Bevacizumab antibody | AbD17976_hIgG1
A microtiter plate was coated overnight with various antigens at a concentration of 5 μg/ml. After washing and blocking with PBST+5% BSA, HRP conjugated Human Anti-Bevacizumab Antibody, clone AbD17976_hIgG1 (HCA185) was added at a concentration of 2 μg/ml. Detection was performed using QuantaBlu Fluorogenic Peroxidase Substrate.
A microtiter plate was coated overnight with bevacizumab at a concentration of 1 µg/ml. After washing and blocking with PBST+5% BSA, Human Anti-Bevacizumab Antibody clone AbD17976_hIgG1 (HCA185) in PBST + 10% Human Serum was added. Detection was performed using HRP conjugated bevacizumab at a concentration of 2 µg/ml in HISPEC Assay Diluent (BUF049A) and QuantaBlu Fluorogenic Peroxidase Substrate.
A microtiter plate was coated over night with ranibizumab at a concentration of 1 μg/ml. After washing and blocking with PBST+5% BSA, 10% human serum was added spiked with increasing concentrations of Human Anti-Bevacizumab Antibody, clone AbD17976_hIgG1 (HCA185). Detection was performed using HRP conjugated ranibizumab at a concentration of 2 μg/ml in HISPEC Assay Diluent (BUF049A) followed by QuantaBlu Fluorogenic Peroxidase Substrate. HRP conjugation of the ranibizumab used in this assay was performed using a LYNX Rapid HRP Conjugation Kit (LNK002P). Data are shown as the mean of three measurements.
Human anti Bevacizumab
Anti-Bevacizumab Antibody is a recombinant, inhibitory, anti-idiotypic antibody in fully human immunoglobulin format; it is suitable as a positive control or calibrator in an immunogenicity assay to measure ADA levels for bevacizumab or biosimilars.
- Product Type
- Monoclonal Antibody
- Clone
- AbD17976_hIgG1
- Isotype
- IgG1
| Product Code | Applications | Pack Size | List Price | Quantity |
|---|---|---|---|---|
| HCA185 | E | 0.1 mg |
|
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| HCA185G | E | 1 mg |
|
Human Anti-Bevacizumab Antibody, clone AbD17976_hIgG1 is an affinity-matured, inhibitory, anti-idiotypic antibody that recognizes the humanized monoclonal antibody drugs bevacizumab and ranibizumab, and their biosimilar products.
Clone AbD17976_hIgG1 is a fully human recombinant monoclonal antibody with IgG1 isotype and is suitable as a positive control or calibrator in an anti-drug antibody (ADA) assay.
This Anti-Bevacizumab Antibody cross-reacts with the drug ranibizumab (Lucentis). Ranibizumab is a humanized Fab fragment antibody (VH3-23/kappa1), originating from the same parental mouse antibody as bevacizumab; it has 4 amino acid exchanges in the heavy chain and 1 amino acid exchange in the light chain compared to bevacizumab.
Bevacizumab (trade name Avastin) is a humanized monoclonal antibody (IgG1/kappa) that inhibits angiogenesis by binding to vascular endothelial growth factor alpha (VEGF-A). VEGF-A is a potent stimulator of angiogenesis of both normal and cancerous cells, and acts as a regulator of vasculogenesis. Bevacizumab is used to treat various cancers, including colorectal, lung, breast, glioblastoma, kidney and ovarian.
View a summary of all anti-bevacizumab antibodies.
Clone AbD17976_hIgG1 is a fully human recombinant monoclonal antibody with IgG1 isotype and is suitable as a positive control or calibrator in an anti-drug antibody (ADA) assay.
This Anti-Bevacizumab Antibody cross-reacts with the drug ranibizumab (Lucentis). Ranibizumab is a humanized Fab fragment antibody (VH3-23/kappa1), originating from the same parental mouse antibody as bevacizumab; it has 4 amino acid exchanges in the heavy chain and 1 amino acid exchange in the light chain compared to bevacizumab.
Bevacizumab (trade name Avastin) is a humanized monoclonal antibody (IgG1/kappa) that inhibits angiogenesis by binding to vascular endothelial growth factor alpha (VEGF-A). VEGF-A is a potent stimulator of angiogenesis of both normal and cancerous cells, and acts as a regulator of vasculogenesis. Bevacizumab is used to treat various cancers, including colorectal, lung, breast, glioblastoma, kidney and ovarian.
View a summary of all anti-bevacizumab antibodies.
Product Details
- Product Form
- Human IgG1 antibody selected from the HuCAL® phage display library and expressed in a human cell line. This antibody is supplied as a liquid.
- Preparation
- Purified IgG prepared by affinity chromatography on Protein A
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.01% Thiomersal
- Immunogen
- Bevacizumab
- Affinity
- The monovalent intrinsic affinity of this antibody was measured as KD=0.4 nM by real time, label-free molecular interaction analysis on immobilized bevacizumab.
- Approx. Protein Concentrations
- Pack Size: 0.1 mgIgG concentration 1.0 mg/mlPack Size: 1 mgTotal protein concentration 1.0 mg/ml
Storage Information
- Storage
- This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. - Guarantee
- 12 months from date of despatch
More Information
- Acknowledgements
- This product and/or its use is covered by claims of U.S. patents, and/or pending U.S. and non-U.S. patent applications owned by or under license to Bio-Rad Laboratories, Inc. See bio-rad.com/en-us/trademarks for details.
Avastin is a trademark of Genentech Inc. - Licensed Use
- For in vitro research purposes and for commercial applications for the provision of in vitro testing services to support preclinical and clinical drug development. Any re-sale in any form or any other commercial application needs a written agreement with Bio-Rad.
- Regulatory
- For research purposes only
Applications of Bevacizumab antibody
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| ELISA |
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
- Technical Advice
- Recommended protocols and further information about HuCAL recombinant antibody technology can be found in the HuCAL Antibodies Technical Manual
- ELISA
- This product is suitable for use in direct ELISA applications.
This antibody is fully human and can be used as a reference standard in an ADA assay.
Protocol: ADA bridging ELISA
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Useful Reagents Available
| Description | Product Code | Applications | Pack Size | List Price | Quantity |
|---|---|---|---|---|---|
| Human anti Bevacizumab | HCA182 | E | 0.1 mg |
|
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| Human anti Bevacizumab | HCA184 | E | 0.1 mg |
|
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| LYNX Rapid HRP Antibody Conjugation Kit | LNK001P | CJ | 1 Conjugation For 400µg Antibody | ||
| LYNX Rapid HRP Antibody Conjugation Kit | LNK002P | CJ | 3 Conjugations For 400µg Antibody |
Product Specific References
References for Bevacizumab antibody
-
Iwamoto, N. et al. (2018) Antibody drug quantitation in coexistence with anti-drug antibodies on nSMOL bioanalysis.
Anal Biochem. 540-541: 30-7.
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