IgM Antibody

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  • Goat F(ab')2 anti Human IgM:RPE
  • Goat F(ab')2 anti Human IgM
  • Goat F(ab')2 anti Human IgM:FITC
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  • Product Type
    Polyclonal Antibody
  • Isotype
    Polyclonal IgG
3 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    STAR146Edatasheet pdfdatasheet pdf0.5 mg
    STAR146
    STAR146FC, F, IFdatasheet pdfdatasheet pdf0.5 mg
    STAR146F
    STAR146PEFdatasheet pdfdatasheet pdf0.5 ml
    STAR146PE
    Summary
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    More Images
    References
    Reviews
    -
    • Goat F(ab')2 anti Human IgM antibody recognizes the heavy chain of human IgM and has been cross absorbed against human IgA and IgG. Goat F(ab')2 anti Human IgM antibody might cross react with IgM from other species.
    • Intended Use
    • Target Species
      Human
    • Product Form
      F(ab')2 fragment of IgG conjugated to R. Phycoerythrin (RPE) - liquid
      F(ab')2 fragments of purified IgG - liquid
      F(ab')2 fragments of purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid
    • Reconstitution
    • Preparation
    • Antiserum Preparation
      Antisera to human IgM were raised by repeated immunisation of goats with purified antigen. Purified IgG was prepared from whole serum by affinity chromatography.
      F(ab')2 fragments were prepared by pepsin digestion.
    • Preservative Stabilisers
      0.1% Sodium Azide (NaN3)
      Sucrose
      None present
      0.1% Sodium Azide (NaN3)
    • Immunogen
      Human IgM
    • Purity
    • Approx. Protein Concentrations
      F(ab')2 concentration 0.25mg/ml
      F(ab')2 concentration 0.5 mg/ml
      F(ab')2 concentration 0.5 mg/ml
    • Reagents In The Kit
    • Preparing The Antibody
    • Test Principle
    • Buffer Solution
      Phosphate buffered saline
      Borate buffered saline
      Phosphate buffered saline
    • Storage
      Store at +4oC.

      DO NOT FREEZE.

      This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.
      Store at -20oC only.
      Storage in frost-free freezers is not recommended.
      This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
      Store at +4oC or at -20oC if preferred.

      This product should be stored undiluted.

      Storage in frost free freezers is not recommended. This product is photosensitive and should be protected from light.

      Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
    • Shelf Life
      6 months from date of despatch.
      18 months from date of despatch.
      18 months from date of despatch.
    • GO Terms
      antigen binding
      integral to membrane
      membrane fraction
    • UniProt
    • Entrez Gene
    • Acknowledgements
    • Regulatory
      For research purposes only
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution
      Flow Cytometry1/201/30
    • Application NameYesNoMin DilutionMax Dilution
      ELISA
    • Application NameYesNoMin DilutionMax Dilution
      Flow CytometryNeat1/10
      ImmunofluorescenceNeat1/10
      Immunohistology - Frozen

    • Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
    • Technical Advice
    • Technical Advice
    • Technical Advice
    • Recommended Protocol
    • Recommended Protocol
    • Recommended Protocol
    • ELISA
    • ELISA
    • ELISA
    • Immunohistology
    • Immunohistology
    • Immunohistology
    • Histology Positive Control Tissue
    • Histology Positive Control Tissue
    • Histology Positive Control Tissue
    • Immunofluorescence
    • Immunofluorescence
    • Immunofluorescence
    • Western Blotting
    • Western Blotting
    • Western Blotting
    • Instructions For Use
    • Instructions For Use
    • Instructions For Use

    Additional IgM Antibody Formats

    Formats Applications Sizes available
    IgM Antibody : Purified E 0.5 mg
    IgM Antibody : RPE F 0.5 ml
    IgM Antibody : FITC C, F, IF 0.5 mg
    • Copyright © 2016 Bio-Rad

    Recommended Secondary Antibody

      DescriptionProduct CodePack SizeApplicationsList PriceQuantity
      Rabbit anti Goat IgG (Fc):FITCSTAR122F1 mgF
      STAR122F
      Rabbit anti Goat IgG (Fc):HRPSTAR122P1 mgC, E, WB
      STAR122P

      Recommended Negative Isotype Control

        Useful Reagents

          DescriptionProduct CodePack SizeApplicationsList PriceQuantity
          Human SeroblockBUF070B200 TestF
          BUF070B
          Human SeroblockBUF070A50 TestF
          BUF070A
          DescriptionProduct CodePack SizeApplicationsList PriceQuantity
          Human SeroblockBUF070B200 TestF
          BUF070B
          Human SeroblockBUF070A50 TestF
          BUF070A

          Recommended Positive Controls

            Histology Controls

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