MAP1LC3A/B Antibody

//www.bio-rad-antibodies.com/human-map1lc3a-b-antibody-ahp2167.html?RenderPageSpecific=1
MAP1LC3A/B Antibody gallery image 1

HeLa lysate probed with Rabbit anti Human MAP1LC3A/B (N-terminal) (AHP2167) followed by Sheep anti Rabbit IgG:HRP (STAR54)

Enlarge
MAP1LC3A/B Antibody gallery image 2

Western blotting analysis of LC3 expression on Atg5 knockout and wild type cells using Rabbit anti Human MAP1LC3A/B (AHP2167). Image courtesy of Dr. R. Kapito, Stanford University

Enlarge
MAP1LC3A/B Antibody gallery image 3

Published customer image:
Rabbit anti Human MAP1LC3A/B antibody used for the detection of LC3-I and LC3-II in human mammary epitheial whole cell lysates by western blotting.
Image caption:
Cytoplasmic PELP1 and ERR? protect cells from Tam-induced autophagy. A, Cells in G1/G0 from cell cycle analysis of propidium iodide stained HMEC-LXSN and HMEC-Cyto cells treated with vehicle control or 1.0 µM Tam for 24 hours. A student’s T-Test was performed to determine statistical significance for changes in G1/G0 cell populations in response to Tam. P-values are given for the indicated comparisons. B, MTT assay of HMEC-hTERT cells treated with 0.5 or 1.0 µM Tam and ethanol control or 25 µM Z-VAD-FMK. A student’s T-Test was performed to determine statistical significance between control and Z-VAD-FMK treated cells in the presence of Tam. “ns” indicates no statistically significant differences were observed. C, Annexin V/7-AAD staining of HMEC-LXSN and HMEC-Cyto cells treated with 1.0 µM Tam for 24 hours. Student’s T-test was performed to determine statistical significance between Tam treated HMEC-LXSN and HMEC-cyto cells; p-values are shown for the indicated comparisons. D, Western blotting for LC3-I and LC3-II from HMEC-LXSN, HMEC-WT-1/WT-2, and HMEC-cyto lysates treated with ethanol control, 1 µM Tam, or 2 µM Tam for 48 hours. E, Western blot for LC3-II of whole cell lysates from HMECs expressing control or ERR? overexpression vector, treated with control or 0.5, 1, or 2 µM Tam for 24 hours. F, Western blot for LC3 from whole cell lysates of HMEC-LXSN and HMEC-cyto cells expressing either shControl or shERR?, treated with ethanol control or 1 µM Tam for 24 hours.

From: Girard BJ, Regan Anderson TM, Welch SL, Nicely J, Seewaldt VL, et al. (2015) Cytoplasmic PELP1 and ERRgamma Protect Human Mammary Epithelial Cells from Tam-Induced Cell Death.
PLoS ONE 10(3): e0121206.

Enlarge
MAP1LC3A/B Antibody gallery image 4

HeLA cells were fixed with 3% PFA and permeabilized with 0.3% Triton X-100. Nuclei were stained with 1.5 µg/ml DAPI. Immunofluorescence was carried out using AHP2167, a Rabbit polyclonal anti-Human Microtubule associated protein 1LC3A/B (MAP1LC3A/B) at 1/25. STAR36D488GA, Sheep anti-Rabbit DyLight®488 was then applied at 1/50. Image was visualized on the Bio-Rad ZOE Fluorescent Cell Imager at 60x magnification.

Enlarge
  • MAP1LC3A/B Antibody thumbnail image 1
  • MAP1LC3A/B Antibody thumbnail image 2
  • MAP1LC3A/B Antibody thumbnail image 3
  • MAP1LC3A/B Antibody thumbnail image 4
  • Rabbit anti Human MAP1LC3A/B (N-Terminal)
  • Rabbit anti Human MAP1LC3A/B (N-Terminal)
(Rated 0.0 out of 5 based on 0 customer reviews)
  • Product Type
    Polyclonal Antibody
  • Isotype
    Polyclonal IgG
1 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    AHP2167IF, WBdatasheet pdfdatasheet pdf0.1 mg
    AHP2167
    AHP2167TIF, WBdatasheet pdfdatasheet pdf25 µg
    AHP2167T
    Summary
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    More Images
    References
    Reviews
    -
    • Rabbit anti Human MAP1LC3A/B (N-Terminal) antibody specifically recognizes an epitope within the N-Terminal (NT) region of both MAP1LC3A (Microtubule-associated proteins 1A/1B light chain 3A/LC3A) and MAP1LC3B (Microtubule-associated proteins 1A/1B light chain 3B/LC3B), ubiquitin-like proteins and members of the MAP1LC3 family, which are widely used as reliable markers for the monitoring of autophagy.

      LC3-I is the cytosolic form of LC3, which is converted into the active, membrane-bound form LC3-II, during the autophagy process. Tracking the level of conversion of LC3-I to LC3-II provides an indicator of autophagic activity, and levels of LC3-II in particular, correlate with the extent of autophagosome formation, due to its association with the autophagosome membrane.

      Rabbit anti Human MAP1LC3A/B (N-Terminal) antibody recognizes both the LC3-I and LC3-II forms of MAP1LC3A and MAP1LC3B.
    • Intended Use
    • Target Species
      Human
    • Species Cross-Reactivity
      Target SpeciesCross Reactivity
      Mouseyes
      N.B. Antibody reactivity and working conditions may vary between species.
    • Product Form
      Purified IgG - liquid
      Purified IgG - liquid
    • Reconstitution
    • Preparation
    • Antiserum Preparation
      Antiserum to human LC3A was raised by repeated immunisation of rabbits with highly purified antigen. Purified IgG was prepared by affinity chromatography.
    • Preservative Stabilisers
      0.09% Sodium Azide (NaN3)
      0.09% Sodium Azide (NaN3)
    • Immunogen
      Synthetic peptide sequence PSDRPFKQRRSFADC from the N-Terminal region of LC3A (NP_115903.1; NP_852610.1).
    • Purity
    • Approx. Protein Concentrations
      IgG concentration 1.0mg/ml
      IgG concentration 1.0mg/ml
    • Reagents In The Kit
    • Preparing The Antibody
    • Test Principle
    • Buffer Solution
      Phosphate buffered saline
      Phosphate buffered saline
    • Storage
      Store at +4oC or at -20oC if preferred.
      Storage in frost-free freezers is not recommended.
      This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
      Store at +4oC or at -20oC if preferred.
      Storage in frost-free freezers is not recommended.
      This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
    • Shelf Life
      18 months from date of despatch.
      18 months from date of despatch.
    • GO Terms
      cytosol
      autophagic vacuole assembly
      phosphatidylethanolamine binding
      endomembrane system
      autophagy
      cytoplasmic vesicle
      autophagic vacuole membrane
      microtubule
      protein binding
    • UniProt
    • Entrez Gene
    • Acknowledgements
    • Regulatory
      For research purposes only
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution
      Immunofluorescence
      Western Blotting1/1001/250
    • Application NameYesNoMin DilutionMax Dilution
      Immunofluorescence
      Western Blotting1/1001/250

    • Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
    • Technical Advice
    • Technical Advice
    • Recommended Protocol
    • Recommended Protocol
    • ELISA
    • ELISA
    • Immunohistology
    • Immunohistology
    • Histology Positive Control Tissue
    • Histology Positive Control Tissue
    • Immunofluorescence
    • Immunofluorescence
    • Western Blotting
      AHP2167 detects a band of approximately 14-15kDa corresponding to LC3-II, and a band of approximately 17kDa corresponding to LC3-I, in HeLa cell lysates.
    • Western Blotting
      AHP2167T detects a band of approximately 14-15kDa corresponding to LC3-II, and a band of approximately 17kDa corresponding to LC3-I, in HeLa cell lysates.
    • Instructions For Use
    • Instructions For Use

    Additional MAP1LC3A/B Antibody Formats

    Formats Applications Sizes available
    MAP1LC3A/B Antibody : Purified IF, WB 0.1 mg | 25 µg
    • Copyright © 2016 Bio-Rad

    Recommended Secondary Antibody

      DescriptionProduct CodePack SizeApplicationsList PriceQuantity
      Goat anti Rabbit IgG (Fc):BiotinSTAR121B1 mgE, WB
      STAR121B
      Sheep anti Rabbit IgG:Biotin2AB02B1 mlC, E, P, WB
      2AB02B
      Sheep anti Rabbit IgG:DyLight®488STAR36D488GA0.1 mgF, IF
      STAR36D488GA
      Sheep anti Rabbit IgG:DyLight®549STAR36D549GA0.1 mgF, IF
      STAR36D549GA
      Sheep anti Rabbit IgG:DyLight®649STAR36D649GA0.1 mgF, IF
      STAR36D649GA
      Sheep anti Rabbit IgG:Dylight®800STAR36D800GA0.1 mgF, IF, WB
      STAR36D800GA
      Goat anti Rabbit IgG (Fc):FITCSTAR121F1 mgF
      STAR121F
      Sheep anti Rabbit IgG:FITCSTAR34B1 mgC, F
      STAR34B
      Goat anti Rabbit IgG (Fc):HRPSTAR121P1 mgE, WB
      STAR121P
      Goat anti Rabbit IgG (H/L):HRPSTAR124P1 mgC, E, WB
      STAR124P
      Sheep anti Rabbit IgG:HRP (Multi Adsorbed)STAR541 mgC, E, WB
      STAR54
      Sheep anti Rabbit IgG:RPESTAR35A1 mlF
      STAR35A
      DescriptionProduct CodePack SizeApplicationsList PriceQuantity
      Goat anti Rabbit IgG (Fc):BiotinSTAR121B1 mgE, WB
      STAR121B
      Sheep anti Rabbit IgG:Biotin2AB02B1 mlC, E, P, WB
      2AB02B
      Sheep anti Rabbit IgG:DyLight®488STAR36D488GA0.1 mgF, IF
      STAR36D488GA
      Sheep anti Rabbit IgG:DyLight®549STAR36D549GA0.1 mgF, IF
      STAR36D549GA
      Sheep anti Rabbit IgG:DyLight®649STAR36D649GA0.1 mgF, IF
      STAR36D649GA
      Sheep anti Rabbit IgG:Dylight®800STAR36D800GA0.1 mgF, IF, WB
      STAR36D800GA
      Goat anti Rabbit IgG (Fc):FITCSTAR121F1 mgF
      STAR121F
      Sheep anti Rabbit IgG:FITCSTAR34B1 mgC, F
      STAR34B
      Goat anti Rabbit IgG (Fc):HRPSTAR121P1 mgE, WB
      STAR121P
      Goat anti Rabbit IgG (H/L):HRPSTAR124P1 mgC, E, WB
      STAR124P
      Sheep anti Rabbit IgG:HRP (Multi Adsorbed)STAR541 mgC, E, WB
      STAR54
      Sheep anti Rabbit IgG:RPESTAR35A1 mlF
      STAR35A

      Recommended Negative Isotype Control

        Useful Reagents

          DescriptionProduct CodePack SizeApplicationsList PriceQuantity
          TidyBlot Western Blot Detection Reagent:HRPSTAR209P0.5 mlWB*
          STAR209P

          Recommended Positive Controls

            Histology Controls

              • Application NameReference Images
                Immunofluorescence
                Western Blotting

              References

              Write your review

              You may also be interested in...