TNF Beta antibody
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Rabbit anti Human TNF Beta
- Product Type
- Polyclonal Antibody
- Isotype
- Polyclonal IgG
- Specificity
- TNF Beta
Antibody Quality and Performance Guarantee
| Rabbit anti Human TNF beta antibody recognizes human tumour necrosis factor beta (TNF-beta), also known as lymphotoxin alpha (LT-alpha), an 18.6 kDa homotrimeric polypeptide secreted by activated T and B lymphocytes, which binds to the TNF receptors TNFR1, TNFR2 and HVEM (herpes virus entry mediator receptor). TNF-beta was originally isolated due to its cytotoxic effects on tumour cells, and has since been identified as a regulator of many cellular processes and a potent mediator of inflammation and immune responses against invading pathogens. |
- Target Species
- Human
- Product Form
- Purified IgG - lyophilized
- Reconstitution
- Reconstitute with 0.1ml distilled water . For long term storage the addition of 0.09% sodium azide is recommended.
- Antiserum Preparation
- Antisera to human TNF-beta were raised by repeated immunisation of rabbits with highly purified antigen. Purified IgG prepared by antigen affinity chromatography.
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- None present
- Carrier Free
- Yes
- Immunogen
- Recombinant human TNF-beta.
- Approx. Protein Concentrations
- IgG concentration 1.0 mg/ml
- Regulatory
- For research purposes only
- Guarantee
- 12 months from date of despatch
Prior to reconstitution store at -20°C.
After reconstitution store at -20°C.
This product should be stored undiluted. Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
After reconstitution store at -20°C.
This product should be stored undiluted. Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| ELISA |  |
0.5ug/ml | |
| Western Blotting | |
0.1 | 0.2ug/ml |
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
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References for TNF Beta antibody
-
Pfeffer, K. (2003) Biological functions of tumor necrosis factor cytokines and their receptors.
Cytokine Growth Factor Rev. 14 (3-4): 185-91.
- Synonyms
- LT-ALPHA
- RRID
- AB_2138634
- UniProt
- P01374
- Entrez Gene
- LTA
- GO Terms
- GO:0016020 membrane
- GO:0007165 signal transduction
- GO:0005125 cytokine activity
- GO:0005164 tumor necrosis factor receptor binding
- GO:0005615 extracellular space
- GO:0006917 induction of apoptosis
- GO:0007267 cell-cell signaling
AHP355G
159069
164849
If you cannot find the batch/lot you are looking for please contact our technical support team for assistance.
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Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"
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