CD36 Antibody | SMØ

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CD36 Antibody | SMØ gallery image 1

Staining of human peripheral blood monocytes with Mouse anti Human CD36: Azide Free (MCA722XZ)

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CD36 Antibody | SMØ gallery image 2

Staining of human peripheral blood lymphocytes with Mouse anti Human CD36: FITC (MCA722F)

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CD36 Antibody | SMØ gallery image 3

Staining of human peripheral blood monocytes with Mouse anti Human CD36: Alexa Fluor® 647 (MCA722A647)

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CD36 Antibody | SMØ gallery image 4

Staining of human peripheral blood platelets with Mouse anti Human CD36 (MCA722)

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CD36 Antibody | SMØ gallery image 5

Staining of human peripheral blood platelets with Mouse anti Human CD36 (MCA722GA)

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CD36 Antibody | SMØ gallery image 6

Immunoperoxidase staining of human tonsil cryosection with Mouse anti Human CD36 antibody, clone SMø (MCA722) followed by the HISTAR detection system (STAR3000). Low power

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CD36 Antibody | SMØ gallery image 7

Immunoperoxidase staining of human tonsil cryosection with Mouse anti Human CD36 antibody, clone SMø (MCA722) followed by the HISTAR detection system (STAR3000). Medium power

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CD36 Antibody | SMØ gallery image 8

Immunoperoxidase staining of human tonsil cryosection with Mouse anti Human CD36 antibody, clone SMø (MCA722) followed by the HISTAR detection system (STAR3000). High power SMθ

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  • Mouse anti Human CD36
  • Mouse anti Human CD36:Preservative Free
  • Mouse anti Human CD36
  • Mouse anti Human CD36:FITC
  • Mouse anti Human CD36:FITC
  • Mouse anti Human CD36
  • Mouse anti Human CD36:Alexa Fluor® 647
(Rated 0.0 out of 5 based on 0 customer reviews)
  • Product Type
    Monoclonal Antibody
  • Clone
    SMØ
  • Isotype
    IgM
4 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    MCA722GAC, Fdatasheet pdfdatasheet pdf0.1 mg
    MCA722GA
    MCA722FFdatasheet pdfdatasheet pdf0.1 mg
    MCA722F
    MCA722C, Fdatasheet pdfdatasheet pdf0.2 mg
    MCA722
    MCA722XZC, F, FNdatasheet pdfdatasheet pdf1 mg
    MCA722XZ
    MCA722A647Fdatasheet pdfdatasheet pdf100 Tests/1ml
    MCA722A647
    MCA722TC, Fdatasheet pdfdatasheet pdf25 µg
    MCA722T
    MCA722FTFdatasheet pdfdatasheet pdf25 µg
    MCA722FT
    Summary
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    More Images
    References
    Reviews
    -
    • Mouse anti Human CD36 antibody, clone SMØ recognizes the CD36 antigen, an 88kD cell surface glycoprotein expressed by platelets, monocytes/macrophages and also by some microvascular endothelium and erythroid precursors.

      CD36 is the receptor for thrombospondin. When expressed by monocytes and macrophages, CD36 acts as a receptor for oxidized LDL and for apoptotic polymorphonuclear cells. Mouse anti Human CD36 antibody, clone SMØ partially inhibits collagen induced platelet aggregation and macrophage ingestion of apoptotic human neutrophils. It has been reported that this antibody may activate platelets as measured by fibrinogen binding, P-Selectin expression and Annexin V binding - for further details please contact our Technical Services team.

      Mouse anti Human CD36 antibody, clone SMØ is also reported to block interaction of oxidised low-density lipoprotein (OXLDL) with CD36 (Sugihara et al.1992).
    • Intended Use
    • Target Species
      Human
    • Product Form
      Purified IgM - liquid
      Purified IgM - liquid
      Purified IgM - liquid
      Purified IgM conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid
      Purified IgM conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid
      Purified IgM - liquid
      Purified IgM conjugated to Alexa Fluor® 647 - liquid
    • Reconstitution
    • Preparation
      Purified IgM prepared by ammonium sulphate precipitation from tissue culture supernatant
      Purified IgM prepared by ammonium sulphate precipitation from tissue culture supernatant
      Purified IgM prepared by ammonium sulphate precipitation from tissue culture supernatant
      Purified IgM prepared by ammonium sulphate precipitation from tissue culture supernatant
      Purified IgM prepared by ammonium sulphate precipitation from tissue culture supernatant
      Purified IgM prepared by ammonium sulphate precipitation from tissue culture supernatant
      Purified IgM prepared by ammonium sulphate precipitation from tissue culture supernatant
    • Preservative Stabilisers
      0.09%Sodium Azide
      None present
      0.09%Sodium Azide
      0.09%Sodium Azide
      1%Bovine Serum Albumin
      0.09%Sodium Azide
      1%Bovine Serum Albumin
      0.09%Sodium Azide
      0.09%Sodium Azide
      1%Bovine Serum Albumin
    • Immunogen
      Human tonsil cells and peripheral blood mononuclear cells.
    • Purity
    • Approx. Protein Concentrations
      IgM concentration 1 mg/ml
      IgM concentration 1 mg/ml
      IgM concentration 1 mg/ml
      IgM concentration 0.1 mg/ml
      IgM concentration 0.1 mg/ml
      IgM concentration 1 mg/ml
      IgM concentration 0.05 mg/ml
    • Reagents In The Kit
    • Preparing The Antibody
    • Test Principle
    • Buffer Solution
      Phosphate buffered saline
      Phosphate buffered saline
      Phosphate buffered saline
      Phosphate buffered saline
      Phosphate buffered saline
      Phosphate buffered saline
      Phosphate buffered saline
    • Fusion Partners
      Spleen cells from immunised mice were fused with cells of the NSI mouse myeloma line.
    • Storage
      Store at +4oC.

      DO NOT FREEZE.

      This product should be stored undiluted. Should this product contain a precipitate we recommend microcentrifugation before use.
      Store at -20o only.

      This product should be stored undiluted.
      Avoid repeated freezing and thawing as this may denature the protein.

      Storage in frost free freezers is not recommended. Should this product contain a precipitate we recommend microcentrifugation before use.
      Store at +4oC.

      DO NOT FREEZE.

      This product should be stored undiluted. Should this product contain a precipitate we recommend microcentrifugation before use.
      Store at +4oC.

      DO NOT FREEZE.

      This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.
      Store at +4oC.

      DO NOT FREEZE.

      This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.
      Store at +4oC.

      DO NOT FREEZE.

      This product should be stored undiluted. Should this product contain a precipitate we recommend microcentrifugation before use.
      Store at +4oC.

      DO NOT FREEZE.

      This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.
    • Shelf Life
      18 months from date of despatch.
      18 months from date of despatch.
      18 months from date of despatch.
      18 months from date of despatch.
      18 months from date of despatch.
      18 months from date of despatch.
      18 months from date of despatch.
    • UniProt
    • Entrez Gene
    • Acknowledgements
      This product is provided under an intellectual property licence from Life Technologies Corporation. The transfer of this product is contingent on the buyer using the purchase product solely in research, excluding contract research or any fee for service research, and the buyer must not sell or otherwise transfer this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; (c) manufacturing or quality assurance or quality control, or (d) resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad CA 92008 USA or outlicensing@thermofisher.com
    • Regulatory
      For research purposes only
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution
      Flow Cytometry
      1/50 Pack Size: 0.2 mg, 25 µg
      1/10 Pack Size: 0.1 mg
      1/100Pack Size: 0.2 mg, 25 µg
      1/20Pack Size: 0.1 mg
      Immunohistology - Frozen
      Immunoprecipitation
      Western Blotting
    • Application NameYesNoMin DilutionMax Dilution
      Flow Cytometry1/251/50
      Functional Assays
      Immunohistology - Frozen
      Immunoprecipitation
      Western Blotting
    • Application NameYesNoMin DilutionMax Dilution
      Flow Cytometry
      1/50 Pack Size: 0.2 mg, 25 µg
      1/10 Pack Size: 0.1 mg
      1/100Pack Size: 0.2 mg, 25 µg
      1/20Pack Size: 0.1 mg
      Immunohistology - Frozen
      Immunoprecipitation
      Western Blotting
    • Application NameYesNoMin DilutionMax Dilution
      Flow CytometryNeat1/5
    • Application NameYesNoMin DilutionMax Dilution
      Flow CytometryNeat1/5
    • Application NameYesNoMin DilutionMax Dilution
      Flow Cytometry
      1/50 Pack Size: 0.2 mg, 25 µg
      1/10 Pack Size: 0.1 mg
      1/100Pack Size: 0.2 mg, 25 µg
      1/20Pack Size: 0.1 mg
      Immunohistology - Frozen
      Immunoprecipitation
      Western Blotting
    • Application NameYesNoMin DilutionMax Dilution
      Flow Cytometry1/51/10

    • Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
    • Technical Advice
    • Technical Advice
    • Technical Advice
    • Technical Advice
    • Technical Advice
    • Technical Advice
    • Technical Advice
    • Recommended Protocol
    • Recommended Protocol
    • Recommended Protocol
    • Recommended Protocol
    • Recommended Protocol
    • Recommended Protocol
    • Recommended Protocol
    • Flow Cytometry
      Use 10ul of the suggested working dilution to label 106 cells or 100ul whole blood.
    • ELISA
    • ELISA
    • ELISA
    • ELISA
    • ELISA
    • ELISA
    • ELISA
    • Immunohistology
    • Immunohistology
    • Immunohistology
    • Immunohistology
    • Immunohistology
    • Immunohistology
    • Immunohistology
    • Histology Positive Control Tissue
    • Histology Positive Control Tissue
    • Histology Positive Control Tissue
    • Histology Positive Control Tissue
    • Histology Positive Control Tissue
    • Histology Positive Control Tissue
    • Histology Positive Control Tissue
    • Immunofluorescence
    • Immunofluorescence
    • Immunofluorescence
    • Immunofluorescence
    • Immunofluorescence
    • Immunofluorescence
    • Immunofluorescence
    • Western Blotting
    • Western Blotting
    • Western Blotting
    • Western Blotting
    • Western Blotting
    • Western Blotting
    • Western Blotting
    • Instructions For Use
    • Instructions For Use
    • Instructions For Use
    • Instructions For Use
    • Instructions For Use
    • Instructions For Use
    • Instructions For Use

    Additional CD36 Antibody Formats

    Formats Clone Applications Sizes available
    CD36 Antibody : Purified SMØ C, F 25 µg | 0.1 mg | 0.2 mg
    CD36 Antibody : FITC SMØ F 25 µg | 0.1 mg
    CD36 Antibody : Alexa Fluor® 647 SMØ F 100 Tests/1ml
    CD36 Antibody : Preservative Free SMØ C, F, FN 1 mg
    • Copyright © 2016 Bio-Rad

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        DescriptionProduct CodePack SizeApplicationsList PriceQuantity
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        DescriptionProduct CodePack SizeApplicationsList PriceQuantity
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          DescriptionProduct CodePack SizeApplicationsList PriceQuantity
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          Recommended Positive Controls

            Histology Controls

              • Application NameReference Images
                Functional Assays

              References

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