Caspase-9 (Active) Antibody

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Caspase-9 (Active) Antibody gallery image 1

Western blot analysis of Jurkat cell lysates from untreated (lane 1) and etoposide-treated (lane 2) cells probed with Rabbit anti caspase-9 (active) antibody

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  • Rabbit anti Caspase-9 (Active)
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  • Product Type
    Polyclonal Antibody
  • Isotype
    Polyclonal IgG
1 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    AHP2288IP, WBdatasheet pdfdatasheet pdf50 µg
    AHP2288
    Summary
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    More Images
    References
    Reviews
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    • Rabbit anti caspase-9 antibody recognizes caspase-9 also known as apoptotic protease-activating factor 3 (APAF-3). Caspase-9 as the name implies is a member of the cysteine-aspartic acid protease family.

      The active form of caspase-9 is generated via cleavage of the pro-caspase-9 protein into two fragments, known as the large and the small fragment. The trigger for caspase-9 cleavage is the binding of cytochrome c to pro-caspase-9, which is released upon receiving apoptotic stimuli.

      Caspase-9 is an effector caspase that plays a role in the mediation of mitochondrial apoptosis. Active caspase-9 cleaves pro-caspases 3 and 7 into their active forms. Caspase activity can be inhibited by c-IAP1, c-IAP2, XIAP and other members of the inhibitor of apoptosis protein family.
    • Intended Use
    • Target Species
      Human
    • Product Form
      Purified IgG - liquid
    • Reconstitution
    • Preparation
    • Preservative Stabilisers
      50% Glycerol
      0.5% Bovine serum Albumin
      0.02% Thiomersal
    • Immunogen
      Synthetic peptide mapping to the N-terminus adjacent to Asp330 of human caspase-9
    • Purity
    • Approx. Protein Concentrations
      IgG concentration 0.2 mg/ml
    • Reagents In The Kit
    • Preparing The Antibody
    • Test Principle
    • Buffer Solution
      Phosphate buffered saline
    • Storage
      Store at -20oC
      Storage in frost-free freezers is not recommended.
      This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
    • Shelf Life
      12 months from date of despatch
    • GO Terms
      activation of caspase activity by cytochrome c
      nerve growth factor receptor signaling pathway
      proteolysis
      protein binding
      phosphatidylinositol-mediated signaling
      enzyme activator activity
      cytosol
      induction of apoptosis by intracellular signals
      cysteine-type endopeptidase activity
    • UniProt
    • Entrez Gene
    • Acknowledgements
    • Regulatory
      For research purposes only
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution
      Immunoprecipitation1020 ug/ml
      Western Blotting0.54.0 ug/ml

    • Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
    • Technical Advice
    • Recommended Protocol
    • ELISA
    • Immunohistology
    • Histology Positive Control Tissue
    • Immunofluorescence
    • Western Blotting
      Rabbit anti caspase-9 recognizes the cleaved form of caspase 9 (approximately 37kDa) in Jurkat cells treated with etoposide
    • Instructions For Use

    Additional Caspase-9 (Active) Antibody Formats

    Formats Applications Sizes available
    Caspase-9 (Active) Antibody : Purified IP, WB 50 µg
    • Copyright © 2016 Bio-Rad

    Recommended Secondary Antibody

      DescriptionProduct CodePack SizeApplicationsList PriceQuantity
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      STAR36D800GA
      Goat anti Rabbit IgG (Fc):FITCSTAR121F1 mgF
      STAR121F
      Sheep anti Rabbit IgG:FITCSTAR34B1 mgC, F
      STAR34B
      Goat anti Rabbit IgG (Fc):HRPSTAR121P1 mgE, WB
      STAR121P
      Goat anti Rabbit IgG (H/L):HRPSTAR124P1 mgC, E, WB
      STAR124P
      Sheep anti Rabbit IgG:HRP (Multi Adsorbed)STAR541 mgC, E, WB
      STAR54
      Sheep anti Rabbit IgG:RPESTAR35A1 mlF
      STAR35A

      Recommended Negative Isotype Control

        Useful Reagents

          Recommended Positive Controls

            Histology Controls

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