Alemtuzumab antibody | AbD16942_hIgG1
A microtiter plate was coated overnight with various antigens at a concentration of 5 μg/ml. After washing and blocking with PBST + 5% milk powder, detection was performed using HRP conjugated Human Anti-Alemtuzumab Antibody, clone AbD16942 (HCA175P) at a concentration of 1 μg/ml in HISPEC Assay Diluent (BUF049A) and QuantaBlu Fluorogenic Peroxidase Substrate.
A microtiter plate was coated overnight with Human Anti-Alemtuzumab Antibody, clone AbD16728 (HCA174) at a concentration of 1 μg/ml. After washing and blocking with PBST + 5% BSA, 10% human serum was added spiked with increasing concentrations of alemtuzumab. Detection was performed using HRP conjugated Human Anti-Alemtuzumab Antibody, clone AbD16942_hIgG1 (HCA175P) at a concentration of 2 μg/ml in HISPEC Assay Diluent (BUF049A) followed by QuantaBlu Fluorogenic Peroxidase Substrate.
A microtiter plate was coated overnight with alemtuzumab at a concentration of 1.5 μg/ml. After washing and blocking with PBST + 5% BSA, PBST with 10% human serum was added spiked with increasing concentrations of Human Anti-Alemtuzumab Antibody, clone AbD16942_hIgG1 (HCA175). Detection was performed using Goat Anti-Human IgG F(ab’)2:HRP Antibody (STAR126P) followed by QuantaBlu Fluorogenic Peroxidase Substrate. Data are shown as the mean of three measurements.
Human anti Alemtuzumab:HRP
Anti-alemtuzumab antibody is a recombinant, neutralizing anti-idiotypic antibody in fully human IgG1 format; it is suitable for bioanalytical method development for alemtuzumab or biosimilars. It is recommended as a the detection antibody in a PK bridging ELISA.
Human anti Alemtuzumab
Anti-alemtuzumab antibody is a recombinant, neutralizing anti-idiotypic antibody in fully human IgG1 format; it is suitable for bioanalytical method development for alemtuzumab or biosimilars. It is recommended as a the detection antibody in a PK bridging ELISA.
- Product Type
- Monoclonal Antibody
- Clone
- AbD16942_hIgG1
- Isotype
- IgG1
| Product Code | Applications | Pack Size | List Price | Quantity |
|---|---|---|---|---|
| HCA175P | E | 0.1 mg |
|
|
| HCA175 | E | 0.1 mg |
|
Human anti Alemtuzumab, clone AbD16942_hIgG1, is an anti-idiotypic antibody that specifically recognizes the alemtuzumab humanized monoclonal antibody.
Human anti Alemtuzumab, clone AbD16942_hIgG1 can be used to measure the levels of alemtuzumab and biosimilar products using bioanalytical assays. The HRP conjugated format of this antibody (HCA175P) may be used in a bridging ELISA with Human anti-alemtuzumab antibody, clone AbD16728.
Alemtuzumab is a drug used in the treatment of chronic lymphocytic leukemia (CLL), cutaneous T-cell lymphoma (CTCL) and T-cell lymphoma. Alemtuzumab is also used in some conditioning regimes for bone marrow transplantation, kidney transplantation and Islet cell transplantation along with clinical trial protocols for treatment of certain autoimmune diseases including multiple sclerosis.
Human anti Alemtuzumab, clone AbD16942_hIgG1 has been demonstrated to inhibit the binding of alemtuzumab to CD52-positive human cells in flow cytometry. Clone AbD16728 has been demonstrated to inhibit the binding of alemtuzumab to CD52-positive human cells in flow cytometry. It also blocks the binding of Rat anti Human CD52 antibody, clone YTH34.5 (MCA1642), the rat monoclonal prototype antibody of alemtuzumab, to CD52+ cells.
View a summary of all anti-alemtuzumab antibodies.
Human anti Alemtuzumab, clone AbD16942_hIgG1 can be used to measure the levels of alemtuzumab and biosimilar products using bioanalytical assays. The HRP conjugated format of this antibody (HCA175P) may be used in a bridging ELISA with Human anti-alemtuzumab antibody, clone AbD16728.
Alemtuzumab is a drug used in the treatment of chronic lymphocytic leukemia (CLL), cutaneous T-cell lymphoma (CTCL) and T-cell lymphoma. Alemtuzumab is also used in some conditioning regimes for bone marrow transplantation, kidney transplantation and Islet cell transplantation along with clinical trial protocols for treatment of certain autoimmune diseases including multiple sclerosis.
Human anti Alemtuzumab, clone AbD16942_hIgG1 has been demonstrated to inhibit the binding of alemtuzumab to CD52-positive human cells in flow cytometry. Clone AbD16728 has been demonstrated to inhibit the binding of alemtuzumab to CD52-positive human cells in flow cytometry. It also blocks the binding of Rat anti Human CD52 antibody, clone YTH34.5 (MCA1642), the rat monoclonal prototype antibody of alemtuzumab, to CD52+ cells.
View a summary of all anti-alemtuzumab antibodies.
Product Details
- Product Form
- Human IgG1 antibody (kappa light chain) selected from the HuCAL® phage display library and expressed in a human cell line. Conjugated to horseradish peroxidase (HRP) - liquid.
- Product Form
- Human IgG1 antibody (kappa light chain) selected from the HuCAL® phage display library and expressed in a human cell line.
- Preparation
- Purified IgG prepared by affinity chromatography on Protein A.
- Preparation
- Purified IgG prepared by affinity chromatography on Protein A.
- Buffer Solution
- Phosphate buffered saline.
- Buffer Solution
- Phosphate buffered saline.
- Preservative Stabilisers
- 0.01% Thiomersal
- Preservative Stabilisers
- 0.01% Thiomersal
- Immunogen
- Alemtuzumab.
- Affinity
- The monovalent intrinsic affinity of this antibody was measured as KD=2.4 nM by real time, label-free molecular interaction analysis on immobilized alemtuzumab.
- Approx. Protein Concentrations
- Total protein concentration 0.1 mg/ml.
- Approx. Protein Concentrations
- Total protein concentration 0.5 mg/ml.
Storage Information
- Storage
- Store at -70oC.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. - Storage
- This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up tp 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. - Guarantee
- 12 months from date of despatch
- Guarantee
- 12 months from date of despatch
More Information
- Acknowledgements
- Sold under license of U.S. Patents 6753136, 7785859 and 8273688 and corresponding patents. This antibody was developed by Bio-Rad, Zeppelinstr. 4, 82178 Puchheim, Germany.
- Acknowledgements
- Sold under license of U.S. Patents 6753136, 7785859 and 8273688 and corresponding patents. This antibody was developed by Bio-Rad, Zeppelinstr. 4, 82178 Puchheim, Germany.
- Licensed Use
- For in vitro research purposes and for commercial applications for the provision of in vitro testing services to support preclinical and clinical drug development. Any re-sale in any form or any other commercial application needs a written agreement with Bio-Rad.
- Regulatory
- For research purposes only
Applications of Alemtuzumab antibody
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| ELISA | |||
| ELISA |
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
- Technical Advice
- Recommended protocols and further information about HuCAL recombinant antibody technology can be found in the HuCAL Antibodies Technical Manual
- Technical Advice
- Recommended protocols and further information about HuCAL recombinant antibody technology can be found in the HuCAL Antibodies Technical Manual
- ELISA
- This product may be used in a direct ELISA or as a detection reagent in a sandwich ELISA together with HCA174 as the capture reagent.
Protocol: PK bridging ELISA to measure free drug - ELISA
- This product may be used in an indirect ELISA or as a detection antibody when conjugated to HRP in a bridging ELISA together with HCA174 as the capture reagent.
Protocol: PK bridging ELISA to measure free drug
This antibody is fully human and can be used as a reference standard in an ADA assay.
Copyright © 2021 Bio-Rad Antibodies (formerly AbD Serotec)
Useful Reagents Available
| Description | Product Code | Applications | Pack Size | List Price | Quantity |
|---|---|---|---|---|---|
| Hispec Assay Diluent | BUF049A | E IY | 50 ml | ||
| Human anti Alemtuzumab | HCA174 | E | 0.1 mg |
|
|
| Rat anti Human CD52 | MCA1642 | C CT E F IP P RE WB | 0.2 mg |
|
|
| Hispec Assay Diluent | BUF049A | E IY | 50 ml | ||
| Human anti Alemtuzumab | HCA174 | E | 0.1 mg |
|
|
| Rat anti Human CD52 | MCA1642 | C CT E F IP P RE WB | 0.2 mg |
|
Product Specific References
References for Alemtuzumab antibody
-
Kashiwagi, N. et al. (2017) Method for measuring anti-drug antibody
US Patent Application US20170315118A1
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