Burosumab antibody | AbD63869ad
Human anti Burosumab
- Product Type
- Monoclonal Antibody
- Clone
- AbD63869ad
- Isotype
- Fab antibody
- Specificity
- Burosumab
Antibody Quality and Performance Guarantee
A microtiter plate was coated overnight with various antigens at a concentration of 5 μg/ml. After washing and blocking with PBST + 5% BSA, detection was performed using Human Anti-Burosumab Antibody, clone AbD63869ad (TZA0109) at a concentration of 2 μg/ml in PBST followed by an HRP labelled anti-DYKDDDDK tag antibody in HISPEC Assay Diluent (BUF049A) and QuantaBlu fluorogenic peroxidase substrate.
A microtiter plate was coated overnight with Burosumab, recombinant His-tagged human FGF-23, Adalimumab, human IgG1 kappa control and human IgG1 lambda control at a concentration of 5 µg/ml. After washing and blocking with PBST + 5% BSA, detection was performed using Human Anti-Burosumab Antibody, clone AbD63869ad (TZA0109) titrated to the given concentrations in PBST followed by an HRP conjugated Anti-DYKDDDDK tag Antibody in HISPEC diluent (BUF049A) and QuantaBlu Fluorogenic Peroxidase Substrate. Data for burosumab are shown as the mean of three measurements.
A microtiter plate was coated overnight with recombinant His-tagged human FGF-23 at a concentration of 1 µg/ml. After washing and blocking with PBST + 5% BSA, a pre-incubated mixture of burosumab (0.15 µg/ml) plus increasing concentrations of Human Anti-Burosumab Antibody, clone AbD63869ad (TZA0109) was added. Free burosumab, still capable of binding to the human FGF-23-His coated plate was detected using HRP conjugated Mouse Anti-Human IgG (Fc) CH2 Domain Antibody (MCA647P) followed by QuantaBlu Fluorogenic Peroxidase Substrate. The non-inhibitory Human Anti-Burosumab Antibody, clone AbD64797ad (TZA0111) was used as a negative control. Data are shown as the mean of three measurements.
A microtiter plate was coated overnight with Human Anti-Burosumab Antibody, clone AbD63869ad (TZA0109) at a concentration of 1 µg/ml. After washing and blocking with PBST + 5% BSA, 10% human serum was added spiked with increasing concentrations of burosumab. Detection was performed using HRP conjugated Human Anti-Burosumab Antibody, clone AbD63865pap (TZA0110P) at a concentration of 0.5 µg/mL in HISPEC Assay Diluent (BUF049A) and QuantaBlu fluorogenic peroxidase substrate. Data are shown as the mean of three measurements.
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E Reset| Human Anti-Burosumab Antibody, clone AbD63869ad is a paratope specific, inhibitory anti-idiotypic antibody (Type 1) that specifically recognizes the humanized monoclonal antibody drug burosumab. It does not recognize the drug target hFGF-23-His, nor burosumab in complex with hFGF-23-His. This antibody can be used to measure free burosumab levels in serum from patients. A pair of anti-burosumab antibodies can be used to develop a pharmacokinetic (PK) bridging assay to measure free drug; clone AbD63869ad is recommended as a capture antibody, paired with Human Anti-Burosumab Antibody, clone AbD63865pap (TZA0110P) as the detection antibody. TZA0109 is a monovalent Fab antibody incorporating a SpyTag at the C-terminus end of the antibody heavy chain for conversion by the end user into alternative formats using any of the SpyCatchers available in our catalog. Burosumab (trade name Crysvita) is a humanized monoclonal antibody of the IgG1/kappa isotype that binds to fibroblast growth factor-23 (FGF23), blocking the interaction with the FGFR1. Burosumab therefore inhibits FGF23 signaling, thereby increasing tubular phosphate reabsorption and decreasing renal phosphate excretion, as well as increasing serum levels of 1,25(OH)2D and increasing GI absorption of phosphate. As a result, bone mineralization is improved and the risk of bone disease is decreased. Burosumab is indicated for the treatment of X-linked hypophosphatemia and tumor-induced osteomalacia. View a summary of all anti-burosumab antibodies |
- Product Form
- A monovalent human recombinant Fab (lambda light chain) selected from the HuCAL® phage display library, expressed in a proprietary E. coli. strain. The antibody is tagged with a DYKDDDDK tag, a SpyTag version 2 (VPTIVMVDAYKRYK) and a His6-tag (HHHHHH) at the C-terminus of the antibody heavy chain. This antibody is supplied as a liquid.
- Preparation
- Metal chelate affinity chromatography
- Source
- E.coli
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.0095% MIT
- Immunogen
- Burosumab
- Affinity
- The monovalent intrinsic affinity of AbD63869ad was measured as KD = 0.4 nM by real time, label free molecular interaction analysis on immobilized burosumab.
- Approx. Protein Concentrations
- Antibody concentration 0.5 mg/ml
- Regulatory
- For research purposes only
- Guarantee
- 12 months from date of despatch
- Acknowledgements
- This product and/or its use is covered by claims of U.S. patents, and/or pending U.S. and non-U.S. patent applications owned by or under license to Bio-Rad Laboratories, Inc. See bio-rad.com/en-us/trademarks for details.
His-tag is a registered trademark of EMD Biosciences.
Crysvita is a trademark of Kyowa Hakko Kirin and Ultragenyx Pharmaceutical. - Licensed Use
- For in vitro research purposes and for commercial applications for the provision of in vitro testing services to support preclinical and clinical drug development. Any re-sale in any form or any other commercial application needs a written agreement with Bio-Rad.
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| ELISA |  |
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
- Technical Advice
- Recommended protocols for coupling a Fab antibody with a SpyTag to a SpyCatcher can be found at SpyTag and SpyCatcher Products, and further information about HuCAL recombinant antibody technology can be found in the HuCAL Antibodies Technical Manual
- ELISA
- Clone AbD63869ad can be used in indirect ELISA to detect burosumab. It can also be used as a capture antibody to develop a pharmacokinetic (PK) bridging ELISA for burosumab together with TZA0110 or TZA0110P as the detection reagent.
Protocol: PK bridging ELISA
| Description | Product Code | Applications | Pack Size | List Price | Your Price | Quantity | |
|---|---|---|---|---|---|---|---|
| SpyTag3 Peptide | BLP086 | IY | 1 mg |
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| Description | SpyTag3 Peptide | ||||||
| Hispec Assay Diluent | BUF049A | E IY | 50 ml | Log in | |||
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| Description | Hispec Assay Diluent | ||||||
| Human anti Burosumab | HCA459 | E | 0.1 mg |
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| Description | Human anti Burosumab | ||||||
| Human anti Burosumab | HCA460 | E | 0.1 mg |
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| Description | Human anti Burosumab | ||||||
| Human anti Burosumab | HCA461 | E | 0.1 mg |
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| Description | Human anti Burosumab | ||||||
| LYNX Rapid HRP Antibody Conjugation Kit | LNK002P | CJ | 3 Conjugations For 400µg Antibody | Log in | |||
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| Description | LYNX Rapid HRP Antibody Conjugation Kit | ||||||
| Human anti Burosumab | TZA0110 | E | 0.1 mg |
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| Description | Human anti Burosumab | ||||||
| Human anti Burosumab:HRP | TZA0110P | E | 0.1 mg |
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| Description | Human anti Burosumab:HRP | ||||||
| Human anti Burosumab (Drug/Target Complex) | TZA0111 | E | 0.1 mg |
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| Description | Human anti Burosumab (Drug/Target Complex) | ||||||
| Human anti Burosumab (Drug/Target Complex):HRP | TZA0111P | E | 0.1 mg |
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| Description | Human anti Burosumab (Drug/Target Complex):HRP | ||||||
- Synonyms
- Crysvita
TZA0109
171449
If you cannot find the batch/lot you are looking for please contact our technical support team for assistance.
View more products with Burosumab specificity
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