TNF Alpha Antibody

//www.bio-rad-antibodies.com/human-tnf-alpha-antibody-ahp1212.html?RenderPageSpecific=1
TNF Alpha Antibody gallery image 1

Purchased this product? Why not be the first to submit a review and upload an image?

Enlarge
  • TNF Alpha Antibody thumbnail image 1
  • Goat anti Human TNF Alpha
  • Goat anti Human TNF Alpha:Biotin
(Rated 0.0 out of 5 based on 0 customer reviews)
  • Product Type
    Polyclonal Antibody
  • Isotype
    Polyclonal IgG
2 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    AHP1212E, FN, P *, WBdatasheet pdfdatasheet pdf0.1 mg
    AHP1212
    AHP1212BE, WBdatasheet pdfdatasheet pdf50 µg
    AHP1212B
    Summary
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    More Images
    References
    Reviews
    -
    • Goat anti Human TNF alpha antibody recognizes human Tumour Necrosis Factor alpha (TNF–alpha), a 17.4kDa multi-functional pro-inflammatory cytokine primarily secreted by macrophages, but also produced by T and B lymphocytes, activated monocytes and fibroblasts.

      TNF-alpha exists in both a transmembrane and mature soluble form and has many biological properties, including activation of transcription factor NF-kappaB, the induction of apoptosis in various tumour cell lines and the stimulation of cell differentiation and proliferation under certain conditions. TNF-alpha also plays a role in inflammation, septic shock, autoimmune disease and rheumatoid arthritis.
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution
      ELISA0.251.0ug/ml
      Functional Assays0.0120.014ug/ml
      Western Blotting0.10.2ug/ml
      Immunohistology - Paraffin(1)2.55.0ug/ml
      (1)
      This product heat-mediated antigen retrieval prior to staining paraffin sections. Citrate buffer, pH6.0, is recommended for this purpose.
    • Application NameYesNoMin DilutionMax Dilution
      ELISA0.251.0ug/ml
      Western Blotting0.10.2ug/ml

    • Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
    • Technical Advice
    • Technical Advice
    • Recommended Protocol
    • Recommended Protocol
    • ELISA
      This purified human TNFα antibody may be used in an indirect ELISA or as the capture reagent in a sandwich ELISA with our biotinylated TNFα antibody (AHP1212B) as the detection reagent and recombinant human TNFα (PHP051) as the standard
    • ELISA
      This biotinylated human TNFα antibody may be used in a direct ELISA or as the detection reagent in a sandwich ELISA with our purified human TNFα antibody (AHP1212) as the capture reagent and recombinant human TNFα (PHP051) as the standard.
    • Immunohistology
    • Immunohistology
    • Histology Positive Control Tissue
      Human Breast Carcinoma
    • Histology Positive Control Tissue
    • Immunofluorescence
    • Immunofluorescence
    • Western Blotting
    • Western Blotting
    • Instructions For Use
    • Instructions For Use

    Additional TNF Alpha Antibody Formats

    Formats Applications Sizes available
    TNF Alpha Antibody : Biotin E, WB 50 µg
    TNF Alpha Antibody : Purified E, FN, P *, WB 0.1 mg
    • Copyright © 2016 Bio-Rad

    Recommended Secondary Antibody

      DescriptionProduct CodePack SizeApplicationsList PriceQuantity
      Rabbit anti Goat IgG (Fc):FITCSTAR122F1 mgF
      STAR122F
      Rabbit anti Goat IgG (Fc):HRPSTAR122P1 mgC, E, WB
      STAR122P
      DescriptionProduct CodePack SizeApplicationsList PriceQuantity
      Streptavidin:APCSTAR119100 TestsF
      STAR119

      Recommended Negative Isotype Control

        Useful Reagents

          DescriptionProduct CodePack SizeApplicationsList PriceQuantity
          Antigen Retrieval Buffer, pH8.0BUF025C50 mlP
          BUF025C
          Antigen Retrieval Buffer, pH8.0BUF025A500 mlP
          BUF025A

          Recommended Positive Controls

            Histology Controls

              Human Breast Carcinoma

              References

              Write your review

              You may also be interested in...