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Alemtuzumab Antibody | AbD16728

Alemtuzumab Antibody | AbD16728 gallery image 1

HCA174 specificity ELISA using various antigens (A: BSA, B: CD33, C: GST, D: Human serum, E: Trastuzumab, F: Bevacizumab, G: Alemtuzumab) as coating components; Human anti Alemtuzumab (HCA174) as the primary antibody and Mouse anti Strep-tag:HRP (MCA2489P) for detection

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Alemtuzumab bridging ELISA using Human anti Alemtuzumab (HCA174) as the coating antibody; Alemtuzumab as the antigen and Human anti Alemtuzumab:HRP (HCA175P) as the detection antibody

Alemtuzumab Antibody | AbD16728 gallery image 3

Binding of FITC conjugated Alemtuzumab to human lymphocytes (blue unshaded histogram) and subsequent inhibition of binding (red shaded histogram) in the presence of a 5 fold molar excess of HCA174 (A) or the corresponding negative control (B)

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  • Human anti Alemtuzumab
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    Anti-alemtuzumab antibody is a recombinant, neutralizing anti-idiotypic antibody in monovalent Fab format; it is ideal for assay development to measure levels of alemtuzumab or biosimilars. It is recommended as a capture antibody in a PK bridging ELISA.
    • Product Type
      Monoclonal Antibody
    • Clone
    • Isotype
      HuCAL Fab monovalent
    1 Formats Available
      Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
      HCA174Edatasheet pdfdatasheet pdf0.1 mg
      Secondary Antibodies
      Negative Isotype Controls
      Useful Reagents
      Positive Controls
      Histology Controls
      More Images
      • Human anti Alemtuzumab antibody, clone AbD16728 recognizes alemtuzumab, a monoclonal antibody drug directed to human CD52 and used in the treatment of chronic lymphocytic leukemia (CLL), cutaneous T-cell lymphoma (CTCL) and T-cell lymphoma. Alemtuzumab is also used in some conditioning regimes for bone marrow transplantation, kidney transplantation and Islet cell transplantation along with clinical trial protocols for treatment of certain autoimmune diseases including multiple sclerosis.

        Clone AbD16728 is an anti-idiotypic antibody that specifically recognizes the alemtuzumab humanized monoclonal antibody and and can be used to measure the levels of alemtuzumab and biosimilar products using bioanalytical assays.

        This antibody may be used in a bridging ELISA in conjunction with horseradish peroxidase conjugated clone AbD16942 (HCA175P).

        Clone AbD16728 has been demonstrated to inhibit the binding of alemtuzumab to CD52-positive human cells in flow cytometry. It also blocks the binding of Rat anti Human CD52 antibody, clone YTH34.5, the Rat monoclonal prototype antibody of alemtuzumab, to CD52+ cells.

        View a summary of all anti-alemtuzumab antibodies.
      • Intended Use
      • Product Form
        A monovalent human recombinant Fab selected from the HuCAL® phage display library, expressed in E. coli. The antibody is tagged with a V5 tag and a double extended Strep-tag. This antibody is supplied as a liquid.
      • Reconstitution
      • Preparation
        StrepTactin affinity chromatography.
      • Preservative Stabilisers
        0.09% Sodium Azide (NaN3).
      • Immunogen
      • Purity
      • Affinity
        The monovalent intrinsic affinity of this antibody was measured as KD=1.6 nM by real time, label-free molecular interaction analysis on immobilized alemtuzumab.
      • Approx. Protein Concentrations
        Total protein concentration 0.5 mg/ml.
      • Reagents In The Kit
      • Preparing The Antibody
      • Test Principle
      • Buffer Solution
        Phosphate buffered saline.
      • Storage
        Store at +4oC or at -20oC if preferred.
        Storage in frost-free freezers is not recommended.
        This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
      • Shelf Life
        12 months from date of despatch.
      • Acknowledgements
        Sold under license of U.S. Patents 6,300,064, 6,696,248, 6,708,484, 6,753,136, European Patent 0,859,841 and corresponding patents.This antibody was developed by Bio-Rad, a wholly owned subsidiary of Bio-Rad Laboratories Inc., Zeppelinstr. 4, 82178 Puchheim, Germany.
      • Licensed Use
        For in vitro research purposes and for commercial applications for the provision of in vitro testing services to support preclinical and clinical drug development. Any re-sale in any form or any other commercial application needs a written agreement with Bio-Rad.
      • Regulatory
        For research purposes only
      • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

      • Application NameYesNoMin DilutionMax Dilution

      • Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
      • Technical Advice
        Recommended protocols and further information about HuCAL recombinant antibody technology can be found in the HuCAL Antibodies Technical Manual
      • Recommended Protocol
      • ELISA
        This product may be used in an indirect ELISA or as a capture antibody in a sandwich ELISA together with HCA175P as the detection reagent. Protocol: PK bridging ELISA to measure free drug
      • Immunohistology
      • Histology Positive Control Tissue
      • Immunofluorescence
      • Western Blotting
      • Instructions For Use

      Additional Alemtuzumab Antibody Formats

      Formats Clone Applications Sizes available
      Alemtuzumab Antibody : Purified AbD16728 E 0.1 mg
      • Copyright © 2016 Bio-Rad

      Recommended Secondary Antibody

        DescriptionProduct CodePack SizeApplicationsList PriceQuantity
        Mouse anti V5-Tag:BiotinMCA1360B0.1 mgC, E, WB
        Mouse anti V5-Tag:HRPMCA1360P0.1 mgE, WB
        Mouse anti Strep-Tag Classic:HRPMCA2489P75 µlE, WB
        Mouse anti Strep-Tag ImmoMCA24880.1 mgE
        Mouse anti Strep-Tag ClassicMCA24890.1 mgE, WB
        Mouse anti V5-TagMCA13601 mgC, E, F, IF, IP, R, WB

        Recommended Negative Isotype Control

          Useful Reagents

            DescriptionProduct CodePack SizeApplicationsList PriceQuantity
            Human anti Alemtuzumab:HRPHCA175P0.1 mgE
            Rat anti Human CD52MCA16420.2 mgC, CT, E, F, IP, P, RE, WB
            Hispec Assay DiluentBUF049A50 mlIY

            Recommended Positive Controls

              Histology Controls

                  • Product Images

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