EGF Antibody

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Staining of formalin fixed, paraffin embedded normal human skin with Rabbit anti Human EGF (AHP767) following emzyme mediated antigen retrieval using proteinase K

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EGF Antibody gallery image 2

Staining of formalin fixed, paraffin embedded normal human skin with Rabbit anti Human EGF (AHP767) following emzyme mediated antigen retrieval using proteinase K

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  • EGF Antibody thumbnail image 1
  • EGF Antibody thumbnail image 2
  • Rabbit anti Human EGF
  • Rabbit anti Human EGF:Biotin
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  • Product Type
    Polyclonal Antibody
  • Isotype
    Polyclonal IgG
2 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    AHP767E, FN, P *, WBdatasheet pdfdatasheet pdf0.1 mg
    AHP767
    AHP767BE, WBdatasheet pdfdatasheet pdf50 µg
    AHP767B
    Summary
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    More Images
    References
    Reviews
    -
    • Rabbit anti human EGF recognizes human Epidermal Growth Factor, a potent stimulator and regulator of the proliferation of epidermal and epithelial cells, expressed by a variety of cells.

      The effects of EGF are initiated through tyrosine kinase activity, following the binding of EGF to the extracellular domain of the ~170 kDa EGF receptor (EGFR), which also binds other EGF-like ligands with high affinity, including TGF-a (transforming growth factor alpha), VGF (vaccinia virus growth factor) and Betacellulin, expressed in the pancreas, small intestine and by certain tumour cells (Seno et al. 1996). Receptor activation through EGF binding triggers several signal transduction pathways, including the JAK/STAT and P13K/AKT pathways.
    • Intended Use
    • Target Species
      Human
    • Product Form
      Purified IgG - lyophilised
      Purified IgG conjugated to Biotin - lyophilised
    • Reconstitution
      Reconstitute with 0.1ml distilled water.
      For long term storage the addition of 0.09% sodium azide is recommended.
      N.B. For functional studies do not add azide.
      Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. Bio-Rad recommend that the vial is gently mixed after reconstitution.
      Reconstitute with 0.5ml sterile PBS containing 0.1% Bovine Serum Albumin.
      Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. Bio-Rad recommend that the vial is gently mixed after reconstitution.
      For long term storage the addition of 0.09% sodium azide is recommended.
    • Preparation
    • Antiserum Preparation
      Antisera to human EGF were raised by repeated immunisations of rabbits with highly purified antigen.
      Purified IgG prepared by affinity chromatography.
    • Preservative Stabilisers
      None present.
      None present
    • Immunogen
    • Purity
    • Approx. Protein Concentrations
      IgG concentration 1.0mg/ml after reconstitution
      IgG concentration 0.1mg/ml after reconstitution.
    • Reagents In The Kit
    • Preparing The Antibody
    • Test Principle
    • Buffer Solution
      Phosphate buffered saline
      Phosphate buffered saline
    • Storage
      Prior to reconstitution store at +4oC.
      After reconstitution store at -20oC.
      Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
      Prior to reconstitution store at +4oC.
      After reconstitution store at -20oC.
      Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
    • Shelf Life
      12 months from date of reconstitution.
      12 months from date of reconstitution.
    • GO Terms
      transmembrane receptor protein tyrosine kinase activator activity
      platelet alpha granule lumen
      angiogenesis
      negative regulation of epidermal growth factor receptor signaling pathway
      platelet activation
      integral to membrane
      platelet degranulation
      plasma membrane
      regulation of calcium ion import
      growth factor activity
      positive regulation of MAP kinase activity
      epidermal growth factor receptor binding
      positive regulation of epidermal growth factor receptor activity
      regulation of protein localization at cell surface
      calcium ion binding
      DNA replication
      epidermal growth factor receptor signaling pathway
      negative regulation of secretion
      positive regulation of catenin import into nucleus
      positive regulation of mitosis
    • UniProt
    • Entrez Gene
    • Acknowledgements
    • Regulatory
      For research purposes only
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution
      ELISA0.52.0ug/ml
      Functional Assays<0.1ug/ml
      Western Blotting0.10.2ug/ml
      Immunohistology - Paraffin(1)5.0ug/ml
      (1)
      This product requires heat-mediated pre-treatment of paraffin sections prior to staining. Citrate buffer pH 6.0 is recommended for this purpose
    • Application NameYesNoMin DilutionMax Dilution
      ELISA0.251.0ug/ml
      Western Blotting0.10.2ug/ml

    • Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
    • Technical Advice
    • Technical Advice
    • Recommended Protocol
    • Recommended Protocol
    • ELISA
      This purified human EGF antibody may be used in an indirect ELISA or as the capture reagent in a sandwich ELISA with a biotinylated human EGF antibody (AHP767B) as the detection reagent and recombinant human EGF (PHP030A) as the standard.
    • ELISA
      This biotinylated human EGF antibody may be used in a direct ELISA or as the detection reagent in a sandwich ELISA with a purified human EGF antibody (AHP767) as the capture reagent and recombinant human EGF (PHP030A) as the standard.
    • Immunohistology
    • Immunohistology
    • Histology Positive Control Tissue
      Human kidney
    • Histology Positive Control Tissue
    • Immunofluorescence
    • Immunofluorescence
    • Western Blotting
      Rabbit anti human EGF may be used in Western Blotting applications under either reducing or non-reducing conditions with recombinant human EGF (PHP030A) as the positive control.
    • Western Blotting
      Rabbit anti human EGF may be used as in Western Blotting applications under either reducing or non-reducing conditions with recombinant human EGF (PHP030A) as the positive control.
    • Instructions For Use
    • Instructions For Use

    Additional EGF Antibody Formats

    Formats Applications Sizes available
    EGF Antibody : Purified E, FN, P *, WB 0.1 mg
    EGF Antibody : Biotin E, WB 50 µg
    • Copyright © 2016 Bio-Rad

    Recommended Secondary Antibody

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      Goat anti Rabbit IgG (Fc):FITCSTAR121F1 mgF
      STAR121F
      Sheep anti Rabbit IgG:FITCSTAR34B1 mgC, F
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      Goat anti Rabbit IgG (Fc):HRPSTAR121P1 mgE, WB
      STAR121P
      Goat anti Rabbit IgG (H/L):HRPSTAR124P1 mgC, E, WB
      STAR124P
      Sheep anti Rabbit IgG:HRP (Multi Adsorbed)STAR541 mgC, E, WB
      STAR54
      Sheep anti Rabbit IgG:RPESTAR35A1 mlF
      STAR35A
      DescriptionProduct CodePack SizeApplicationsList PriceQuantity
      Streptavidin:APCSTAR119100 TestsF
      STAR119

      Recommended Negative Isotype Control

        Useful Reagents

          DescriptionProduct CodePack SizeApplicationsList PriceQuantity
          Antigen Retrieval Buffer, pH8.0BUF025C50 mlP
          BUF025C
          TidyBlot Western Blot Detection Reagent:HRPSTAR209P0.5 mlWB*
          STAR209P
          Antigen Retrieval Buffer, pH8.0BUF025A500 mlP
          BUF025A

          Recommended Positive Controls

            Histology Controls

              Human kidney

              Further Reading

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