Laminin antibody
Rabbit anti Laminin antibody (AHP420) used to evaluate laminin expression on CA1a breast cancer cells using immunofluorescence.
Image caption:
Matrix stiffening induces mechanosensitive signalling promoting a malignant phenotype in breast cancer cells.
Spinning disc confocal immunofluorescent images of CA1a cells on 0.4 kPa and 5 kPa. Scale bar = 20 μm.
From: Göransson, S et al.
Extracellular matrix stiffness-induced breast cancer cell transcriptome resembles the transition from ductal carcinoma in situ (DCIS) to invasive ductal carcinoma (IDC).
Biochem Biophys Ras Comms. 2 Mar [Epub ahead of print].
This image is from an open access article distributed under terms of a Creative Commons Attribution License.
Rabbit anti Laminin
- Product Type
- Polyclonal Antibody
- Isotype
- Polyclonal IgG
- Specificity
- Laminin
Antibody Quality and Performance Guarantee
| Rabbit anti Laminin antibody recognizes both A (~400 kDa) and B (~220 kDa) chains of laminin. |
- Species Cross-Reactivity
-
Target Species Cross Reactivity Rabbit Chicken Duck Birds Expected from Sequence Human Dog Pigeon - N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Whole serum - liquid
- Antiserum Preparation
- Antisera to laminin were raised by repeated immunisation of rabbits with highly purified antigen.
- Preservative Stabilisers
0.09% Sodium Azide - Immunogen
- Purified laminin from Engelbreth-Holm-Swarn (EHS) murine sarcoma.
- Regulatory
- For research purposes only
- Guarantee
- 12 months from date of despatch
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| ELISA |  |
>1/1000 | |
| Immunofluorescence | |
||
| Immunohistology - Frozen | |
1/200 | 1/1000 |
| Immunohistology - Paraffin | |
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
- Histology Positive Control Tissue
- Human kidney
| Description | Product Code | Applications | Pack Size | List Price | Your Price | Quantity | |
|---|---|---|---|---|---|---|---|
| Goat anti Rabbit IgG (Fc):Biotin | STAR121B | E WB | 1 mg |
|
Log in | ||
| List Price | Your Price | ||||||
|
|
Log in | ||||||
| Description | Goat anti Rabbit IgG (Fc):Biotin | ||||||
| Goat anti Rabbit IgG (Fc):FITC | STAR121F | F | 1 mg |
|
Log in | ||
| List Price | Your Price | ||||||
|
|
Log in | ||||||
| Description | Goat anti Rabbit IgG (Fc):FITC | ||||||
| Goat anti Rabbit IgG (Fc):HRP | STAR121P | E WB | 1 mg |
|
Log in | ||
| List Price | Your Price | ||||||
|
|
Log in | ||||||
| Description | Goat anti Rabbit IgG (Fc):HRP | ||||||
| Goat anti Rabbit IgG (H/L):HRP | STAR124P | C E WB | 1 mg |
|
Log in | ||
| List Price | Your Price | ||||||
|
|
Log in | ||||||
| Description | Goat anti Rabbit IgG (H/L):HRP | ||||||
| Sheep anti Rabbit IgG:RPE | STAR35A | F | 1 ml |
|
Log in | ||
| List Price | Your Price | ||||||
|
|
Log in | ||||||
| Description | Sheep anti Rabbit IgG:RPE | ||||||
References for Laminin antibody
-
Gordon-Weeks, P.R. et al. (1989) Transient expression of laminin immunoreactivity in the developing rat hippocampus.
J Neurocytol. 18 (4): 451-63. -
Gordon-Weeks, P.R. et al. (1992) A study of the expression of laminin in the spinal cord of the frog during development and regeneration.
Exp Physiol. 77 (5): 681-92. -
Falk, V. et al. (2002) Regulation of matrix metalloproteinases and effect of MMP-inhibition in heart transplant related reperfusion injury.
Eur J Cardiothorac Surg. 22: 53-8. -
Mueller, S.N. et al. (2007) Viral targeting of fibroblastic reticular cells contributes to immunosuppression and persistence during chronic infection.
Proc Natl Acad Sci U S A. 104: 15430-5. -
Au, E. et al. (2007) SPARC from olfactory ensheathing cells stimulates Schwann cells to promote neurite outgrowth and enhances spinal cord repair.
J Neurosci. 27: 7208-21. -
Miners, J.S. et al. (2008) Angiotensin-converting enzyme (ACE) levels and activity in Alzheimer's disease, and relationship of perivascular ACE-1 to cerebral amyloid angiopathy.
Neuropathol Appl Neurobiol. 34: 181-93. -
Melo, F.A. et al. (2009) Hepatic extracellular matrix alterations in dogs naturally infected with Leishmania (Leishmania) chagasi.
Int J Exp Pathol. 90: 538-48. -
Richardson, G.D. et al. (2009) Dynamic expression of Syndecan-1 during hair follicle morphogenesis.
Gene Expr Patterns. 9: 454-60.
View The Latest Product References
-
Abd-Elmaksoud, A. et al. (2009) Comparative expression of laminin and smooth muscle actin in the testis and epididymis of poultry and rabbit.
J Mol Histol. 40: 407-16. -
Arnold, S.A. et al. (2012) Losartan Slows Pancreatic Tumor Progression and Extends Survival of SPARC-Null Mice by Abrogating Aberrant TGFβ Activation.
PLoS One. 7(2):e31384. -
Yeh, T.S. et al. (2014) Baculovirus-transduced, VEGF-expressing adipose-derived stem cell sheet for the treatment of myocardium infarction.
Biomaterials. 35 (1): 174-84. -
Kato, Y. et al. (2015) Targeting Antigen to Clec9A Primes Follicular Th Cell Memory Responses Capable of Robust Recall.
J Immunol. 195 (3): 1006-14. -
Foditsch, E.E. et al. (2016) Skeletal muscle proteins: a new approach to delimitate the time since death.
Int J Legal Med. 130 (2): 433-40. -
Göransson, S. et al. (2023) An extracellular matrix stiffness-induced breast cancer cell transcriptome resembles the transition from ductal carcinoma in situ (DCIS) to invasive ductal carcinoma (IDC).
Biochem Biophys Res Commun. 654: 73-9. -
Kato, Y. et al. (2020) Display of Native Antigen on cDC1 That Have Spatial Access to Both T and B Cells Underlies Efficient Humoral Vaccination.
J Immunol. 205 (7): 1842-56.
- RRID
- AB_2133777
AHP420T
1608
161570
AHP420
151842
If you cannot find the batch/lot you are looking for please contact our technical support team for assistance.
View more products with LAMININ specificity
Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"
Always be the first to know.
When we launch new products and resources to help you achieve more in the lab.
Yes, sign me up