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Mouse anti Guinea pig CD45 antibody, clone IH-1 (MCA1130) used for the evaluation of CD45 expression on cultured bone marrow derived mesenchymal stem cells by flow cytometry.
Validation of MSC properties. a BM-MSCs were harvested and cultured from guinea pigs’ bone marrow from healthy (sham-exposed) or CS-exposed individuals. b Cell characterization was determined by plastic adhesion, colony forming capacity, and morphology. c Purity was checked by flow cytometric analysis of CD45 expression in BM-MSCs and compared with whole blood. Fluorescence intensity of the no-antibody (left panel), the secondary antibody control (middle panel) and fluorescence intensity in the presence of the CD45 specific antibody (right panel) was measured. The lower panel shows gene expression analysis of positive (CD105, CD90, CD73) and negative (CD11b, CD31, CD34) MSC markers and β-Actin. M: molecular weight; LS: lung tissue lysate. d Adipogenic and oesteogenic differentiation. Cells were incubated for 3 weeks in either induction or normal growth medium before von-Kossa and Alizarin red staining for calcium deposits or oil red to identify lipidic vacuoles
From: Tura-Ceide O, Lobo B, Paul T, Puig-Pey R, Coll-Bonfill N, García-Lucio J, Smolders V, Blanco I, Barberà JA, Peinado VI.
Cigarette smoke challenges bone marrow mesenchymal stem cell capacities in guinea pig.
Respir Res. 2017 Mar 23;18(1):50.