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GAPDH antibody | 4G5

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siRNA Validated
Anti GAPDH Antibody, clone 4G5 (PrecisionAb Monoclonal Antibody) thumbnail image 1 Anti GAPDH Antibody, clone 4G5 (PrecisionAb Monoclonal Antibody) thumbnail image 2 Anti GAPDH Antibody, clone 4G5 (PrecisionAb Monoclonal Antibody) thumbnail image 3 Anti GAPDH Antibody, clone 4G5 (PrecisionAb Monoclonal Antibody) thumbnail image 4
Anti GAPDH Antibody, clone 4G5 (PrecisionAb Monoclonal Antibody) gallery image 1
Western blot analysis of whole cell lysates probed with GAPDH antibody followed by detection with HRP conjugated Goat anti Mouse IgG (1/10,000, STAR207P) and visualized on the ChemiDoc MP with 2 second exposure. Arrow points to GAPDH (molecular weight 36 kDa).
Anti GAPDH Antibody, clone 4G5 (PrecisionAb Monoclonal Antibody) gallery image 2
Western blot analysis of whole cell lysates prepared from HEK293 separately transfected with two unique siRNA duplexes against GAPDH (1 & 2) or a scrambled siRNA (-) probed with Mouse anti GAPDH antibody (VMA00046) followed by detection with HRP conjugated Goat anti Mouse IgG (1/10,000, 1705047) and hFAB rhodamine anti-tubulin primary antibody (12004166) and visualized on the ChemiDoc MP with a 8 second (chemiluminescent channel) and 52 second exposure
Anti GAPDH Antibody, clone 4G5 (PrecisionAb Monoclonal Antibody) gallery image 3
Normalized signal intensity of GAPDH from whole cell lysates prepared from HEK293 separately transfected with two unique siRNA duplexes against GAPDH (1 & 2) or a scrambled siRNA (-). Signal intensity was normalized to the tubulin signal.
Anti GAPDH Antibody, clone 4G5 (PrecisionAb Monoclonal Antibody) gallery image 4
HeLa cells stained with Mouse anti GAPDH antibody (VMA00046) at a 1/250 dilution followed by detection with an Alexa Fluor 488 conjugated Goat anti Mouse IgG (H/L) antibody at a 1/800 dilution (green) and PureBlu™ DAPI (1351303) (blue) as a nuclear counterstain.

Mouse anti GAPDH

Product Type
PrecisionAb Monoclonal
Clone
4G5
Isotype
IgG1
Format
Purified
Specificity
GAPDH

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Product Code Applications Pack Size List Price Your Price Qty
VMA00046
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SDS Safety Datasheet SDS
IF WB 100 µl loader
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loader

Mouse anti Human GAPDH antibody recognizes glyceraldehyde-3-phosphate dehydrogenase (GAPDH), a 335 amino acid multifunctional protein whose main function is to catalyse the reversible oxidative phosphorylation of glyceraldehyde-3-phosphate, in conjunction with inorganic phosphate and nicotinamide adenine dinucleotide (NAD). This reaction is an important energy yielding step in carbohydrate metabolism.

GAPDH has also been shown to translocate to the nucleus under a variety of stressors, most of which are associated with oxidative stress, whereby it mediates cell death. A further report has shown that GAPDH binds to several proteins that are responsible for neurodegenerative diseases, such as amyloid precursor protein and huntingtin (Burke et al. 1996).

Mouse anti Human GAPDH antibody recognizes human GAPDH as a single band of ~36 kDa by western blotting in a variety of human cell line lysates under reducing conditions.		 PrecisionAb Antibodies

Target Species
Human
Western Blotting
Anti GAPDH antibody detects a band of approximately 36 kDa in HEK293 cell lysates.
Product Form
Purified IgG - liquid
Preparation
Mouse monoclonal antibody purified by affinity chromatography on Protein A from tissue culture supernatant.
Buffer Solution
Phosphate buffered saline.
Preservative Stabilisers
0.09% Sodium Azide (NaN3).
Immunogen
Human GAPDH from cardiac muscle
Approx. Protein Concentrations
IgG concentration 0.5 mg/ml.
Regulatory
For research purposes only.
Guarantee
12 months from date of despatch.
Acknowledgements
PrecisionAb is a trademark of Bio-Rad Laboratories.

Store undiluted at -20°C, avoiding repeated freeze thaw cycles.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Immunofluorescence
Western Blotting 1/1000
The PrecisionAb label is reserved for antibodies that meet the defined performance criteria within Bio-Rad's ongoing antibody validation programme. Click here to learn how we validate our PrecisionAb range. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependent on sample type.

Description Product Code Applications Pack Size List Price Your Price Quantity
Goat anti Mouse IgG (H/L):HRP STAR207P WB 2 ml loader
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loader
Description Goat anti Mouse IgG (H/L):HRP

Synonyms
Glyceraldehyde-3-Phosphate Dehydrogenase
UniProt
P04406
Entrez Gene
GAPDH
GO Terms
GO:0005515 protein binding
GO:0016020 membrane
GO:0004365 glyceraldehyde-3-phosphate dehydrogenase (phosphorylating) activity
GO:0005829 cytosol
GO:0005634 nucleus
GO:0006094 gluconeogenesis
GO:0006096 glycolysis
GO:0055114 oxidation-reduction process
GO:0050821 protein stabilization
GO:0035605 peptidyl-cysteine S-nitrosylase activity
GO:0035606 peptidyl-cysteine S-trans-nitrosylation
GO:0048471 perinuclear region of cytoplasm
GO:0051287 NAD binding
GO:0051402 neuron apoptosis

VMA00046

151149 160822

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