Brentuximab Vedotin antibody | AbD39649ca
A microtiter plate was coated overnight with various antigens at a concentration of 5 μg/ml. After washing and blocking with PBST + 5% BSA, Human Anti-Brentuximab vedotin Antibody, clone AbD39649ca (HCA349) was added at a concentration of 2 μg/ml. Detection was performed using an alkaline phosphatase (AP) conjugated anti-DYKDDDDK tag antibody in HISPEC Assay Diluent (BUF049A) and AttoPhos Fluorogenic AP Substrate.
A microtiter plate was coated overnight with brentuximab vedotin, Human Anti-CD30 Antibody (Brentuximab Biosimilar, MCA6149), cetuximab, human IgG1 kappa or human IgG1 lambda at a concentration of 5 µg/ml. After washing and blocking with PBST + 5% BSA, Human Anti-Brentuximab vedotin Antibody, clone AbD39649ca (HCA349) titrated to the given concentrations in PBST was added. Detection was performed using Mouse Anti-Penta Histidine Tag:HRP Antibody (MCA5995P) in HISPEC Assay Diluent (BUF049A) and QuantaBlu Fluorogenic Peroxidase Substrate. Data for brentuximab vedotin and brentuximab are shown as the mean of three measurements.
A microtiter plate was coated overnight with Human Anti-Brentuximab vedotin Antibody, clone AbD39649ca (HCA349) at a concentration of 1 µg/ml. After washing and blocking with PBST + 5% BSA, 10% human serum was added spiked with increasing concentrations of brentuximab vedotin. Detection was performed using HRP conjugated Human Anti-Brentuximab vedotin Antibody, clone AbD39655ia (HCA351) at a concentration of 2 µg/ml in HISPEC Assay Diluent (BUF049A) and QuantaBlu Fluorogenic Peroxidase Substrate. HRP conjugation of the antibody used in this assay was performed using a LYNX Rapid HRP Antibody Conjugation Kit (LNK002P). Data are shown as the mean of three measurements.
A microtiter plate was coated overnight with recombinant human CD30 at a concentration of 1 µg/ml. After washing and blocking with PBST + 5% BSA, a pre-incubated mixture of brentuximab vedotin (0.15 µg/ml) plus increasing concentrations of Human Anti-Brentuximab vedotin Antibody, clone AbD39649ca (HCA349) in the monovalent Fab format was added. Free brentuximab vedotin, still capable of binding to the human CD30 coated plate, was detected using Mouse Anti-Human IgG (Fc) CH2 Domain:HRP Antibody (MCA647P) followed by QuantaBlu Fluorogenic Peroxidase Substrate. Data are shown as the mean of three measurements.
Human anti Brentuximab Vedotin
- Product Type
- Monoclonal Antibody
- Clone
- AbD39649ca
- Isotype
- HuCAL Fab monovalent
- Specificity
- Brentuximab Vedotin
Antibody Quality and Performance Guarantee
| Human Anti-Brentuximab vedotin Antibody, clone AbD39649ca is a paratope specific, inhibitory anti-idiotypic antibody that specifically recognizes the monoclonal antibody drug brentuximab, both alone and as the antibody drug conjugate (ADC), brentuximab vedotin. A pair of antibodies can be used to develop a pharmacokinetic (PK) bridging assay to measure free drug. This antibody, in monovalent Fab format, is recommended as the capture antibody, paired with Anti-Brentuximab vedotin Antibody in full immunoglobulin format, clone AbD39655ia (HCA351) as the detection antibody. Brentuximab vedotin (Adcetris) is a chimeric mouse/human IgG1 kappa ADC, approved by the FDA for the treatment of Hodgkin’s lymphoma (HL) and systemic anaplastic large cell lymphoma (ALCL). The brentuximab antibody selectively targets tumor cells expressing the cell membrane protein CD30, and delivers the conjugated toxin, monomethyl auristatin E (MMAE). Once inside the cell the MMAE is released from the antibody by proteolytic cleavage and disrupts the microtubule network inducing cell cycle arrest and apoptosis of the malignant cells, thereby preventing tumor growth and proliferation. View a summary of all brentuximab vedotin antibodies. |
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- Product Form
- A monovalent human recombinant Fab (kappa light chain) selected from the HuCAL phage display library, expressed in E. coli. The antibody is tagged with a DYKDDDDK tag and a HIS-tag (HHHHHH) at the C-terminus of the antibody heavy chain. This antibody is supplied as a liquid.
- Preparation
- Metal chelate affinity chromatography
- Source
- E.coli
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.09% Sodium Azide (NaN3)
- Immunogen
- Brentuximab vedotin
- Affinity
- The intrinsic affinity of the monovalent form of Human anti Brentuximab Vedotin antibody, clone AbD39649ca is KD = 1.3 nM as measured by real time, label free molecular interaction analysis on immobilized Brentuximab Vedotin.
- Approx. Protein Concentrations
- Antibody concentration 0.5 mg/ml
- Regulatory
- For research purposes only
- Guarantee
- 12 months from date of despatch
- Acknowledgements
- This product and/or its use is covered by claims of U.S. patents, and/or pending U.S. and non-U.S. patent applications owned by or under license to Bio-Rad Laboratories, Inc. See bio-rad.com/en-us/trademarks for details.
His-tag is a trademark of EMD Biosciences.
Adcetris is a trademark of Seattle Genetics. - Licensed Use
- For in vitro research purposes and for commercial applications for the provision of in vitro testing services to support preclinical and clinical drug development. Any re-sale in any form or any other commercial application needs a written agreement with Bio-Rad.
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| ELISA |  |
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
- Technical Advice
- Recommended protocols and further information about HuCAL recombinant antibody technology can be found in the HuCAL Antibodies Technical Manual.
- ELISA
- Human anti Brentuximab Vedotin antibody, clone AbD39649ca can be used as capture antibody for Brentuximab Vedotin in a bridging ELISA together with HCA351 as the detection reagent.
Protocol: PK bridging ELISA.
| Description | Product Code | Applications | Pack Size | List Price | Your Price | Quantity | |
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| Hispec Assay Diluent | BUF049A | E IY | 50 ml | Log in | |||
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| Description | Hispec Assay Diluent | ||||||
| Human anti Brentuximab Vedotin | HCA350 | E | 0.1 mg |
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| Description | Human anti Brentuximab Vedotin | ||||||
| Human anti Brentuximab Vedotin | HCA351 | E | 0.1 mg |
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| Description | Human anti Brentuximab Vedotin | ||||||
| Human anti Brentuximab Vedotin | HCA352 | E | 0.1 mg |
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| Description | Human anti Brentuximab Vedotin | ||||||
| LYNX Rapid HRP Antibody Conjugation Kit | LNK002P | CJ | 3 Conjugations For 400µg Antibody | Log in | |||
| List Price | Your Price | ||||||
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| Description | LYNX Rapid HRP Antibody Conjugation Kit | ||||||
| Mouse anti Penta Histidine Tag:HRP | MCA5995P | E WB | 125 µl |
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| List Price | Your Price | ||||||
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| Description | Mouse anti Penta Histidine Tag:HRP | ||||||
- Synonyms
- Adcetris
HCA349
161479
If you cannot find the batch/lot you are looking for please contact our technical support team for assistance.
View more products with BRENTUXIMAB VEDOTIN specificity
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