IgA Antibody

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  • Goat anti Human IgA Alpha Chain
  • Goat anti Human IgA:HRP
  • Goat anti Human IgA Alpha Chain:Alk. Phos.
(Rated 0.0 out of 5 based on 0 customer reviews)
  • Product Type
    Polyclonal Antibody
  • Isotype
    Polyclonal IgG
3 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    5104-2004C, E, WBdatasheet pdfdatasheet pdf1 mg
    5104-2004
    5104-2404C, E, WBdatasheet pdfdatasheet pdf1 mg
    5104-2404
    5104-2104C, E, WBdatasheet pdfdatasheet pdf1 mg
    5104-2104
    Summary
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    More Images
    References
    Reviews
    -
    • Goat anti Human IgA alpha chain antibody recognizes the alpha chain of human immunoglobulin A.

      Goat anti Human IgA alpha chain antibody has been cross-adsorbed to minimise cross-reactivity with other human immunoglobulin subclasses.
    • Intended Use
    • Target Species
      Human
    • Product Form
      Purified Ig - liquid
      Purified IgG conjugated to Horseradish Peroxidase (HRP) - lyophilised
      Purified IgG conjugated to Alkaline Phosphatase - liquid
    • Reconstitution
      Reconstitute with 1.0ml distilled water
      Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. Bio-Rad recommend that the vial is gently mixed after reconstitution.
    • Preparation
      Purified Ig prepared by immuno affinity chromatography
      Purified Ig prepared by immunoaffinity isolation on an agarose column
      Purified IgG prepared by affinity chromatography
    • Preservative Stabilisers
      0.09% Sodium Azide (NaN3)
      0.01%Gentamicin Sulphate
      1%Bovine Serum Albumin
      1mM Magnesium chloride
      0.1 mM Zinc chloride
      50% Glycerol
      1% Bovine Serum Albumin
      0.01% Sodium Azide (NaN3)
    • Immunogen
      Human IgA alpha chain
    • Purity
      IEP resulted in a single precipitin arc against anti-alkaline phosphatase (calf intestine), anti-goat serum, human IgA and human serum. No reaction was observed against human IgG F(c).
    • Approx. Protein Concentrations
      Ig concentration 1.0 mg/ml
      1.0 mg/ml after reconstitution
      IgG concentration 1.0 mg/ml
    • Reagents In The Kit
    • Preparing The Antibody
    • Test Principle
    • Buffer Solution
      Phosphate buffered saline
      Phosphate buffered saline
      TRIS buffered saline
    • Storage
      Store at +4oC or at -20oC if preferred.
      Storage in frost-free freezers is not recommended.
      This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
      Prior to reconstitution store at +4oC.
      After reconstitution store at -20oC.
      Storage in frost-free freezers is not recommended.
      +4ºC DO NOT FREEZE
    • Shelf Life
      18 months from date of despatch.
      12 months from date of reconstitution.
      18 months from date of despatch.
    • GO Terms
      protein-chromophore linkage
      protein binding
      immune response
      antigen binding
      membrane fraction
    • UniProt
    • Entrez Gene
    • Acknowledgements
    • Regulatory
      For research purposes only
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution
      ELISA
      Immunohistology - Frozen
      Western Blotting
    • Application NameYesNoMin DilutionMax Dilution
      ELISA1/10001/50000
      Immunohistology - Frozen1/5001/3000
      Western Blotting1/10001/5000
    • Application NameYesNoMin DilutionMax Dilution
      ELISA1/1
      Immunohistology - Frozen
      Western Blotting

    • Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using the appropriate negative/positive controls.
    • Technical Advice
    • Technical Advice
    • Technical Advice
    • Recommended Protocol
    • Recommended Protocol
    • Recommended Protocol
    • ELISA
    • ELISA
    • ELISA
    • Immunohistology
    • Immunohistology
    • Immunohistology
    • Histology Positive Control Tissue
    • Histology Positive Control Tissue
    • Histology Positive Control Tissue
    • Immunofluorescence
    • Immunofluorescence
    • Immunofluorescence
    • Western Blotting
    • Western Blotting
    • Western Blotting
    • Instructions For Use
    • Instructions For Use
    • Instructions For Use

    Additional IgA Antibody Formats

    Formats Applications Sizes available
    IgA Antibody : Alk. Phos. C, E, WB 1 mg
    IgA Antibody : HRP C, E, WB 1 mg
    IgA Antibody : Purified C, E, WB 1 mg
    • Copyright © 2016 Bio-Rad

    Recommended Secondary Antibody

      DescriptionProduct CodePack SizeApplicationsList PriceQuantity
      Rabbit anti Goat IgG (Fc):FITCSTAR122F1 mgF
      STAR122F
      Rabbit anti Goat IgG (Fc):HRPSTAR122P1 mgC, E, WB
      STAR122P

      Recommended Negative Isotype Control

        Useful Reagents

          DescriptionProduct CodePack SizeApplicationsList PriceQuantity
          AbGUARD® HRP Stabilizer PlusBUF052A100 mlC, E, P, WB
          BUF052A
          TMB Signal+BUF054A100 mlE
          BUF054A
          TMB CoreBUF056A100 mlE
          BUF056A
          TMB Core PrestainedBUF057A100 mlE
          BUF057A
          TMB Core+BUF062A100 mlE
          BUF062A
          AbGUARD® HRP Stabilizer PlusBUF052C1000 mlC, E, P, WB
          BUF052C
          AbGUARD® HRP Stabilizer PlusBUF052B500 mlC, E, P, WB
          BUF052B

          Recommended Positive Controls

            Histology Controls

              References

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