BMP-2 Antibody

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BMP-2 Antibody gallery image 1

Staining of formalin fixed, paraffin embedded human colo-rectal adenocarcinoma with Rabbit anti Human BMP-2 (AHP960)

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BMP-2 Antibody gallery image 2

Staining of formalin fixed, paraffin embedded human colo-rectal adenocarcinoma with Rabbit anti Human BMP-2 (AHP960)

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BMP-2 Antibody gallery image 3

U-2 osteosarcoma cells stained with Rabbit anti Human BMP-2 (AHP960) green, tubulin, red and nuclei, blue

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  • BMP-2 Antibody thumbnail image 1
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  • Rabbit anti Human BMP-2:Biotin
  • Rabbit anti Human BMP-2
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  • Product Type
    Polyclonal Antibody
  • Isotype
    Polyclonal IgG
2 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    AHP960BE, WBdatasheet pdfdatasheet pdf50 µg
    AHP960B
    AHP960E, IF, P *, WBdatasheet pdfdatasheet pdf50 µg
    AHP960
    Summary
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    More Images
    References
    Reviews
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    • Rabbit anti Human BMP-2 antibody recognizes the disulphide-linked homodimeric cysteine knot protein known as human Bone Morphogenetic Protein 2 (BMP-2/BMP-2a), full-length 396 amino acids. BMP-2 is a member of the Transforming Growth Factor beta (TGF-B) superfamily and one of a growing number of osteogenic proteins shown to induce bone and cartilage formation and to play an important role in developmental processes, including cell proliferation, differentiation, apoptosis and morphogenesis.

      BMPs act through binding with a receptor complex consisting of type I and type II serine/threonine kinases, resulting ultimately in the activation of the Smad protein and mitogen-activated protein kinase (MAPK) signaling pathways. Several antagonist proteins, including, noggin, chordin, gremlin and follistatin, are responsible for modulating the signaling effects of BMPs, through the binding and blocking of receptor ligands, thereby preventing activation.

      The realization that BMP-2 was involved in the stimulation of bone formation emerged from research in spinal surgery, following the discovery that the healing of bones was directed by proteins contained within the bone matrix itself.
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution
      ELISA0.25 ug/ml1.0 ug/ml
      Western Blotting0.1 ug/ml0.2 ug/ml
    • Application NameYesNoMin DilutionMax Dilution
      ELISA0.55.0ug/ml
      Immunofluorescence
      Western Blotting0.11.0ug/ml
      Immunohistology - Paraffin(1)0.25ug/ml
      (1)
      This product requires antigen retrieval using heat treatment prior to staining of paraffin sections. Sodium citrate buffer pH 6.0 is recommended for this purpose.

    • Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
    • Technical Advice
    • Technical Advice
    • Recommended Protocol
    • Recommended Protocol
    • ELISA
      This biotinylated human BMP-2 antibody may be used in a direct ELISA or as the detection reagent in a sandwich ELISA with our purified human BMP-2 antibody (AHP960) as the capture reagent.
    • ELISA
      This purified human BMP-2antibody may be used in an indirect ELISA or as the capture reagent in a sandwich ELISA with our biotinylated human BMP-2 antibody (AHP960B) as the detection reagent.
    • Immunohistology
    • Immunohistology
    • Histology Positive Control Tissue
    • Histology Positive Control Tissue
    • Immunofluorescence
    • Immunofluorescence
    • Western Blotting
      This antibody may be used in Western Blotting under either reducing or non-reducing conditions.
    • Western Blotting
      This antibody may be used in Western Blotting under either reducing or non-reducing conditions.
    • Instructions For Use
    • Instructions For Use

    Additional BMP-2 Antibody Formats

    Formats Applications Sizes available
    BMP-2 Antibody : Biotin E, WB 50 µg
    BMP-2 Antibody : Purified E, IF, P *, WB 50 µg
    • Copyright © 2016 Bio-Rad

    Recommended Secondary Antibody

      DescriptionProduct CodePack SizeApplicationsList PriceQuantity
      Streptavidin:APCSTAR119100 TestsF
      STAR119
      DescriptionProduct CodePack SizeApplicationsList PriceQuantity
      Goat anti Rabbit IgG (Fc):BiotinSTAR121B1 mgE, WB
      STAR121B
      Sheep anti Rabbit IgG:Biotin2AB02B1 mlC, E, P, WB
      2AB02B
      Sheep anti Rabbit IgG:DyLight®488STAR36D488GA0.1 mgF, IF
      STAR36D488GA
      Sheep anti Rabbit IgG:DyLight®549STAR36D549GA0.1 mgF, IF
      STAR36D549GA
      Sheep anti Rabbit IgG:DyLight®649STAR36D649GA0.1 mgF, IF
      STAR36D649GA
      Sheep anti Rabbit IgG:Dylight®800STAR36D800GA0.1 mgF, IF, WB
      STAR36D800GA
      Goat anti Rabbit IgG (Fc):FITCSTAR121F1 mgF
      STAR121F
      Sheep anti Rabbit IgG:FITCSTAR34B1 mgC, F
      STAR34B
      Goat anti Rabbit IgG (Fc):HRPSTAR121P1 mgE, WB
      STAR121P
      Goat anti Rabbit IgG (H/L):HRPSTAR124P1 mgC, E, WB
      STAR124P
      Sheep anti Rabbit IgG:HRP (Multi Adsorbed)STAR541 mgC, E, WB
      STAR54
      Sheep anti Rabbit IgG:RPESTAR35A1 mlF
      STAR35A

      Recommended Negative Isotype Control

        Useful Reagents

          DescriptionProduct CodePack SizeApplicationsList PriceQuantity
          Antigen Retrieval Buffer, pH8.0BUF025C50 mlP
          BUF025C
          TidyBlot Western Blot Detection Reagent:HRPSTAR209P0.5 mlWB*
          STAR209P
          Antigen Retrieval Buffer, pH8.0BUF025A500 mlP
          BUF025A

          Recommended Positive Controls

            Histology Controls

              References

              Further Reading

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