TNF Alpha

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  • Recombinant Human TNF Alpha
  • Recombinant Human TNF Alpha
(Rated 0.0 out of 5 based on 0 customer reviews)
  • Product Type
    Recombinant Protein
1 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    PHP051AE, FN, WBdatasheet pdfdatasheet pdf10 µg
    PHP051A
    PHP051E, FN, WBdatasheet pdfdatasheet pdf50 µg
    PHP051
    Summary
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    More Images
    References
    Reviews
    -
    • PHP051 is an E. coli-derived recombinant protein corresponding to the C-terminal 157 amino acids of human Tumour Necrosis Factor Alpha (TNFa), a cytokine which exerts cytotoxic effects on a wide range of tunour cells.

      PHP051 has an ED50 of <0.05 ng/ml as determined by an assay measuring cytolysis of L929 cells in the presence of Actinomycin D.
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution
      ELISA0.20.4ng/well
      Functional Assays
      0.05Pack Size: 50 µg
      0.05ng/mlPack Size: 10 µg
      20.0ng/mlPack Size: 50 µg
      20ng/mlPack Size: 10 µg
      Western Blotting1.53.0ng/lane
    • Application NameYesNoMin DilutionMax Dilution
      ELISA0.20.4ng/well
      Functional Assays
      0.05Pack Size: 50 µg
      0.05ng/mlPack Size: 10 µg
      20.0ng/mlPack Size: 50 µg
      20ng/mlPack Size: 10 µg
      Western Blotting1.53.0ng/lane

    • Where this protein has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the protein for use in their own system using appropriate positive/negative controls.
    • Technical Advice
    • Technical Advice
    • Recommended Protocol
    • Recommended Protocol
    • ELISA
      This product may be used as a standard in ELISA with either AHP1212 or AHP1212B.
    • ELISA
      PHP051 may be used as a standard in a sandwich ELISA with AHP1212 as the capture reagent and AHP1212B as the detection reagent.
    • Immunohistology
    • Immunohistology
    • Histology Positive Control Tissue
    • Histology Positive Control Tissue
    • Immunofluorescence
    • Immunofluorescence
    • Western Blotting
      This product may be used as the positive control in Western Blotting applications with either AHP1212 or AHP1212B.
    • Western Blotting
      PHP051 may be used as a positive control in western blotting for either AHP1212 or AHP1212B.
    • Instructions For Use
    • Instructions For Use

    Additional TNF Alpha Formats

    Formats Applications Sizes available
    TNF Alpha : Rec. Protein E, FN, WB 10 µg | 50 µg
    • Copyright © 2016 Bio-Rad

    Recommended Secondary Antibody

      Recommended Negative Isotype Control

        Useful Reagents

          Recommended Positive Controls

            Histology Controls

              References

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