WNT-3a Antibody

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WNT-3a Antibody gallery image 1

Formlin fixed, paraffin embedded human breast invasive ductal carcinoma stained with Rabbit anti Human WNT-3a (AHP2188) following heat mediated antigen retrieval using sodium citrate buffer (pH6.0)

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WNT-3a Antibody gallery image 2

Formlin fixed, paraffin embedded human breast invasive ductal carcinoma stained with Rabbit anti Human WNT-3a (AHP2188) following heat mediated antigen retrieval using sodium citrate buffer (pH6.0)

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  • Rabbit anti Human WNT-3a
  • Rabbit anti Human WNT-3a:Biotin
(Rated 0.0 out of 5 based on 0 customer reviews)
  • Product Type
    Polyclonal Antibody
  • Isotype
    Polyclonal IgG
2 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    AHP2188E, P *, WBdatasheet pdfdatasheet pdf0.1 mg
    AHP2188
    AHP2188BE, WBdatasheet pdfdatasheet pdf50 µg
    AHP2188B
    Summary
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    More Images
    References
    Reviews
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    • Rabbit anti Human WNT-3a antibody specifically recognizes human WNT-3a, a secreted protein and member of the Wnt family of signalling proteins, which acts as a ligand for members of the frizzled family of seven transmembrane receptors, and plays key roles in many developmental and cell fate decisions, including embryonic patterning, CNS development, and cytoskeletal formation.

      Wnt-3a acts as a promoter of the proliferation of ventral midbrain dopaminergic neuron precursor cells, and has been implicated in the progressive differentiation of anterior neural cells, as well as regulating the survival and expansion of neural progenitors derived from human embryonic stem cells. Wnt3a has been shown to act upstream of the Notch signalling pathway, in the regulation of somitogenesis, which is believed to be controlled by a segmentation clock, consisting of molecular oscillators in the Notch, Wnt3a and Fgf8 pathways.
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution
      ELISA0.52.0ug/ml
      Western Blotting0.10.2ug/ml
      Immunohistology - Paraffin(1)
      (1)
      This product requires antigen retrieval using heat treatment prior to staining of paraffin sections. Sodium citrate buffer pH 6.0 is recommended for this purpose.
    • Application NameYesNoMin DilutionMax Dilution
      ELISA0.251.0ug/ml
      Western Blotting0.10.2ug/ml

    • Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
    • Technical Advice
    • Technical Advice
    • Recommended Protocol
    • Recommended Protocol
    • ELISA
      AHP2188 may be used in an indirect ELISA or as a capture reagent in a sandwich ELISA with (AHP2188B) as the detection antibody.
    • ELISA
      AHP2188B may be used in a direct ELISA or as the detection antibody in a sandwich ELISA with (AHP2188) as the capture reagent.
    • Immunohistology
    • Immunohistology
    • Histology Positive Control Tissue
    • Histology Positive Control Tissue
    • Immunofluorescence
    • Immunofluorescence
    • Western Blotting
    • Western Blotting
    • Instructions For Use
    • Instructions For Use

    Additional WNT-3a Antibody Formats

    Formats Applications Sizes available
    WNT-3a Antibody : Purified E, P *, WB 0.1 mg
    WNT-3a Antibody : Biotin E, WB 50 µg
    • Copyright © 2016 Bio-Rad

    Recommended Secondary Antibody

      DescriptionProduct CodePack SizeApplicationsList PriceQuantity
      Goat anti Rabbit IgG (Fc):BiotinSTAR121B1 mgE, WB
      STAR121B
      Sheep anti Rabbit IgG:Biotin2AB02B1 mlC, E, P, WB
      2AB02B
      Sheep anti Rabbit IgG:DyLight®488STAR36D488GA0.1 mgF, IF
      STAR36D488GA
      Sheep anti Rabbit IgG:DyLight®549STAR36D549GA0.1 mgF, IF
      STAR36D549GA
      Sheep anti Rabbit IgG:DyLight®649STAR36D649GA0.1 mgF, IF
      STAR36D649GA
      Sheep anti Rabbit IgG:Dylight®800STAR36D800GA0.1 mgF, IF, WB
      STAR36D800GA
      Goat anti Rabbit IgG (Fc):FITCSTAR121F1 mgF
      STAR121F
      Sheep anti Rabbit IgG:FITCSTAR34B1 mgC, F
      STAR34B
      Goat anti Rabbit IgG (Fc):HRPSTAR121P1 mgE, WB
      STAR121P
      Goat anti Rabbit IgG (H/L):HRPSTAR124P1 mgC, E, WB
      STAR124P
      Sheep anti Rabbit IgG:HRP (Multi Adsorbed)STAR541 mgC, E, WB
      STAR54
      Sheep anti Rabbit IgG:RPESTAR35A1 mlF
      STAR35A
      DescriptionProduct CodePack SizeApplicationsList PriceQuantity
      Streptavidin:APCSTAR119100 TestsF
      STAR119

      Recommended Negative Isotype Control

        Useful Reagents

          DescriptionProduct CodePack SizeApplicationsList PriceQuantity
          Antigen Retrieval Buffer, pH8.0BUF025C50 mlP
          BUF025C
          TidyBlot Western Blot Detection Reagent:HRPSTAR209P0.5 mlWB*
          STAR209P
          Antigen Retrieval Buffer, pH8.0BUF025A500 mlP
          BUF025A

          Recommended Positive Controls

            Histology Controls

              Further Reading

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