SLPI Antibody

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SLPI Antibody gallery image 1

Western blot analysis of whole cell lysate from Daudi, Burkitt's lymphoma derived cels probed with Rabbit anti Human SLP1 probed with Rabbit anti Human SLP1 (AHP1437) at 1(A) and 2(B) ug/ml

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SLPI Antibody gallery image 2

Formalin fixed, paraffin embedded mouse lung stained with Rabbit anti Human SLP1 (AHP1437) following heat mediated antigen retrieval using sodium citrate buffer (pH6.0)

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  • SLPI Antibody thumbnail image 1
  • SLPI Antibody thumbnail image 2
  • Rabbit anti SLPI
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  • Product Type
    Polyclonal Antibody
  • Isotype
    Polyclonal IgG
1 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    AHP1437P *, WBdatasheet pdfdatasheet pdf0.1 mg
    AHP1437
    Summary
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    More Images
    References
    Reviews
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    • Rabbit anti Human SLPI antibody recognises secretory leukocyte protease inhibitor (SLPI), a ~14 kDa non-glycosylated protein produced by secretory cells in the respiratory, genital and lacrimal glands, and by macrophages, B-cells and neutrophils.

      The functions of SLPI are diverse. The SLPI protein inhibits protease activity at sites of inflammation, protecting tissues against protease mediated damage and it possesses anti-microbial/anti-viral activity. In addition, SLPI has been reported to exhibit anti-inflammatory properties, regulating lipopolysaccharide induced activation of NF-kappa beta in peripheral blood monocytes.
    • Intended Use
    • Target Species
      Human
    • Species Cross-Reactivity
      Target SpeciesCross Reactivity
      Mouseyes
      N.B. Antibody reactivity and working conditions may vary between species.
    • Product Form
      Purified IgG - liquid
    • Reconstitution
    • Preparation
    • Antiserum Preparation
      Antisera to human SLPI were raised by repeated immunisation of rabbits with highly purified antigen. Purified IgG was prepared by affinity chromatography.
    • Preservative Stabilisers
      0.02%Sodium Azide
    • Immunogen
      A 17 amino acid peptide located near the centre of human SLPI.
    • Purity
    • Approx. Protein Concentrations
      IgG concentration 1.0 mg/ml
    • Reagents In The Kit
    • Preparing The Antibody
    • Test Principle
    • Buffer Solution
      Phosphate buffered saline
    • Storage
      Store at +4oC or at -20oC if preferred.

      This product should be stored undiluted.

      Storage in frost free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
    • Shelf Life
      18 months from date of despatch.
    • GO Terms
      serine-type endopeptidase inhibitor activity
      extracellular region
    • UniProt
    • Entrez Gene
    • Acknowledgements
    • Regulatory
      For research purposes only
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution
      Western Blotting1.02.0ug/ml
      Immunohistology - Paraffin(1)
      (1)
      This product requires antigen retrieval using heat treatment prior to staining of paraffin sections. Sodium citrate buffer pH 6.0 is recommended for this purpose.

    • Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
    • Technical Advice
    • Recommended Protocol
    • ELISA
    • Immunohistology
    • Histology Positive Control Tissue
    • Immunofluorescence
    • Western Blotting
      AHP1437 detects a band of approximately 14kDa in Daudi cell lysates.
    • Instructions For Use

    Additional SLPI Antibody Formats

    Formats Applications Sizes available
    SLPI Antibody : Purified P *, WB 0.1 mg
    • Copyright © 2016 Bio-Rad Antibodies (formerly AbD Serotec)

    Recommended Secondary Antibody

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      Recommended Negative Isotype Control

        Useful Reagents

          DescriptionProduct CodePack SizeApplicationsList PriceQuantity
          Antigen Retrieval Buffer, pH8.0BUF025C50 mlP
          BUF025C
          TidyBlot™ Western Blot Detection Reagent:HRPSTAR209P0.5 mlWB*
          STAR209P
          Antigen Retrieval Buffer, pH8.0BUF025A500 mlP
          BUF025A

          Recommended Positive Controls

            Histology Controls

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