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Atezolizumab antibody | AbD40640

Human anti Atezolizumab (Drug/Target Complex):HRP

Product Type
Monoclonal Antibody
Clone
AbD40640
Isotype
Fab antibody
Specificity
Atezolizumab
Region
DRUG/TARGET COMPLEX

Product Code Applications Pack Size List Price Your Price Qty
New TZA013P
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
E 0.1 mg loader
List Price Your Price
loader

Human Anti-Atezolizumab (Drug/Target Complex) Antibody, clone AbD40640 specifically recognizes the antibody drug atezolizumab when in complex with its target PD-L1 (programmed cell death ligand 1). It does not recognize free atezolizumab nor unbound PD-L1. It can be used to measure levels of atezolizumab in patient samples.

This TrailBlazer Antibody is available in a purified monovalent Fab format tagged with a SpyTag2 at the C terminus of the Fab antibody heavy chain (TZA013), enabling the user to couple it to a SpyCatcher reagent for conversion to alternative formats in less than one hour. It is also available as a bivalent Fab format pre-coupled to the an HRP-conjugated BiSpyCatcher2 for direct detection of atezolizumab under product code TZA013P.

View a summary of all SpyCatcher products

Either format can be used to develop a pharmacokinetic (PK) antigen capture assay to measure free atezolizumab captured on immobilized PD-L1.

Atezolizumab (brand name Tecentriq®) is a human monoclonal IgG1 antibody that binds directly to Programmed Death Ligand 1 (PD-L1, CD274) and provides a dual blockade of both the PD-1 and B7.1 receptors on T-cells and antigen presenting cells. It is one of the named “checkpoint inhibitor” drugs. PD-L1/PD-1 mediated inhibition of the immune response is thereby released, including reactivation of the anti-tumour immune response without induction of antibody-dependent cellular cytotoxicity. The drug is used to treat urothelial carcinoma, non-small cell lung cancer, triple-negative breast cancer, small cell lung cancer and hepatocellular carcinoma. It is used either on its own or in combination for cancers which are advanced or metastatic.

Product Form
A bivalent human recombinant Fab (lambda light chain) generated by coupling of the monovalent SpyTagged Fab TZA013 with a HRP conjugated BiSpyCatcher version 2 (TZC002P). The coupled antibody has two DYKDDDDK tags and three His6-tags. It contains SpyTag3 peptide (BLP086) in 5-fold molar excess to block any unreacted Catcher sites. This antibody is supplied as a liquid.
Preparation
Metal chelate affinity chromatography
Source
E.coli
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.0095% MIT
Immunogen
Atezolizumab/PD-L1 complex
Affinity
The monovalent intrinsic affinity of AbD40640pap was measured as KD = 91 nM by real time, label free molecular interaction analysis on immobilized PD-L1/atezolizumab complex.
Approx. Protein Concentrations
Antibody concentration 0.5 mg/ml
Regulatory
For research purposes only
Guarantee
12 months from date of despatch
Acknowledgements
This product and/or its use is covered by claims of U.S. patents, and/or pending U.S. and non-U.S. patent applications owned by or under license to Bio-Rad Laboratories, Inc. See bio-rad.com/en-us/trademarks for details.
His-tag is a registered trademark of EMD Biosciences.
Tecentriq is a trademark of Roche/ Genentech.

This product is shipped frozen.
When ready to use, thaw and aliquot the sample as needed. Store aliquots at -70°C, if available, otherwise store at -20°C. It is not recommended to keep aliquots at 4°C for more than one week.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Technical Advice
Recommended protocols for coupling a Fab antibody with a SpyTag to a SpyCatcher can be found at SpyTag and SpyCatcher Products, and further information about HuCAL recombinant antibody technology can be found in the HuCAL Antibodies Technical Manual
ELISA
Clone AbD40640pap may be used in a direct ELISA to detect the atezolizumab/PD-L1 complex or to detect atezolizumab bound to immobilized PD-L1.

Protocol: PK Antigen Capture ELISA

Description Product Code Applications Pack Size List Price Your Price Quantity
Hispec Assay Diluent BUF049A E IY 50 ml
List Price Your Price
Description Hispec Assay Diluent
Human anti Atezolizumab HCA380 E 0.1 mg loader
List Price Your Price
loader
Description Human anti Atezolizumab
Human anti Atezolizumab HCA381 E 0.1 mg loader
List Price Your Price
loader
Description Human anti Atezolizumab
Human anti Atezolizumab HCA382 E 0.1 mg loader
List Price Your Price
loader
Description Human anti Atezolizumab
Human anti Atezolizumab TZA011 E 0.1 mg
List Price Your Price
Description Human anti Atezolizumab
Human anti Atezolizumab (Drug/Target Complex) TZA013 E 0.1 mg
List Price Your Price
Description Human anti Atezolizumab (Drug/Target Complex)

Synonyms
Tecentriq
Licensed Use
For in vitro research purposes only, unless otherwise specified in writing by Bio-Rad.

TZA013P

166515

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Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"

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