Abatacept antibody | AbD37065ia
A microtiter plate was coated overnight with recombinant human CD80-Fc at a concentration of 5 µg/ml. After washing and blocking with PBST + 5% BSA, a pre-incubated mixture of HRP conjugated abatacept (1 µg/ml) plus increasing concentrations of Human Anti-Abatacept Antibody, clone AbD37065ia (HCA336) in the monovalent Fab format was added. Free HRP conjugated abatacept, still capable of binding to the human CD80-Fc coated plate, was detected using QuantaBlu Fluorogenic Peroxidase Substrate. Data are shown as the mean of three measurements. HRP conjugation of the antibody used in this assay was performed using a LYNX Rapid HRP Antibody Conjugation Kit (LNK002P).
A microtiter plate was coated overnight with various antigens at a concentration of 5 μg/ml. After washing and blocking with PBST + 5% non-fat dry milk, detection was performed using HRP conjugated Human Anti-Abatacept antibody, clone AbD37065ia (HCA336) at a concentration of 2 μg/mL. Detection was performed in HISPEC Assay Diluent (BUF049A) followed by QuantaBlu Fluorogenic Peroxidase Substrate. HRP-conjugation of the antibody used in this assay was performed using a LYNX Rapid HRP Antibody Conjugation Kit (LNK002P).
A microtiter plate was coated overnight with belatacept, abatacept, human IgG1 kappa control or human Fc-tag (same allotype as Belatacept and Abatacept) at a concentration of 5 µg/ml. After washing and blocking with PBST + 5% BSA, detection was performed using HRP conjugated Human Anti-Abatacept, clone AbD37065ia (HCA336) titrated to the given concentrations in HISPEC diluent (BUF049A) followed by QuantaBlu Fluorogenic Peroxidase Substrate. Data are shown as the mean of three measurements. HRP-conjugation of the antibody used in this assay was performed using a LYNX Rapid HRP Antibody Conjugation Kit (LNK002P).
A microtiter plate was coated overnight with belatacept at a concentration of 1 µg/ml. After washing and blocking with PBST + 5% BSA, PBST with 10% human serum was added spiked with increasing concentrations of Human Anti-Belatacept Antibody, clone AbD37060ia (HCA392, black) and clone AbD36935ia (HCA393, blue) or Human Anti-Abatacept Antibody, clone AbD37065ia (HCA336, red). Detection was performed using HRP conjugated belatacept at a concentration of 2 µg/ml in HISPEC Assay Diluent (BUF049A) followed by QuantaBlu Fluorogenic Peroxidase Substrate. HRP conjugation of the antibody used in this assay was performed using a LYNX Rapid HRP Antibody Conjugation Kit (LNK002P). Data are shown as the mean of three measurements.
Human anti Abatacept
- Product Type
- Monoclonal Antibody
- Clone
- AbD37065ia
- Isotype
- IgG1
- Specificity
- Abatacept
Antibody Quality and Performance Guarantee
| Human Anti-Abatacept, clone AbD37065ia is a recombinant antibody that specifically recognizes the fusion protein drugs abatacept, belatacept as well as cytotoxic T-lymphocyte antigen-4 (CTLA-4), also known as CD152. The antibody binds to free abatacept and belatacept, and can be used to measure free abatacept, abatacept biosimilars, belatacept and belatacept biosimilars in patient samples. Clone AbD37065ia is a fully human recombinant monoclonal antibody with an IgG1 isotype and is suitable as a surrogate positive control or reference standard in an anti-drug antibody (ADA) assay. Abatacept (Orencia) is a recombinant fusion protein consisting of the extra-cellular domain of human CTLA-4, fused to the modified Fc (hinge, CH2, and CH3 domains) portion of human IgG1. It is used to treat several autoimmune diseases, including rheumatoid arthritis, psoriatic arthritis and juvenile idiopathic arthritis, by inhibiting optimal T-cell activation. It binds to CD80 and CD86 on antigen-presenting cells and blocks the costimulatory interaction with CD28 on T cells. View a summary of all anti-abatacept antibodies |
- Product Form
- Human IgG1 antibody (kappa light chain) selected from the HuCAL phage display library and expressed in a human cell line. This antibody is supplied as a liquid.
- Preparation
- Purified IgG prepared by affinity chromatography on Protein A
- Source
- HKB-11
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.09% Sodium Azide (NaN3)
- Immunogen
- Abatacept
- Affinity
- The intrinsic affinity of the monovalent form of Human anti Abatacept antibody, clone AbD37065ia is KD = 6 nM as measured by real time, label free molecular interaction analysis on immobilized Abatacept.
- Approx. Protein Concentrations
- Antibody concentration 0.5 mg/ml
- Regulatory
- For research purposes only
- Guarantee
- 12 months from date of despatch
- Acknowledgements
- This product and/or its use is covered by claims of U.S. patents, and/or pending U.S. and non-U.S. patent applications owned by or under license to Bio-Rad Laboratories, Inc. See bio-rad.com/en-us/trademarks for details.
Orencia is a trademark of Bristol-Myers Squibb Company.
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| ELISA |  |
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
- Technical Advice
- Recommended protocols and further information about HuCAL recombinant antibody technology can be found in the HuCAL Antibodies Technical Manual.
- ELISA
- Human anti Abatacept antibody, clone AbD37065ia can be used in an indirect ELISA. It can also be used as a calibrator in an anti-drug antibody assay.
Protocol: ADA bridging ELISA.
| Description | Product Code | Applications | Pack Size | List Price | Your Price | Quantity | |
|---|---|---|---|---|---|---|---|
| Hispec Assay Diluent | BUF049A | E IY | 50 ml | Log in | |||
| List Price | Your Price | ||||||
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| Description | Hispec Assay Diluent | ||||||
| Human anti Abatacept | HCA335 | E | 0.1 mg |
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| List Price | Your Price | ||||||
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| Description | Human anti Abatacept | ||||||
| LYNX Rapid HRP Antibody Conjugation Kit | LNK002P | CJ | 3 Conjugations For 400µg Antibody | Log in | |||
| List Price | Your Price | ||||||
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| Description | LYNX Rapid HRP Antibody Conjugation Kit | ||||||
- Synonyms
- Orencia
- Licensed Use
- For in vitro research purposes and for commercial applications for the provision of in vitro testing services to support preclinical and clinical drug development. Any re-sale in any form or any other commercial application needs a written agreement with Bio-Rad.
HCA336
157177
164792
164969
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