Benralizumab antibody | AbD60095ad
Human anti Benralizumab
- Product Type
- Monoclonal Antibody
- Clone
- AbD60095ad
- Isotype
- Fab antibody
- Specificity
- Benralizumab
Antibody Quality and Performance Guarantee
A microtiter plate was coated overnight with various antigens at a concentration of 5 μg/mL. After washing and blocking with PBST + 5% BSA, detection was performed using Human Anti-Benralizumab Antibody, clone AbD60095ad (TZA068) at a concentration of 2 μg/mL followed by HRP conjugated Anti-DYKDDDDK Tag Antibody in HISPEC Assay Diluent (BUF049A) and QuantaBlu fluorogenic peroxidase substrate.
A microtiter plate was coated overnight with benralizumab, IL5Ra-His, human IgG1 kappa control and normal human serum (NHS) at a concentration of 5 µg/mL. After washing and blocking with PBST + 5% BSA, detection was performed using Human Anti-Benralizumab Antibody, clone AbD60095ad (TZA068) titrated to the given concentrations followed by an HRP conjugated Anti-DYKDDDDK Tag Antibody in HISPEC Assay Diluent (BUF049A) and QuantaBlu fluorogenic peroxidase substrate. Data for benralizumab are shown as the mean of three measurements.
A microtiter plate was coated overnight with Human Anti-Benralizumab Antibody, clone AbD60095ad (TZA068) at a concentration of 1 µg/mL. After washing and blocking with PBST + 5% BSA, 10% human serum was added spiked with increasing concentrations of benralizumab. Detection was performed using HRP-conjugated Human Anti-Benralizumab Antibody, clone AbD59973pap (TZA069P) at a concentration of 0.5 µg/mL in HISPEC Assay Diluent (BUF049A) and QuantaBlu fluorogenic peroxidase substrate. Data are shown as the mean of three measurements.
A microtiter plate was coated overnight with recombinant human IL5Ra-His at a concentration of 1 µg/mL. After washing and blocking with PBST + 5% BSA, a pre-incubated mixture of benralizumab (0.15 µg/mL) plus increasing concentrations of Human Anti-Benralizumab Antibody, clone AbD60095ad (TZA068). Free benralizumab, still capable of binding to the IL5Ra-His coated plate, was detected using HRP conjugated Mouse Anti-Human IgG (Fc) CH2 Domain Antibody (MCA647P) in HISPEC Assay Diluent (BUF049A) followed by QuantaBlu fluorogenic peroxidase substrate. Data are shown as the mean of three measurements.
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E Reset| Human Anti-Benralizumab Antibody, clone AbD60095ad is a paratope specific, anti-idiotypic antibody (Type 1) that specifically recognizes the free human monoclonal antibody drug benralizumab. It does not recognize the drug target, human interleukin 5 receptor α (IL5Rα), nor benralizumab in complex with IL5Rα. This antibody can be used to measure free benralizumab levels in serum from patients. Clone AbD60095ad is a monovalent Fab antibody incorporating a SpyTag at the C-terminus end of the antibody heavy chain for conversion by the end user into alternative formats using any of the SpyCatchers available in our catalog. A pair of anti-benralizumab antibodies can be used to develop a pharmacokinetic (PK) bridging assay to measure free drug; clone AbD60095ad is recommended as a capture antibody, paired with Human Anti-Benralizumab Antibody, clone AbD59973ad (TZA069) or clone AbD59973pap (TZA069P)as the detection reagent. Benralizumab (trade name Fasenra) is a humanized monoclonal antibody of the IgG1/kappa isotype that binds with high affinity to a conformationally distinct epitope within domain 1 of the α-chain of IL-5R, and thus inhibits proliferation of IL-5-dependent cell lines, and otherwise blocks signaling through the IL-5 receptor. Benralizumab is indicated as a maintenance treatment for severe asthma and an eosinophilic phenotype. View a summary of all anti-benralizumab antibodies |
- Product Form
- A monovalent human recombinant Fab (lambda light chain) selected from the HuCAL® phage display library, expressed in a proprietary E. coli. strain. The antibody is tagged with a DYKDDDDK tag, a SpyTag version 2 (VPTIVMVDAYKRYK) and a His6-tag (HHHHHH) at the C-terminus of the antibody heavy chain. This antibody is supplied as a liquid.
- Preparation
- Metal chelate affinity chromatography
- Source
- E.coli
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.0095% MIT
- Immunogen
- Benralizumab
- Affinity
- The monovalent intrinsic affinity of AbD60095ad was measured as KD = 0.2 nM by real time, label free molecular interaction analysis on immobilized benralizumab.
- Approx. Protein Concentrations
- Antibody concentration 0.5 mg/ml
- Regulatory
- For research purposes only
- Guarantee
- 12 months from date of despatch
- Acknowledgements
- This product and/or its use is covered by claims of U.S. patents, and/or pending U.S. and non-U.S. patent applications owned by or under license to Bio-Rad Laboratories, Inc. See bio-rad.com/en-us/trademarks for details.
His-tag is a registered trademark of EMD Biosciences.
Fasenra is a trademark of MedImmune, now owned by AstraZeneca. - Licensed Use
- For in vitro research purposes and for commercial applications for the provision of in vitro testing services to support preclinical and clinical drug development. Any re-sale in any form or any other commercial application needs a written agreement with Bio-Rad.
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| ELISA |  |
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
- Technical Advice
- Recommended protocols for coupling a Fab antibody with a SpyTag to a SpyCatcher can be found at SpyTag and SpyCatcher Products, and further information about HuCAL recombinant antibody technology can be found in the HuCAL Antibodies Technical Manual
- ELISA
- Clone AbD60095ad can be used in indirect ELISA to detect benralizumab. It can also be used as a capture antibody to develop a pharmacokinetic (PK) bridging ELISA for benralizumab together with TZA069 or TZA069P as the detection reagent.
Protocol: PK bridging ELISA
- Synonyms
- Fasenra
TZA068
171072
If you cannot find the batch/lot you are looking for please contact our technical support team for assistance.
View more products with Benralizumab specificity
Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"
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