Caspase-9 Antibody

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Caspase-9 Antibody gallery image 1

Western blot analysis of whole cell lysates from HeLa cells probed with Rabbit anti Human caspase-9 (AHP492)

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Caspase-9 Antibody gallery image 2

Immunocytochemical staining for H562 cells with Rabbit anti Human caspase-9 (AHP492)

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  • Rabbit anti Human Caspase-9 (aa299-318)
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  • Product Type
    Polyclonal Antibody
  • Isotype
    Polyclonal IgG
1 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    AHP492WBdatasheet pdfdatasheet pdf0.1 mg
    AHP492
    Summary
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    More Images
    References
    Reviews
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    • Caspase-9 (also known as ICE-LAP6, McL6 and Apaf-3) plays a central role in programmed cell death.

      Following activation (induced by the binding of Apaf-1), caspase-9 cleaves and activates caspase-3.

      AHP492 recognises the 46kD caspase-9 in Western blots.
    • Intended Use
    • Target Species
      Human
    • Product Form
      Purified IgG - liquid
    • Reconstitution
    • Preparation
    • Antiserum Preparation
      Antisera to human caspase-9 were raised by repeated immunisation of rabbits with highly purified antigen. Purified IgG prepared by affinity chromatography.
    • Preservative Stabilisers
      0.02%Sodium Azide
    • Immunogen
      Synthetic peptide corresponding to amino acids 299-318 of human caspase-9
    • Purity
    • Approx. Protein Concentrations
      IgG concentration 0.5 mg/ml
    • Reagents In The Kit
    • Preparing The Antibody
    • Test Principle
    • Buffer Solution
      Phosphate buffered saline
    • Storage
      Store at +4oC or at -20oC if preferred.

      This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
    • Shelf Life
      18 months from date of despatch.
    • GO Terms
      nerve growth factor receptor signaling pathway
      protein binding
      cytosol
      activation of caspase activity by cytochrome c
      induction of apoptosis by intracellular signals
      proteolysis
      enzyme activator activity
      cysteine-type endopeptidase activity
      phosphatidylinositol-mediated signaling
    • UniProt
    • Entrez Gene
    • Acknowledgements
    • Regulatory
      For research purposes only
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution
      Western Blotting1/10001/2000

    • Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
    • Technical Advice
    • Recommended Protocol
    • ELISA
    • Immunohistology
    • Histology Positive Control Tissue
    • Immunofluorescence
    • Western Blotting
    • Instructions For Use

    Additional Caspase-9 Antibody Formats

    Formats Applications Sizes available
    Caspase-9 Antibody : Purified WB 0.1 mg
    • Copyright © 2016 Bio-Rad

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      Recommended Negative Isotype Control

        Useful Reagents

          DescriptionProduct CodePack SizeApplicationsList PriceQuantity
          TidyBlot Western Blot Detection Reagent:HRPSTAR209P0.5 mlWB*
          STAR209P

          Recommended Positive Controls

            Histology Controls

              Further Reading

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