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Atezolizumab antibody | AbD40638ad

Human anti Atezolizumab

Product Type
Monoclonal Antibody
Fab antibody

Product Code Applications Pack Size List Price Your Price Qty
New TZA011
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
E 0.1 mg loader
List Price Your Price

Human Anti-Atezolizumab Antibody, clone AbD40638ad is a paratope specific, inhibitory anti-idiotypic antibody (Type 1) that specifically recognizes the free monoclonal antibody drug atezolizumab. This is a humanized, Fc-engineered IgG1/kappa antibody with allotype nG1m1. The CH2 domain was engineered with one amino acid substitution (N297A) resulting in a non-glycosylated antibody, to impair Fcγ receptor binding and to prevent Fc-mediated depletion of cells expressing PD-L1.The antibody does not recognize free PD-L1 nor atezolizumab in complex with PD-L1.

Anti-Atezolizumab monovalent Fab antibody TZA011 has a SpyTag incorporated for conversion by the user into alternative antibody formats using any of the SpyCatchers available in our catalog.

View a summary of all SpyCatcher products

Clone AbD40638ad is also available in full length IgG1 format under product code HCA382.

Anti-Atezolizumab monovalent Fab antibody TZA011 may be used as the capture antibody for atezolizumab in a PK bridging ELISA together with HCA381, clone AbD40992ia as the detection antibody to measure free atezolizumab captured via immobilized PD-L1.

PK bridging ELISA

Atezolizumab (Tecentriq) is a human monoclonal IgG1 antibody that binds directly to Programmed Death Ligand 1 (PD-L1, CD274) and provides a dual blockade of both the PD-1 and B7.1 receptors on T-cells and antigen presenting cells. It is one of the named “checkpoint inhibitor” drugs. PD-L1/PD-1 mediated inhibition of the immune response is thereby released, including reactivation of the anti-tumour immune response without induction of antibody-dependent cellular cytotoxicity. The drug is used to treat urothelial carcinoma, non-small cell lung cancer, triple-negative breast cancer, small cell lung cancer and hepatocellular carcinoma. It is used either on its own or in combination for cancers which are advanced or metastatic.

View a summary of all anti-atezolizumab antibodies

Product Form
A monovalent human recombinant Fab (kappa light chain) selected from the HuCAL® phage display library, expressed in a proprietary E. coli strain. The antibody is tagged with a DYKDDDDK tag, a SpyTag version 2 (VPTIVMVDAYKRYK) and a His6-tag (HHHHHH) at the C-terminus of the antibody heavy chain. This antibody is supplied as a liquid.
Metal chelate affinity chromatography
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.0095% MIT
The monovalent intrinsic affinity of AbD40638ad was measured as KD = 0.4 nM by real time, label free molecular interaction analysis on immobilized atezolizumab.
Approx. Protein Concentrations
Antibody concentration 0.5 mg/ml
For research purposes only
12 months from date of despatch
This product and/or its use is covered by claims of U.S. patents, and/or pending U.S. and non-U.S. patent applications owned by or under license to Bio-Rad Laboratories, Inc. See for details.
His-tag is a registered trademark of EMD Biosciences.
Tecentriq is a trademerk of Roche/ Genentech.

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Technical Advice
Recommended protocols for coupling a Fab antibody with a SpyTag to a SpyCatcher can be found at SpyTag and SpyCatcher Products, and further information about HuCAL recombinant antibody technology can be found in the HuCAL Antibodies Technical Manual
Clone AbD40638ad can be used as the capture antibody for atezolizumab in a PK ELISA together with HCA381 as the detection antibody.

PK bridging ELISA

Description Product Code Applications Pack Size List Price Your Price Quantity
SpyTag3 Peptide BLP086 IY 1 mg loader
List Price Your Price
Description SpyTag3 Peptide
Hispec Assay Diluent BUF049A E IY 50 ml
List Price Your Price
Description Hispec Assay Diluent
Human anti Atezolizumab HCA380 E 0.1 mg
List Price Your Price
Description Human anti Atezolizumab
Human anti Atezolizumab HCA381 E 0.1 mg
List Price Your Price
Description Human anti Atezolizumab
Human anti Atezolizumab HCA382 E 0.1 mg
List Price Your Price
Description Human anti Atezolizumab
LYNX Rapid HRP Antibody Conjugation Kit LNK002P CJ 3 Conjugations For 400µg Antibody
List Price Your Price
Description LYNX Rapid HRP Antibody Conjugation Kit
BiSpyCatcher2:Biotin TZC002B IY 1 mg
List Price Your Price
Description BiSpyCatcher2:Biotin
BiSpyCatcher2:HRP TZC002P IY 1 mg
List Price Your Price
Description BiSpyCatcher2:HRP

Licensed Use
For in vitro research purposes and for commercial applications for the provision of in vitro testing services to support preclinical and clinical drug development. Any re-sale in any form or any other commercial application needs a written agreement with Bio-Rad.



If you cannot find the batch/lot you are looking for please contact our technical support team for assistance.

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Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"

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