IgG Antibody

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  • Goat F(ab')2 anti Human IgG:FITC
  • Goat F(ab')2 anti Human IgG
  • Goat F(ab')2 anti Human IgG:RPE
  • Goat F(ab')2 anti Human IgG:HRP
(Rated 0.0 out of 5 based on 0 customer reviews)
  • Product Type
    Polyclonal Antibody
  • Isotype
    Polyclonal IgG
4 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    STAR97PC, Edatasheet pdfdatasheet pdf0.6 mg
    STAR97P
    STAR97FC, Fdatasheet pdfdatasheet pdf0.7 mg
    STAR97F
    STAR97C, Edatasheet pdfdatasheet pdf0.8 mg
    STAR97
    STAR97PEFdatasheet pdfdatasheet pdf1 ml
    STAR97PE
    Summary
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    More Images
    References
    Reviews
    -
    • Goat F(ab')2 anti Human IgG antibody recognizes human IgG and demonstrates <1% cross-reactivity with other human immunoglobulin classes or subclasses.

      Goat F(ab')2 anti Human IgG antibody does not cross-react with Goat IgG F(c) or mouse, rat, bovine and horse serum proteins, as determined by immunoelectrophoresis.

      Goat F(ab')2 anti Human IgG antibody has been used for the detection of the antibody reponse to an endogenous retrovirus GAG matrix in patients with rheumatoid arthritis by ELISA (Nelson et al. 2014).
    • Intended Use
    • Target Species
      Human
    • Product Form
      F(ab')2 fragment of IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid
      F(ab')2 fragments of purified IgG - liquid
      F(ab')2 fragment of IgG conjugated to R. Phycoerythrin (RPE) - lyophilised
      F(ab')2 fragments of purified IgG conjugated to Horseradish Peroxidase (HRP) - liquid
    • Reconstitution
      Reconstitute with 1 ml distilled water
    • Preparation
    • Antiserum Preparation
      Antisera to human IgG were raised by repeated immunisation of goats with highly purified antigen. Purified IgG was prepared from whole serum by affinity chromatography. F(ab')2 fragments were prepared by pepsin digestion.
    • Preservative Stabilisers
      0.09%Sodium Azide
      1%Bovine Serum Albumin
      0.09%Sodium Azide
      0.09%Sodium Azide
      1%Bovine Serum Albumin
      5%Sucrose
      0.01% Thiomersal
      HRP Stabiliser (BUF052A)
    • Immunogen
      Human IgG
    • Purity
    • Approx. Protein Concentrations
      IgG concentration 0.7 mg/ml
      F(ab')2 concentration 0.8 mg/ml
      IgG concentration 0.6 mg/ml
    • Reagents In The Kit
    • Preparing The Antibody
    • Test Principle
    • Buffer Solution
      Phosphate buffered saline
      Phosphate buffered saline
      Phosphate buffered saline
      Phosphate buffered saline
    • Storage
      Store at +4oC or at -20oC if preferred.

      This product should be stored undiluted.

      Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light.

      Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
      Store at +4oC or at -20oC if preferred.

      This product should be stored undiluted.

      Storage in frost free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
      Store at +4oC.

      DO NOT FREEZE

      This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.
      Store at +4oC. DO NOT FREEZE.
      This product should be stored undiluted. Should this product contain a precipitate we recommend microcentrifugation before use.
    • Shelf Life
      18 months from date of despatch.
      18 months from date of despatch.
      12 months from date of reconstitution.
      18 months from date of despatch.
    • GO Terms
      complement activation, classical pathway
      membrane fraction
      protein binding
      innate immune response
      extracellular region
      immune response
      antigen binding
    • UniProt
    • Entrez Gene
    • Acknowledgements
    • Regulatory
      For research purposes only
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution
      Flow Cytometry1/501/100
      Immunohistology - Frozen
    • Application NameYesNoMin DilutionMax Dilution
      ELISA
      Immunohistology - Frozen
    • Application NameYesNoMin DilutionMax Dilution
      Flow CytometryNeat1/10
    • Application NameYesNoMin DilutionMax Dilution
      ELISA1/2000
      Immunohistology - Frozen

    • Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
    • Technical Advice
    • Technical Advice
    • Technical Advice
    • Technical Advice
    • Recommended Protocol
    • Recommended Protocol
    • Recommended Protocol
    • Recommended Protocol
    • ELISA
    • ELISA
    • ELISA
    • ELISA
    • Immunohistology
    • Immunohistology
    • Immunohistology
    • Immunohistology
    • Histology Positive Control Tissue
    • Histology Positive Control Tissue
    • Histology Positive Control Tissue
    • Histology Positive Control Tissue
    • Immunofluorescence
    • Immunofluorescence
    • Immunofluorescence
    • Immunofluorescence
    • Western Blotting
    • Western Blotting
    • Western Blotting
    • Western Blotting
    • Instructions For Use
    • Instructions For Use
    • Instructions For Use
    • Instructions For Use

    Additional IgG Antibody Formats

    Formats Applications Sizes available
    IgG Antibody : Purified C, E 0.8 mg
    IgG Antibody : FITC C, F 0.7 mg
    IgG Antibody : RPE F 1 ml
    IgG Antibody : HRP C, E 0.6 mg
    • Copyright © 2016 Bio-Rad

    Recommended Secondary Antibody

      DescriptionProduct CodePack SizeApplicationsList PriceQuantity
      Rabbit anti Goat IgG (Fc):FITCSTAR122F1 mgF
      STAR122F
      Rabbit anti Goat IgG (Fc):HRPSTAR122P1 mgC, E, WB
      STAR122P

      Recommended Negative Isotype Control

        Useful Reagents

          DescriptionProduct CodePack SizeApplicationsList PriceQuantity
          Human SeroblockBUF070B200 TestF
          BUF070B
          Human SeroblockBUF070A50 TestF
          BUF070A
          DescriptionProduct CodePack SizeApplicationsList PriceQuantity
          Human SeroblockBUF070B200 TestF
          BUF070B
          Human SeroblockBUF070A50 TestF
          BUF070A
          DescriptionProduct CodePack SizeApplicationsList PriceQuantity
          AbGUARD® HRP Stabilizer PlusBUF052A100 mlC, E, P, WB
          BUF052A
          TMB Signal+BUF054A100 mlE
          BUF054A
          TMB CoreBUF056A100 mlE
          BUF056A
          TMB Core PrestainedBUF057A100 mlE
          BUF057A
          TMB Core+BUF062A100 mlE
          BUF062A
          AbGUARD® HRP Stabilizer PlusBUF052C1000 mlC, E, P, WB
          BUF052C
          AbGUARD® HRP Stabilizer PlusBUF052B500 mlC, E, P, WB
          BUF052B

          Recommended Positive Controls

            Histology Controls

              References

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