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Brentuximab Vedotin antibody | AbD39649ca

Human anti Brentuximab Vedotin

Product Type
Monoclonal Antibody
HuCAL Fab monovalent
Brentuximab Vedotin

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Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
E 0.1 mg loader
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Human Anti-Brentuximab vedotin Antibody, clone AbD39649ca is a paratope specific, inhibitory anti-idiotypic antibody that specifically recognizes the monoclonal antibody drug brentuximab, both alone and as the antibody drug conjugate (ADC), brentuximab vedotin.

A pair of antibodies can be used to develop a pharmacokinetic (PK) bridging assay to measure free drug. This antibody, in monovalent Fab format, is recommended as the capture antibody, paired with Anti-Brentuximab vedotin Antibody in full immunoglobulin format, clone AbD39655ia (HCA351) as the detection antibody.

Brentuximab vedotin (Adcetris) is a chimeric mouse/human IgG1 kappa ADC, approved by the FDA for the treatment of Hodgkin’s lymphoma (HL) and systemic anaplastic large cell lymphoma (ALCL). The brentuximab antibody selectively targets tumor cells expressing the cell membrane protein CD30, and delivers the conjugated toxin, monomethyl auristatin E (MMAE). Once inside the cell the MMAE is released from the antibody by proteolytic cleavage and disrupts the microtubule network inducing cell cycle arrest and apoptosis of the malignant cells, thereby preventing tumor growth and proliferation.

View a summary of all brentuximab vedotin antibodies

Product Form
A monovalent human recombinant Fab (kappa light chain) selected from the HuCAL phage display library, expressed in E. coli. The antibody is tagged with a DYKDDDDK tag and a HIS-tag (HHHHHH) at the C-terminus of the antibody heavy chain. This antibody is supplied as a liquid.
Metal chelate affinity chromatography
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
Brentuximab vedotin
The intrinsic affinity of the monovalent form of Human anti Brentuximab Vedotin antibody, clone AbD39649ca is KD = 1.3 nM as measured by real time, label free molecular interaction analysis on immobilized Brentuximab Vedotin.
Approx. Protein Concentrations
Antibody concentration 0.5 mg/ml
For research purposes only
12 months from date of despatch
This product and/or its use is covered by claims of U.S. patents, and/or pending U.S. and non-U.S. patent applications owned by or under license to Bio-Rad Laboratories, Inc. See for details.
His-tag is a trademark of EMD Biosciences.
Adcetris is a trademark of Seattle Genetics.

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Technical Advice
Recommended protocols and further information about HuCAL recombinant antibody technology can be found in the HuCAL Antibodies Technical Manual.
Human anti Brentuximab Vedotin antibody, clone AbD39649ca can be used as capture antibody for Brentuximab Vedotin in a bridging ELISA together with HCA351 as the detection reagent.
Protocol: PK bridging ELISA.

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Hispec Assay Diluent BUF049A E IY 50 ml
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Description Hispec Assay Diluent
Human anti Brentuximab Vedotin HCA350 E 0.1 mg
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Description Human anti Brentuximab Vedotin
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Description Human anti Brentuximab Vedotin
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Description Human anti Brentuximab Vedotin
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Licensed Use
For in vitro research purposes and for commercial applications for the provision of in vitro testing services to support preclinical and clinical drug development. Any re-sale in any form or any other commercial application needs a written agreement with Bio-Rad.



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Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"

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