SDF-1 Alpha Antibody

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SDF-1 Alpha Antibody gallery image 1

Formalin fixed, paraffin embedded human colorectal carcinoma stained with Rabbit anti Human SDF-1α (AHP794) following heat mediated antigen retrieval using sodium citrate buffer (pH6.0)

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SDF-1 Alpha Antibody gallery image 2

Formalin fixed, paraffin embedded human colorectal carcinoma stained with Rabbit anti Human SDF-1α (AHP794) following heat mediated antigen retrieval using sodium citrate buffer (pH6.0)

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  • Rabbit anti Human SDF-1 Alpha:Biotin
  • Rabbit anti Human SDF-1 Alpha
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  • Product Type
    Polyclonal Antibody
  • Isotype
    Polyclonal IgG
2 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    AHP794E, FN, P *, WBdatasheet pdfdatasheet pdf0.1 mg
    AHP794
    AHP794BE, WBdatasheet pdfdatasheet pdf50 µg
    AHP794B
    Summary
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    More Images
    References
    Reviews
    -
    • Rabbit anti Human SDF-1 alpha antibody recognizes human SDF-1 alpha, otherwise known as CXCL12a, a 68 amino acid stromal cell derived CXC chemokine, which arises from alternative splicing of the SDF-1 gene. The two isoforms, SDF-1 alpha and SDF-1 beta, share an identical amino acid sequence, except for an additional four residues in the C-Terminal region of the beta isoform.

      SDF-1 binds with high-affinity to the G protein–coupled receptor CXCR4 (fusin) and acts as a chemoattractant for T and B lymphocytes, monocytes and migratory neurons, and is a vital factor in haematopoiesis and angiogenesis. By competitively binding to CXCR4, SDF-1 acts as an inhibitor for the CXCR4-mediated entry of HIV-1 virus into target T-cells, and the SDF-1-CXCR4 interaction is important for the regulation of trafficking of normal and malignant stem cells.
    • Intended Use
    • Target Species
      Human
    • Product Form
      Purified IgG conjugated to Biotin - lyophilised
      Purified IgG - lyophilised
    • Reconstitution
      Reconstitute with 0.5ml sterile PBS containing 0.1% Bovine Serum Albumin.
      Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. Bio-Rad recommend that the vial is gently mixed after reconstitution. For long term storage the addition of 0.09% sodium azide is recommended.
      Reconstitute with 0.1ml distilled water. For long term storage the addition of 0.09% sodium azide is recommended. NB: For functional studies do not add azide.
    • Preparation
    • Antiserum Preparation
      Antisera to human SDF-1α were raised by repeated immunisations of rabbits with highly purified antigen. Purified IgG prepared by affinity chromatography.
    • Preservative Stabilisers
      None present
      None present
    • Immunogen
    • Purity
    • Approx. Protein Concentrations
      IgG concentration 0.1 mg/ml after reconstitution
      IgG concentration 1.0 mg/ml after reconstitution.
    • Reagents In The Kit
    • Preparing The Antibody
    • Test Principle
    • Buffer Solution
      Phosphate buffered saline
      Phosphate buffered saline
    • Storage
      Prior to reconstitution store at +4oC.
      After reconstitution store at -20oC.
      Storage in frost-free freezers is not recommended. This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
      Prior to reconstitution store at +4oC.
      After reconstitution store at -20oC.
      Storage in frost-free freezers is not recommended. This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
    • Shelf Life
      12 months from date of reconstitution.
      12 months from date of reconstitution.
    • GO Terms
      chemokine activity
      regulation of actin polymerization or depolymerization
      cell adhesion
      G-protein coupled receptor protein signaling pathway
      blood circulation
      extracellular space
      chemotaxis
      immune response
      response to virus
      positive regulation of monocyte chemotaxis
      cellular calcium ion homeostasis
      signal transducer activity
      growth factor activity
    • UniProt
    • Entrez Gene
    • Acknowledgements
    • Regulatory
      For research purposes only
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution
      ELISA0.251.0ug/ml
      Western Blotting0.10.2ug/ml
    • Application NameYesNoMin DilutionMax Dilution
      ELISA0.52.0ug/ml
      Functional Assays2.04.0ug/ml
      Western Blotting0.10.2ug/ml
      Immunohistology - Paraffin(1)0.5ug/ml
      (1)
      This product requires antigen retrieval using heat treatment prior to staining of paraffin sections.Sodium citrate buffer pH 6.0 is recommended for this purpose.

    • Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
    • Technical Advice
    • Technical Advice
    • Recommended Protocol
    • Recommended Protocol
    • ELISA
      This biotinylated human SDF-1α antibody may be used in a direct ELISA or as the detection reagent in a sandwich ELISA with our purified human SDF-1α antibody (AHP794) as the capture reagent and recombinant human SDF-1α (PHP122) as the standard.
    • ELISA
      This purified human SDF-1α antibody may be used in an indirect ELISA or as the capture reagent in a sandwich ELISA with our biotinylated human SDF-1α antibody (AHP794B) as the detection reagent and recombinant human SDF-1α (PHP122) as the standard
    • Immunohistology
    • Immunohistology
    • Histology Positive Control Tissue
    • Histology Positive Control Tissue
      Human colorectal adenocarcinoma
    • Immunofluorescence
    • Immunofluorescence
    • Western Blotting
      This antibody may be used in Western Blotting under either reducing or non-reducing conditions with recombinant human SDF-1α (PHP122) as the positive control.
    • Western Blotting
      This antibody may be used in Western Blotting under either reducing or non-reducing conditions with recombinant human SDF-1α (PHP122) as the positive control.
    • Instructions For Use
    • Instructions For Use

    Additional SDF-1 Alpha Antibody Formats

    Formats Applications Sizes available
    SDF-1 Alpha Antibody : Biotin E, WB 50 µg
    SDF-1 Alpha Antibody : Purified E, FN, P *, WB 0.1 mg
    • Copyright © 2016 Bio-Rad

    Recommended Secondary Antibody

      DescriptionProduct CodePack SizeApplicationsList PriceQuantity
      Streptavidin:APCSTAR119100 TestsF
      STAR119
      DescriptionProduct CodePack SizeApplicationsList PriceQuantity
      Goat anti Rabbit IgG (Fc):BiotinSTAR121B1 mgE, WB
      STAR121B
      Sheep anti Rabbit IgG:Biotin2AB02B1 mlC, E, P, WB
      2AB02B
      Sheep anti Rabbit IgG:DyLight®488STAR36D488GA0.1 mgF, IF
      STAR36D488GA
      Sheep anti Rabbit IgG:DyLight®549STAR36D549GA0.1 mgF, IF
      STAR36D549GA
      Sheep anti Rabbit IgG:DyLight®649STAR36D649GA0.1 mgF, IF
      STAR36D649GA
      Sheep anti Rabbit IgG:Dylight®800STAR36D800GA0.1 mgF, IF, WB
      STAR36D800GA
      Goat anti Rabbit IgG (Fc):FITCSTAR121F1 mgF
      STAR121F
      Sheep anti Rabbit IgG:FITCSTAR34B1 mgC, F
      STAR34B
      Goat anti Rabbit IgG (Fc):HRPSTAR121P1 mgE, WB
      STAR121P
      Goat anti Rabbit IgG (H/L):HRPSTAR124P1 mgC, E, WB
      STAR124P
      Sheep anti Rabbit IgG:HRP (Multi Adsorbed)STAR541 mgC, E, WB
      STAR54
      Sheep anti Rabbit IgG:RPESTAR35A1 mlF
      STAR35A

      Recommended Negative Isotype Control

        Useful Reagents

          DescriptionProduct CodePack SizeApplicationsList PriceQuantity
          Rabbit anti Human SDF-1 AlphaAHP7940.1 mgE, FN, P *, WB
          AHP794
          DescriptionProduct CodePack SizeApplicationsList PriceQuantity
          Antigen Retrieval Buffer, pH8.0BUF025C50 mlP
          BUF025C
          Rabbit anti Human SDF-1 Alpha:BiotinAHP794B50 µgE, WB
          AHP794B
          TidyBlot Western Blot Detection Reagent:HRPSTAR209P0.5 mlWB*
          STAR209P
          Antigen Retrieval Buffer, pH8.0BUF025A500 mlP
          BUF025A

          Recommended Positive Controls

            DescriptionProduct CodePack SizeApplicationsList PriceQuantity
            Recombinant Human SDF-1 AlphaPHP12210 µgE, FN, WB
            PHP122
            DescriptionProduct CodePack SizeApplicationsList PriceQuantity
            Recombinant Human SDF-1 AlphaPHP12210 µgE, FN, WB
            PHP122

            Histology Controls

              Human colorectal adenocarcinoma

              References

              Further Reading

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