IgA Antibody

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  • Sheep anti Bovine IgA
  • Sheep anti Bovine IgA:Biotin
  • Sheep anti Bovine IgA:FITC
  • Sheep anti Bovine IgA:HRP
  • Sheep anti Bovine IgA:Alk. Phos.
(Rated 0.0 out of 5 based on 0 customer reviews)
  • Product Type
    Polyclonal Antibody
  • Isotype
    Polyclonal IgG
5 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    AAI49ABC, E, IC, WBdatasheet pdfdatasheet pdf0.5 mg
    AAI49AB
    AAI49C, E *, IC, WBdatasheet pdfdatasheet pdf1 mg
    AAI49
    AAI49BC, E, IC, WBdatasheet pdfdatasheet pdf1 mg
    AAI49B
    AAI49FIFdatasheet pdfdatasheet pdf1 mg
    AAI49F
    AAI49PC, E, IC, WBdatasheet pdfdatasheet pdf1 mg
    AAI49P
    Summary
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    More Images
    References
    Reviews
    -
    • Sheep anti Bovine IgA antibody recognizes bovine IgA and shows no cross reactivity with other bovine immunoglobulin classes in immunoelectrophoresis.This antibody may recognize IgA from other species, though this has not been verified.

      IgA is the predominant surface secreted immunoglobulin from the intestinal, urogenital and respiratory tracts. IgA functions in a protective capacity, primarily, by preventing viruses and bacteria from adhearing to epithelial tissues. High concentrations of IgA are found in saliva, colostrum, milk, tear duct fluid, urine and nasal fluid.

      In addition to this polyclonal antibody, We have a monoclonal Mouse anti Bovine IgA , clone IL-A71 antibody (MCA2438GA ) available which is specific for bovine IgA, also available in a peroxidase conjugated format.
    • Intended Use
    • Target Species
      Bovine
    • Product Form
      Purified IgG - liquid
      Purified IgG conjugated to Biotin - liquid
      Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid
      Purified IgG conjugated to Horseradish Peroxidase (HRP) - liquid
      Purified IgG conjugated to Alkaline Phosphatase - liquid
    • Reconstitution
    • Preparation
    • Antiserum Preparation
      Antisera to bovine IgA were raised by repeated immunisation of sheep with highly purified antigen. Purified IgG prepared by affinity chromatography.
    • Preservative Stabilisers
      0.09% Sodium Azide (NaN3)
      0.09% Sodium Azide (NaN3)
      0.2% Bovine Serum Albumin
      0.09% Sodium Azide (NaN3)
      0.2% Bovine Serum Albumin
      0.1% Proclin™ 300
      0.2% Bovine Serum Albumin
      0.09% Sodium Azide (NaN3)
      0.2% Bovine Serum Albumin
    • Immunogen
      Purified bovine IgA
    • Purity
    • Approx. Protein Concentrations
      IgG concentration 1.0 mg/ml
      IgG concentration 1.0 mg/ml
      IgG concentration 1.0 mg/ml
      IgG concentration 1.0 mg/ml
      IgG concentration 0.5 mg/ml
    • Reagents In The Kit
    • Preparing The Antibody
    • Test Principle
    • Buffer Solution
      Phosphate buffered saline
      Phosphate buffered saline
      Phosphate buffered saline
      Phosphate buffered saline
      50mM HEPES, 0.1M NaCl, 1mM MgCl2, 0.1mM ZnCl2
    • Storage
      Store at +4oC. DO NOT FREEZE.
      This product should be stored undiluted.
      Store at +4oC. DO NOT FREEZE.
      This product should be stored undiluted.
      Store at +4oC. DO NOT FREEZE.
      This product should be stored undiluted. This product is photosensitive and should be protected from light.
      Store at +4oC. DO NOT FREEZE.
      This product should be stored undiluted. Should this product contain a precipitate we recommend microcentrifugation before use.
      Store at +4oC. DO NOT FREEZE.
      This product should be stored undiluted.
    • Shelf Life
      12 months from date of despatch
      12 months from date of despatch
      12 months from date of despatch
      12 months from date of despatch
      12 months from date of despatch
    • Acknowledgements
      Proclin™ 300 is a trademark of The Dow Chemical Company (“Dow”) or an affiliated company of Dow.
    • Regulatory
      For research purposes only
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution
      Immunocytochemistry1/2001/2000
      Immunohistology - Frozen1/2001/2000
      Western Blotting1/10001/30,000
      ELISA(1)1/10001/30,000
      (1)
      For coating plates it is recommended that a dilution of 1/100 - 1/500 be used
    • Application NameYesNoMin DilutionMax Dilution
      ELISA1/10,0001/200,000
      Immunocytochemistry1/1001/500
      Immunohistology - Frozen1/2501/2500
      Western Blotting1/10,0001/200,000
    • Application NameYesNoMin DilutionMax Dilution
      Immunofluorescence1/501/100
    • Application NameYesNoMin DilutionMax Dilution
      ELISA1/10,0001/100,000
      Immunocytochemistry1/2001/2000
      Immunohistology - Frozen1/2001/2000
      Western Blotting1/20001/20,000
    • Application NameYesNoMin DilutionMax Dilution
      ELISA1/10001/10,000
      Immunocytochemistry1/1001/1000
      Immunohistology - Frozen1/1001/1000
      Western Blotting1/10001/10,000

    • Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
    • Technical Advice
    • Technical Advice
    • Technical Advice
    • Technical Advice
    • Technical Advice
    • Recommended Protocol
    • Recommended Protocol
    • Recommended Protocol
    • Recommended Protocol
    • Recommended Protocol
    • ELISA
    • ELISA
    • ELISA
    • ELISA
    • ELISA
    • Immunohistology
    • Immunohistology
    • Immunohistology
    • Immunohistology
    • Immunohistology
    • Histology Positive Control Tissue
    • Histology Positive Control Tissue
    • Histology Positive Control Tissue
    • Histology Positive Control Tissue
    • Histology Positive Control Tissue
    • Immunofluorescence
    • Immunofluorescence
    • Immunofluorescence
    • Immunofluorescence
    • Immunofluorescence
    • Western Blotting
    • Western Blotting
    • Western Blotting
    • Western Blotting
    • Western Blotting
    • Instructions For Use
    • Instructions For Use
    • Instructions For Use
    • Instructions For Use
    • Instructions For Use

    Additional IgA Antibody Formats

    Formats Applications Sizes available
    IgA Antibody : Alk. Phos. C, E, IC, WB 0.5 mg
    IgA Antibody : FITC IF 1 mg
    IgA Antibody : HRP C, E, IC, WB 1 mg
    IgA Antibody : Purified C, E *, IC, WB 1 mg
    IgA Antibody : Biotin C, E, IC, WB 1 mg
    • Copyright © 2016 Bio-Rad Antibodies (formerly AbD Serotec)

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      Recommended Negative Isotype Control

        Useful Reagents

          Recommended Positive Controls

            Histology Controls

              Further Reading

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