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MAP1LC3A/B antibody

Rabbit anti Human MAP1LC3A/B (N-Terminal)

Product Type
Polyclonal Antibody
Isotype
Polyclonal IgG
Specificity
MAP1LC3A/B
Region
(N-TERMINAL)

Product Code Applications Pack Size List Price Your Price Qty
AHP2167T
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
IF WB 25 µg loader
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loader
AHP2167
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
IF WB 0.1 mg loader
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loader

Rabbit anti Human MAP1LC3A/B (N-Terminal) antibody specifically recognizes an epitope within the N-Terminal (NT) region of both MAP1LC3A (Microtubule-associated proteins 1A/1B light chain 3A/LC3A) and MAP1LC3B (Microtubule-associated proteins 1A/1B light chain 3B/LC3B), ubiquitin-like proteins and members of the MAP1LC3 family, which are widely used as reliable markers for the monitoring of autophagy.

LC3-I is the cytosolic form of LC3, which is converted into the active, membrane-bound form LC3-II, during the autophagy process. Tracking the level of conversion of LC3-I to LC3-II provides an indicator of autophagic activity, and levels of LC3-II in particular, correlate with the extent of autophagosome formation, due to its association with the autophagosome membrane.

Rabbit anti Human MAP1LC3A/B (N-Terminal) antibody recognizes both the LC3-I and LC3-II forms of MAP1LC3A and MAP1LC3B.

Target Species
Human
Species Cross-Reactivity
Target SpeciesCross Reactivity
Mouse
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Antiserum Preparation
Antiserum to human LC3A was raised by repeated immunisation of rabbits with highly purified antigen. Purified IgG was prepared by affinity chromatography.
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
Immunogen
Synthetic peptide sequence PSDRPFKQRRSFADC from the N-Terminal region of LC3A (NP_115903.1; NP_852610.1).
Approx. Protein Concentrations
IgG concentration 1.0mg/ml
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Immunofluorescence
Western Blotting 1/1000 1/5000
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Western Blotting
AHP2167 detects a band of approximately 14-15kDa corresponding to LC3-II, and a band of approximately 17kDa corresponding to LC3-I, in HeLa cell lysates.

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References for MAP1LC3A/B antibody

  1. Huang, L. et al. (2014) AKI after conditional and kidney-specific knockdown of stanniocalcin-1.
    J Am Soc Nephrol. 25: 2303-15.
  2. Gjyshi, O. et al. (2015) Kaposi's Sarcoma-Associated Herpesvirus Induces Nrf2 Activation in Latently Infected Endothelial Cells through SQSTM1 Phosphorylation and Interaction with Polyubiquitinated Keap1.
    J Virol. 89: 2268-86
  3. Girard, B.J. et al. (2015) Cytoplasmic PELP1 and ERRgamma Protect Human Mammary Epithelial Cells from Tam-Induced Cell Death.
    PLoS One. 10 (3): e0121206.

Synonyms
Atg8-LC3
RRID
AB_10698191
UniProt
Q9H492
Q9GZQ8
Q91VR7
Q9CQV6
Entrez Gene
Map1lc3a
Map1lc3b
MAP1LC3B
MAP1LC3A
GO Terms
GO:0000045 autophagic vacuole assembly
GO:0005515 protein binding
GO:0000421 autophagic vacuole membrane
GO:0005874 microtubule
GO:0012505 endomembrane system
GO:0031410 cytoplasmic vesicle
GO:0006914 autophagy
GO:0005829 cytosol
GO:0008429 phosphatidylethanolamine binding

AHP2167T

154035 1608

AHP2167

0915 155880

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