Fluorophores for the UltraViolet (355 nm) Laser

Bio-Rad has new StarBright Dyes that are excitable by the ultraviolet (355 nm) laser and suitable for both conventional flow cytometry and spectral flow cytometry. Table 1 shows the excitation and emission maxima, the relative brightness, and ZE5 Cell Analyzer filter which allows detection. As other instruments may have different filters, please check compatibility on your instrument before you start your experiment.

Table 1. 355 nm excitable dyes for flow cytometry.

Fluorophore

Ex Max

Em Max

Brightness

ZE5 Cell Analyzer Filter

Similar

StarBright UltraViolet 400

335

395

3

387/11

BUV395

StarBright UltraViolet 445

347

440

3

447/60 or 460/22

Alexa Fluor350, DyL350

StarBright UltraViolet 510

340

513

3

509/25 or 525/50

BUV496

StarBright UltraViolet 575

340

569

4

577/15

BUV563

StarBright UltraViolet 605

340

609

4

615/24

BUV615

StarBright UltraViolet 665

340

669

4

670/30

BUV665

StarBright UltraViolet 740

344

743

4

747/33

BUV737

StarBright UltraViolet 795

340

792

2

780LP

BUV805


StarBright UltraViolet 400 Dye (ex 335/em 395)


StarBright UltraViolet 400 Dye is part of the StarBright Dye range, a new range of fluorescent nanoparticles specifically designed for flow cytometry to be bright with narrow excitation and emission profiles. Excitable by the ultraviolet, 355 nm laser and emitting at 395 nm, this bright fluorophore is the ideal dye to replace BUV395 giving you more choice and flexibility when multicolor panel building.
 

StarBright UltraViolet 400 Dye (ex 335/em 395)


StarBright UltraViolet 445 Dye (ex 347/em 440)


StarBright UltraViolet 445 Dye is part of the StarBright Dye range, a new range of fluorescent nanoparticles specifically designed for flow cytometry to be bright with narrow excitation and emission profiles. Excitable by the ultraviolet, 355 nm laser and emitting at 440 nm, this bright fluorophore is unique in that it is up to 20x brighter than AF350 and there are no polymer dyes that emit at a similar wavelength.
 

StarBright UltraViolet 445 Dye (ex 347/em 440)


StarBright UltraViolet 510 Dye (ex 340/em 513)


StarBright UltraViolet 510 Dye is part of the StarBright Dye range, a new range of fluorescent nanoparticles specifically designed for flow cytometry to be bright with narrow excitation and emission profiles. Excitable by the ultraviolet, 355 nm laser and emitting at 513 nm, this fluorophore is brighter than BUV496 and is not a traditional tandem dye giving you more choice and flexibility when multicolor panel building.

StarBright UltraViolet 510 Dye (ex 340/em 513)


StarBright UltraViolet 575 Dye (ex 340/em 569)


StarBright UltraViolet 575 Dye is part of the StarBright Dye range, a new range of fluorescent nanoparticles specifically designed for flow cytometry to be bright with narrow excitation and emission profiles. Excitable by the ultraviolet, 355 nm laser and emitting at 569 nm, this fluorophore is brighter than BUV563 and is not a traditional tandem dye giving you more choice and flexibility when multicolor panel building.

StarBright UltraViolet 575 Dye (ex 340/em 569)


StarBright UltraViolet 605 Dye (ex 340/em 609)


StarBright UltraViolet 605 Dye is part of the StarBright Dye range, a new range of fluorescent nanoparticles specifically designed for flow cytometry to be bright with narrow excitation and emission profiles. Excitable by the ultraviolet, 355 nm laser and emitting at 609 nm, this bright fluorophore with improved spectral properties is the ideal dye to replace BUV615 giving you more choice and flexibility when multicolor panel building.

StarBright UltraViolet 605 Dye (ex 340/em 609)


StarBright UltraViolet 665 Dye (ex 340/em 669)


StarBright UltraViolet 665 Dye is part of the StarBright Dye range, a new range of fluorescent nanoparticles specifically designed for flow cytometry to be bright with narrow excitation and emission profiles. Excitable by the ultraviolet, 355 nm laser and emitting at 669 nm, this bright fluorophore is the ideal dye to replace BUV661, particularly in panels containing A647, APC, and A700 as it has less excitation by the 640 nm laser giving you more choice and flexibility when multicolor panel building.

StarBright UltraViolet 665 Dye (ex 340/em 669)


StarBright UltraViolet 740 Dye (ex 344/em 743) Coming Soon
StarBright UltraViolet 740 Dye (ex 344/em 743)

StarBright UltraViolet 795 Dye (ex 340/em 792)


StarBright UltraViolet 795 Dye is part of the StarBright Dye range, a new range of fluorescent nanoparticles specifically designed for flow cytometry to be bright with narrow excitation and emission profiles. Excitable by the ultraviolet, 355 nm laser and emitting at 792 nm, this fluorophore is twice as bright as BUV805 giving you more choice and flexibility when multicolor panel building.

StarBright UltraViolet 795 Dye (ex 340/em 792)


StarBright UltraViolet Antibodies

    DescriptionSpecificityTargetFormatHostIsotypeClone Applications Citations Product Type Code Validation Types

    Fluorophores by Laser

    Bio-Rad stocks a selection of different fluorophores that are suitable for use with a range of lasers. Details of which fluorophores are best suited to which lasers can be found on our Fluorophores for Flow Cytometry page.

    For more information about fluorophores and immunophenotyping, refer to our flow cytometry resources below.