Collagen I Antibody

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Collagen I Antibody gallery image 1

Published customer image:
Rabbit anti Human collagen I antibody (2150-0020) used for the evaluation of collagen I expression in chondral repair tissue by immunohistochemistry on formalin fixed paraffin embedded tissue sections
Image caption:
Histological stains. Histological stains of the biopsies of cartilage and underlying bone that were taken from the site of the chondral allograft implantation on the trochlea at nine months following the repair (repair tissue) and from the trochlea of a cadaver (healthy tissue, for comparison). Scale bars = 500?μm.

From: Hoffman JK, Geraghty S, Protzman NM.
Articular cartilage repair using marrow stimulation augmented with a viable chondral allograft: 9-month postoperative histological evaluation.
Case Rep Orthop. 2015;2015:617365.

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Collagen I Antibody gallery image 2

Published customer image:
Rabbit anti Human collagen I antibody (2150-0020) used for the evaluation of collagen I expression in chondral repair tissue by immunohistochemistry on formalin fixed paraffin embedded tissue sections
Image caption:
Histological stains after marrow stimulation. Histological stains of cartilage repair tissue collected from 43-year-old patient 8.2 months after marrow stimulation surgery that required revision. Section outlined in blue in H&E stain is fibrocartilage, while the remaining tissue is primarily hyaline-like cartilage. Scale bars = 500?μm.

From: Hoffman JK, Geraghty S, Protzman NM.
Articular cartilage repair using marrow stimulation augmented with a viable chondral allograft: 9-month postoperative histological evaluation.
Case Rep Orthop. 2015;2015:617365.

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  • Rabbit anti Human Collagen I
(Rated 0.0 out of 5 based on 0 customer reviews)
  • Product Type
    Polyclonal Antibody
  • Isotype
    Polyclonal IgG
1 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    2150-0020C, E, P*datasheet pdfdatasheet pdf50 µg
    2150-0020
    Summary
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    More Images
    References
    Reviews
    -
    • Rabbit anti Human collagen I antibody recognizes human Collagen I, reacting with both native and heat denatured proteins, and has been cross-absorbed against Collagen types III, IV and V.
    • Intended Use
    • Target Species
      Human
    • Product Form
      Purified IgG - lyophilised
    • Reconstitution
      Reconstitute with 0.5ml distilled water
      Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. Bio-Rad recommend that the vial is gently mixed after reconstitution. For long term storage the addition of 1% BSA and 0.09% sodium azide is recommended.
    • Preparation
      Purified IgG prepared by affinity chromatography on immobilized Collagen type I
    • Preservative Stabilisers
      1% Dextran
      1% Mannitol
    • Immunogen
      Collagen type I from human placenta.
    • Purity
    • Approx. Protein Concentrations
    • Reagents In The Kit
    • Preparing The Antibody
    • Test Principle
    • Buffer Solution
      Phosphate buffered saline
    • Storage
      Prior to reconstitution store at +4oC.
      After reconstitution store at -20oC.
      Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody.
    • Shelf Life
      12 months from date of reconstitution.
    • UniProt
    • Entrez Gene
    • Acknowledgements
    • Regulatory
      For research purposes only
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution
      ELISA1/5001/3000
      Immunohistology - Frozen1/101/50
      Immunohistology - Paraffin(1)1/101/50
      (1)
      This product requires antigen retrieval using 1mg/ml pepsin in 0.5M acetic acid for 2 hours at 37oC and multiple buffer washes prior to staining of paraffin sections.

    • Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using the appropriate negative/positive controls.
    • Technical Advice
    • Recommended Protocol
    • ELISA
    • Immunohistology
    • Histology Positive Control Tissue
    • Immunofluorescence
    • Western Blotting
    • Instructions For Use

    Additional Collagen I Antibody Formats

    Formats Applications Sizes available
    Collagen I Antibody : Purified C, E, P* 50 µg
    • Copyright © 2017 Bio-Rad Antibodies (formerly AbD Serotec)

    Recommended Secondary Antibody

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      Recommended Negative Isotype Control

        Useful Reagents

          DescriptionProduct CodePack SizeApplicationsList PriceQuantity
          Antigen Retrieval Buffer, pH8.0BUF025C50 mlP
          BUF025C
          Antigen Retrieval Buffer, pH8.0BUF025A500 mlP
          BUF025A

          Recommended Positive Controls

            Histology Controls

              References

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