Overview

Bio-Rad has three fluorophores excitable by the red (640 nm) laser allowing an extra two colors to be added to any multicolor panel. Table 1 below shows the properties of these fluorophores.

Table 1: Fluorophores excited by the red (640 nm) laser.

Fluorophore

Ex Max (nm)

Em Max (nm)

Brightness

ZE5 Filter

Similar

APC

650

661

4

670/30

A647, Cy5, DyLight650

A647

650

665

4

670/30

A647, Cy5 DyLight650

A700

702

723

2

720/60

 


APC (ex 650/em 661)

Allophycocyanin (APC) is a fluorescent protein found in blue-green algae. It is a bright fluorophore with similar spectral characteristics to A647, therefore they can’t be used in the same panel.
 


 
APC (ex 650/em 661)

A647 (ex 650, em 665)

Alexa Fluor 647 is a bright photostable fluorophore suitable for flow cytometry and fluorescence microscopy. Due to similar excitation and emission characteristics to APC, they cannot be used together.
 


 
A647 (ex 650, em 665)

A700 (ex 700, em 702)

Alexa Fluor 700 is a relatively dim, far red fluorophore that gives extra multicolor panel options on the 640 nm laser.
 


 
A700 (ex 700, em 702)
Fig. 1. Alexa Fluor 700 staining

Fig. 1. Alexa Fluor 700 staining. Human peripheral blood stained with CD4 (MCA1267A700) and CD3 (MCA463F) after incubation with 10% human serum, to detect helper T cells. Data acquired on the ZE5 Cell Analyzer.


Fluorophores by Laser

Bio-Rad stocks a selection of different fluorophores that are usable with a range of lasers. Details of which fluorophores are best suited to which lasers can be found on our Fluorophores for Flow Cytometry page.

For more information about fluorophores and immunophenotyping, refer to our flow cytometry resources below.