecular interaction analysis on immobilized burosumab.
- Approx. Protein Concentrations
- Antibody concentration 0.5 mg/ml
- Regulatory
- For research purposes only
- Guarantee
- 12 months from date of despatch
- Acknowledgements
- This product and/or its use is covered by claims of U.S. patents, and/or pending U.S. and non-U.S. patent applications owned by or under license to Bio-Rad Laboratories, Inc. See bio-rad.com/en-us/trademarks for details.
Crysvita is a trademark of Kyowa Hakko Kirin and Ultragenyx Pharmaceutical. - Licensed Use
- For in vitro. research purposes and for commercial applications for the provision of in vitro testing services to support preclinical and clinical drug development. Any re-sale in any form or any other commercial application needs a written agreement with Bio-Rad.
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| ELISA |  |
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
- Technical Advice
- Recommended protocols and further information about HuCAL recombinant antibody technology can be found in the HuCAL Antibodies Technical Manual.
- ELISA
- Clone AbD63872ia can be used in indirect ELISA to detect burosumab. It can also be used as a surrogate positive control or a calibrator in an anti-drug antibody (ADA) assay, together with HCA459 and HCA460.
| Description | Product Code | Applications | Pack Size | List Price | Your Price | Quantity | |
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| Hispec Assay Diluent | BUF049A | E IY | 50 ml | Log in | |||
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| Description | Hispec Assay Diluent | ||||||
| Human anti Burosumab | HCA459 | E | 0.1 mg |
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| Description | Human anti Burosumab | ||||||
| Human anti Burosumab | HCA460 | E | 0.1 mg |
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| Description | Human anti Burosumab | ||||||
| Human anti Burosumab | TZA0109 | E | 0.1 mg |
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| Description | Human anti Burosumab | ||||||
| Human anti Burosumab | TZA0110 | E | 0.1 mg |
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| Description | Human anti Burosumab | ||||||
| Human anti Burosumab:HRP | TZA0110P | E | 0.1 mg |
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| Description | Human anti Burosumab:HRP | ||||||
| Human anti Burosumab (Drug/Target Complex) | TZA0111 | E | 0.1 mg |
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| Description | Human anti Burosumab (Drug/Target Complex) | ||||||
| Human anti Burosumab (Drug/Target Complex):HRP | TZA0111P | E | 0.1 mg |
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| Description | Human anti Burosumab (Drug/Target Complex):HRP | ||||||
- Synonyms
- Crysvita
View more products with Burosumab specificity
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