CD4 antibody | RPA-T4

Mouse anti Human CD4:Alexa Fluor® 647

Product Type
Monoclonal Antibody
Clone
RPA-T4
Isotype
IgG1
Specificity
CD4

Product Code Applications Pack Size List Price Your Price Qty
MCA1267A647
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
F 100 Tests/1ml loader
List Price Your Price
loader
MCA1267A647T
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
F 25 Tests/0.25ml loader
List Price Your Price
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Search for Batch Specific Datasheets

Mouse anti human CD4 antibody, clone RPA-T4 recognizes human CD4, a ~55 kDa cell surface glycoprotein, primarily expressed on a subpopulation of T lymphocytes, on peripheral blood monocytes and on tissue macrophages. Epitope mapping shows that antibodies, produced by clone RPA-T4, recognize an epitope within domain 1 of the extracellular region of the CD4 molecule.

Mouse anti human CD4 antibody, clone RPA-T4 blocks gp120-CD4 interaction and inhibits syncytium formation (Piatier-Tonneau et al, 1997). The use of Mouse anti Human CD4:Low Endotoxin (MCA1267EL) is recommended for functional assays.

Target Species
Human
Product Form
Purified IgG conjugated to Alexa Fluor® 647 - liquid
Preparation
MCA1267A647: Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
MCA1267A647T: Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09%Sodium Azide
1%Bovine Serum Albumin
Immunogen
Human PHA blasts
Approx. Protein Concentrations
IgG concentration 0.05 mg/ml
Fusion Partners
Spleen cells from immunized BALB/c mice were fused with cells of the mouse NSI myeloma cell line
Max Ex/Em
Fluorophore Excitation Max (nm) Emission Max (nm)
Alexa Fluor®647 650 665
Regulatory
For research purposes only
Guarantee
12 months from date of despatch
Acknowledgements
This product is provided under an intellectual property licence from Life Technologies Corporation. The transfer of this product is contingent on the buyer using the purchase product solely in research, excluding contract research or any fee for service research, and the buyer must not sell or otherwise transfer this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; (c) manufacturing or quality assurance or quality control, or (d) resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad CA 92008 USA or outlicensing@thermofisher.com

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry Neat 1/5 Pack Size: 100 Tests/1ml
1/10 Pack Size: 25 Tests/0.25ml
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 10ul of the suggested working dilution to label 106 cells or 100ul whole blood

Description Product Code Applications Pack Size List Price Your Price Quantity
Mouse IgG1 Negative Control:Alexa Fluor® 647 MCA928A647 F 100 Tests/1ml loader
List Price Your Price
loader
Description Mouse IgG1 Negative Control:Alexa Fluor® 647

Description Product Code Applications Pack Size List Price Your Price Quantity
Human Seroblock BUF070A F 50 Test
List Price Your Price
Description Human Seroblock
Human Seroblock BUF070B F 200 Test
List Price Your Price
Description Human Seroblock

References for CD4 antibody

  1. Zarkesh-Esfahani, H. et al. (2001) High-dose leptin activates human leukocytes via receptor expression on monocytes.
    J Immunol. 167 (8): 4593-9.
  2. Voehringer, D. et al. (2002) Lack of proliferative capacity of human effector and memory T cells expressing killer cell lectinlike receptor G1 (KLRG1).
    Blood. 100 (10): 3698-702.
  3. Piatier-Tonneau, D. (1997) CD4 workshop panel report.
    In: Leucocyte Typing VI: White Cell Differentiation Antigens: Proceedings of the Sixth International Workshop and Conference Held in Kobe, Japan, 10-14 November 1996. Garland Pub., 1998.
  4. Pentón-Rol, G. et al. (2011) C-Phycocyanin ameliorates experimental autoimmune encephalomyelitis and induces regulatory T cells.
    Int Immunopharmacol. 11 (1): 29-38.
  5. Wright, G.J. et al. (2001) The unusual distribution of the neuronal/lymphoid cell surface CD200 (OX2) glycoprotein is conserved in humans.
    Immunology. 102 (2): 173-9.
  6. Zhang, Y. et al. (2013) Accelerated in vivo. proliferation of memory phenotype CD4+ T-cells in human HIV-1 infection irrespective of viral chemokine co-receptor tropism.
    PLoS Pathog. 9 (4): e1003310.
  7. Bughani, U. et al. (2017) T cell activation and differentiation is modulated by a CD6 domain 1 antibody Itolizumab.
    PLoS One. 12 (7): e0180088.

Flow Cytometry

RRID
AB_324864
UniProt
P01730
Entrez Gene
CD4
GO Terms
GO:0001948 glycoprotein binding
GO:0007155 cell adhesion
GO:0016021 integral to membrane
GO:0004888 transmembrane receptor activity
GO:0007169 transmembrane receptor protein tyrosine kinase signaling pathway
GO:0005201 extracellular matrix structural constituent
GO:0005769 early endosome
GO:0005789 endoplasmic reticulum membrane
GO:0006948 induction by virus of host cell-cell fusion
GO:0006955 immune response
GO:0008270 zinc ion binding
GO:0015026 coreceptor activity
GO:0019059 initiation of viral infection
GO:0019901 protein kinase binding
GO:0030260 entry into host cell
GO:0031295 T cell costimulation
GO:0032507 maintenance of protein location in cell
GO:0042101 T cell receptor complex
GO:0042289 MHC class II protein binding
GO:0042803 protein homodimerization activity
GO:0045058 T cell selection
GO:0045086 positive regulation of interleukin-2 biosynthetic process
GO:0045234 protein palmitoleylation
GO:0045860 positive regulation of protein kinase activity
GO:0050690 regulation of defense response to virus by virus
GO:0050852 T cell receptor signaling pathway
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Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"

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