Rat anti Horse CD2 antibody, clone Mac288 recognizes equine CD2, a 347 amino acid, single pass type-1 transmembrane glycoprotein containing single Ig-like C2-type and Ig-like V-type domains.
Equine CD2 is expressed by a large proportion of peripheral blood lymphocytes, most T-cells and few B-cells, it is not present on granulocytes. Immunohistochemical staining shows expression in most T cells of lymph node paracortex and occasional staining of lymphocytes in the follicular areas. In the thymus, rat anti horse CD2 antibody, clone Mac288 stains the majority of mature thymocytes in the medulla and at least half of the immature thymocytes in the cortex (Kydd et al. 1994). Rat anti Horse CD2 antibody, clone Mac288 recognizes CD2 in Haflinger (Üner et al. 2013) and Lusitano horse breeds (Ferreira-Dias et al. 2005). Rat anti Horse CD2 antibody, clone Mac288 is a reliable pan T-cell marker for analysis of cell mediated immunity in horses infected with equine herpes virus by multi-parameter flow cytometry (Platt et al. 2010).
N.B. Antibody reactivity and working conditions may vary between species.
Tissue Culture Supernatant - liquid
Equine peripheral blood lymphocytes.
Approx. Protein Concentrations
Reagents In The Kit
Preparing The Antibody
Spleen cells from immunized LOU Rats were fused with cells of the Y3 Ag1.2.3. myeloma cell line.
Store at +4oC or at -20oC if preferred.
This product should be stored undiluted.
Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Immunohistology - Frozen
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recomended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Use 50ul of the suggested working dilution to label 7.5 x 105 cells in 100ul.
1. Tavernor, A.S. et al. (1994) Expression cloning of an equine T-lymphocyte glycoprotein CD2 cDNA. Structure-based analysis of conserved sequence elements.Eur J Biochem. 219 (3): 969-76.
2. Pearson, W. et al. (2007) Low-dose ginseng (Panax quinquefolium) modulates the course and magnitude of the antibody response to vaccination against equid herpesvirus I in horses. Can J Vet Res. 71: 213-7.