IGF-I Antibody

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  • Rabbit anti Human Insulin-Like Growth Factor I
  • Rabbit anti Human Insulin-Like Growth Factor I:Biotin
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  • Product Type
    Polyclonal Antibody
  • Isotype
    Polyclonal IgG
2 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    AHP1281E, FN, P *, WBdatasheet pdfdatasheet pdf0.1 mg
    AHP1281
    AHP1281BE, WBdatasheet pdfdatasheet pdf50 µg
    AHP1281B
    Summary
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    More Images
    References
    Reviews
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    • Rabbit anti Human insulin-likegGrowth factor I antibody recognizes human IGF-I (Insulin-like Growth Factor I), a 70 amino acid secreted mitogenic polypeptide and member of the insulin gene family, produced primarily by the liver. IGF-I exhibits both autocrine and paracrine properties and has a widespread effect on cells including those of the bone, cartilage, muscle, nerves, skin, liver and lungs, playing a key role in cell proliferation and growth, inhibition of apoptosis and linked with tumour cell growth.

      IGF-I signals through binding to the type 1 insulin-like growth factor receptor (IGF-1R) and also to the insulin receptor (IR), resulting in the activation of the AKT signalling pathway. Production of IGF-I is stimulated by growth hormone (GH) and can be hindered by GH insensitivity, deficiency in GH receptors or a malfunction in the post GH receptor signalling pathway.
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution
      ELISA0.52.0ug/ml
      Functional Assays0.671.0ug/ml
      Western Blotting0.10.2ug/ml
      Immunohistology - Paraffin(1)0.25ug/ml
      (1)
      This antibody does not require antigen retrieval for use with formalin-fixed, paraffin-embedded sections.
    • Application NameYesNoMin DilutionMax Dilution
      ELISA0.251.0ug/ml
      Western Blotting0.10.2ug/ml

    • Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
    • Technical Advice
    • Technical Advice
    • Recommended Protocol
    • Recommended Protocol
    • ELISA
      This purified human IGF-I antibody may be used in an indirect ELISA or as the capture reagent in a sandwich ELISA with our biotinylated human IGF-I antibody (AHP1281B) as the detection reagent and recombinant human IGF-I (PHP177) as the standard.
    • ELISA
      This biotinylated human IGF-I antibody may be used in a direct ELISA or as the detection reagent in a sandwich ELISA with our purified human IGF-I antibody (AHP1281) as the capture reagent and recombinant human IGF-I (PHP177) as the standard.
    • Immunohistology
    • Immunohistology
    • Histology Positive Control Tissue
    • Histology Positive Control Tissue
    • Immunofluorescence
    • Immunofluorescence
    • Western Blotting
    • Western Blotting
    • Instructions For Use
    • Instructions For Use

    Additional IGF-I Antibody Formats

    Formats Applications Sizes available
    IGF-I Antibody : Purified E, FN, P *, WB 0.1 mg
    IGF-I Antibody : Biotin E, WB 50 µg
    • Copyright © 2016 Bio-Rad

    Recommended Secondary Antibody

      DescriptionProduct CodePack SizeApplicationsList PriceQuantity
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      DescriptionProduct CodePack SizeApplicationsList PriceQuantity
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      STAR119

      Recommended Negative Isotype Control

        Useful Reagents

          DescriptionProduct CodePack SizeApplicationsList PriceQuantity
          TidyBlot™ Western Blot Detection Reagent:HRPSTAR209P0.5 mlWB*
          STAR209P

          Recommended Positive Controls

            Histology Controls

              Further Reading

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