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CD45RA antibody | F8-11-13

Mouse anti Human CD45RA:StarBright Violet 710

Product Type
Monoclonal Antibody

Product Code Applications Pack Size List Price Your Price Qty
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
F 100 Tests/0.5ml loader
List Price Your Price

Mouse anti Human CD45RA antibody, clone F8-11-13 recognizes the human CD45RA cell surface antigen.

CD45 is a complex molecule existing in a number of isoforms. Antibodies recognizing a common epitope on all of these isoforms are termed CD45 whilst those recognizing only individual isoforms are termed CD45RA or CD45RO etc.

Mouse anti Human CD45RA antibody, clone F8-11-13 reacts with the high molecular weight form of the leucocyte common antigen (LCA) expressed by B lymphocytes and a subset of T lymphocytes.

Mouse anti Human CD45RA antibody, clone F8-11-13 is routinely tested in flow cytometry on human peripheral blood lymphocytes.

Target Species
Species Cross-Reactivity
Target SpeciesCross Reactivity
Rhesus Monkey
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG conjugated to StarBright Violet 710 - liquid
Purified IgG prepared by affinity chromatography on protein A from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
1% Bovine Serum Albumin
0.1% Pluronic F68
0.1% PEG 3350
0.05% Tween 20
Human T lymphocytes
Fusion Partners
Spleen cells from immunized BALB/c mouse were fused with cells of the NS-1 mouse myeloma cell line
Max Ex/Em
Fluorophore Excitation Max (nm) Emission Max (nm)
StarBright Violet 710 402 713
For research purposes only
12 months from date of despatch
This product is covered by U.S. Patent No. 10,150,841 and related U.S. and foreign counterparts

Store at +4°C. DO NOT FREEZE.
This product should be stored undiluted.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry Neat
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 5μl of the suggested working dilution to label 106 cells in 100μl. Best practices suggest a 5 minutes centrifugation at 6,000g prior to sample application.

How to Use the Spectraviewer

Watch the Tool Tutorial Video ▸
  • Start by selecting the application you are interested in, with the option to select an instrument from the drop down menu or create a customized instrument
  • Select the fluorophores or fluorescent proteins you want to include in your panel to check compatibility
  • Select the lasers and filters you wish to include
  • Select combined or multi-laser view to visualize the spectra

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References for CD45RA antibody

  1. Dalchau, R. & Fabre, J.W. (1981) Identification with a monoclonal antibody of a predominantly B lymphocyte-specific determinant of the human leukocyte common antigen. Evidence for structural and possible functional diversity of the human leukocyte common molecule.
    J Exp Med. 153 (4): 753-65.
  2. O'Grady, J.T. et al. (1994) CD40 expression in Hodgkin's disease.
    Am J Pathol. 144 (1): 21-6.
  3. Franitza, S. et al. (2002) TGF-beta1 enhances SDF-1alpha-induced chemotaxis and homing of naive T cells by up-regulating CXCR4 expression and downstream cytoskeletal effector molecules.
    Eur J Immunol. 32 (1): 193-202.
  4. Leigh, J.E. et al. (2006) Characterization of the immune status of CD8+ T cells in oral lesions of human immunodeficiency virus-infected persons with oropharyngeal Candidiasis.
    Clin Vaccine Immunol. 13 (6): 678-83.
  5. Hunt, L. et al. (2016) T cell subsets: an immunological biomarker to predict progression to clinical arthritis in ACPA-positive individuals.
    Ann Rheum Dis. 75 (10): 1884-9.
  6. Ibana, J.A. et al. (2012) The major CD8 T cell effector memory subset in the normal and Chlamydia trachomatis-infected human endocervix is low in perforin.
    BMC Immunol. 13: 66.
  7. Ponchel, F. et al. (2014) An immunological biomarker to predict MTX response in early RA.
    Ann Rheum Dis. 73 (11): 2047-53.

Immunohistology - Frozen

Entrez Gene
GO Terms
GO:0001915 negative regulation of T cell mediated cytotoxicity
GO:0001960 negative regulation of cytokine-mediated signaling pathway
GO:0002378 immunoglobulin biosynthetic process
GO:0005001 transmembrane receptor protein tyrosine phosphatase activity
GO:0005887 integral to plasma membrane
GO:0005925 focal adhesion
GO:0006469 negative regulation of protein kinase activity
GO:0006470 protein dephosphorylation
GO:0007411 axon guidance
GO:0019901 protein kinase binding
GO:0030890 positive regulation of B cell proliferation
GO:0033261 regulation of S phase
GO:0042100 B cell proliferation
GO:0045121 membrane raft
GO:0050852 T cell receptor signaling pathway
GO:0050853 B cell receptor signaling pathway
GO:0050857 positive regulation of antigen receptor-mediated signaling pathway
GO:0051209 release of sequestered calcium ion into cytosol
GO:0051607 defense response to virus



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