Alemtuzumab antibody | AbD16942_hIgG1
A microtiter plate was coated overnight with various antigens at a concentration of 5 μg/ml. After washing and blocking with PBST + 5% milk powder, detection was performed using HRP conjugated Human Anti-Alemtuzumab Antibody, clone AbD16942 (HCA175P) at a concentration of 1 μg/ml in HISPEC Assay Diluent (BUF049A) and QuantaBlu Fluorogenic Peroxidase Substrate.
A microtiter plate was coated overnight with Human Anti-Alemtuzumab Antibody, clone AbD16728 (HCA174) at a concentration of 1 μg/ml. After washing and blocking with PBST + 5% BSA, 10% human serum was added spiked with increasing concentrations of alemtuzumab. Detection was performed using HRP conjugated Human Anti-Alemtuzumab Antibody, clone AbD16942_hIgG1 (HCA175P) at a concentration of 2 μg/ml in HISPEC Assay Diluent (BUF049A) followed by QuantaBlu Fluorogenic Peroxidase Substrate.
A microtiter plate was coated overnight with alemtuzumab at a concentration of 1.5 μg/ml. After washing and blocking with PBST + 5% BSA, PBST with 10% human serum was added spiked with increasing concentrations of Human Anti-Alemtuzumab Antibody, clone AbD16942_hIgG1 (HCA175). Detection was performed using Goat Anti-Human IgG F(ab’)2:HRP Antibody (STAR126P) followed by QuantaBlu Fluorogenic Peroxidase Substrate. Data are shown as the mean of three measurements.
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F E ResetHuman anti Alemtuzumab
- Product Type
- Monoclonal Antibody
- Clone
- AbD16942_hIgG1
- Isotype
- IgG1
- Specificity
- Alemtuzumab
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| Human anti Alemtuzumab, clone AbD16942_hIgG1, is an anti-idiotypic antibody that specifically recognizes the alemtuzumab humanized monoclonal antibody. Human anti Alemtuzumab, clone AbD16942_hIgG1 can be used to measure the levels of alemtuzumab and biosimilar products using bioanalytical assays. The HRP conjugated format of this antibody (HCA175P) may be used in a bridging ELISA with Human anti-alemtuzumab antibody, clone AbD16728. Alemtuzumab is a drug used in the treatment of chronic lymphocytic leukemia (CLL), cutaneous T-cell lymphoma (CTCL) and T-cell lymphoma. Alemtuzumab is also used in some conditioning regimes for bone marrow transplantation, kidney transplantation and Islet cell transplantation along with clinical trial protocols for treatment of certain autoimmune diseases including multiple sclerosis. Human anti Alemtuzumab, clone AbD16942_hIgG1 has been demonstrated to inhibit the binding of alemtuzumab to CD52-positive human cells in flow cytometry. Clone AbD16728 has been demonstrated to inhibit the binding of alemtuzumab to CD52-positive human cells in flow cytometry. It also blocks the binding of Rat anti Human CD52 antibody, clone YTH34.5 (MCA1642), the rat monoclonal prototype antibody of alemtuzumab, to CD52+ cells. View a summary of all anti-alemtuzumab antibodies. |
- Product Form
- Human IgG1 antibody (kappa light chain) selected from the HuCAL® phage display library and expressed in a human cell line.
- Preparation
- Purified IgG prepared by affinity chromatography on Protein A.
- Buffer Solution
- Phosphate buffered saline.
- Preservative Stabilisers
- 0.01% Thiomersal
- Immunogen
- Alemtuzumab.
- Affinity
- The monovalent intrinsic affinity of this antibody was measured as KD=2.4 nM by real time, label-free molecular interaction analysis on immobilized alemtuzumab.
- Approx. Protein Concentrations
- Total protein concentration 0.5 mg/ml.
- Regulatory
- For research purposes only
- Guarantee
- 12 months from date of despatch
- Acknowledgements
- This product and/or its use is covered by claims of U.S. patents, and/or pending U.S. and non-U.S. patent applications owned by or under license to Bio-Rad Laboratories, Inc. See bio-rad.com/en-us/trademarks for details.
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| ELISA |  |
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
- Technical Advice
- Recommended protocols and further information about HuCAL recombinant antibody technology can be found in the HuCAL Antibodies Technical Manual
- ELISA
- This product may be used in an indirect ELISA or as a detection antibody when conjugated to HRP in a bridging ELISA together with HCA174 as the capture reagent.
Protocol: PK bridging ELISA to measure free drug
This antibody is fully human and can be used as a reference standard in an ADA assay.
| Description | Product Code | Applications | Pack Size | List Price | Your Price | Quantity | |
|---|---|---|---|---|---|---|---|
| Hispec Assay Diluent | BUF049A | E IY | 50 ml | Log in | |||
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| Description | Hispec Assay Diluent | ||||||
| Human anti Alemtuzumab | HCA174 | E | 0.1 mg |
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| Description | Human anti Alemtuzumab | ||||||
| Rat anti Human CD52 | MCA1642 | C CT E F IP P RE WB | 0.2 mg |
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| Description | Rat anti Human CD52 | ||||||
References for Alemtuzumab antibody
-
Kashiwagi, N. et al. (2017) Method for measuring anti-drug antibody
US Patent Application US20170315118A1
- RRID
- AB_11152938
- Licensed Use
- For in vitro research purposes and for commercial applications for the provision of in vitro testing services to support preclinical and clinical drug development. Any re-sale in any form or any other commercial application needs a written agreement with Bio-Rad.
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