Bovine Dendritic Cell Growth Kit

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Bovine Dendritic Cell Growth Kit gallery image 1

Bovine CD14 enriched cells visualised by light microscopy. A - 2 day cultured unstimulated cells; B - 2 day cultured cells in bovine dendritic cell growth kit (PBP014KZZ) at 1:20 dilution

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  • Bovine Dendritic Cell Growth Kit
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  • Product Type
    Kits
1 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    PBP014KZZFNdatasheet pdfdatasheet pdf1 ml
    PBP014KZZ
    Summary
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    More Images
    References
    Reviews
    -
    • PBP014KZZ is contains a cocktail of biologically active interleukin-4 (IL-4) and granulocyte/macrophage-colony stimulating factor (GM-CSF) that have been premixed at optimal concentrations to induce dendritic cell development from peripheral blood-derived bovine (cattle) monocytes.
    • Intended Use
    • Target Species
      Bovine
    • Product Form
      Mixed recombinant bovine Interleukin-4 and bovine GM-CSF – supplied as a liquid
    • Reconstitution
    • Preparation
      Recombinant cytokines expressed in mammalian Chinese Hamster Ovary (CHO) cells using the pEE14® vector grown in antibiotic free media and USDA-approved dialysed FCS which has been screened for BVDV and virus growth by PCR.
    • Preservative Stabilisers
      None present
    • Purity
    • Approx. Protein Concentrations
    • Reagents In The Kit
    • Preparing The Antibody
    • Endotoxin Level
      <0.5EU/mL
    • Test Principle
    • Buffer Solution
    • Storage
      Store at -20oC only.
      Storage in frost-free freezers is not recommended.
      This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature this recombinant protein. Should this product contain a precipitate we recommend microcentrifugation before use.
    • Shelf Life
      6 months from date of despatch.
    • Acknowledgements
      This reagent was produced as part of the BBSRC/SEERAD Immunological Toolbox. The kit development was also supported by the European Community’s Seventh Framework Programme (FP7, 2007-2013), Research Infrastructures action, under the grant agreement No. FP7-228394 (NADIR)
    • Regulatory
      For research purposes only
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution
      Functional Assays1:20

    • Where this reagent has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the reagent for use in their own system using appropriate negative/positive controls.
    • Technical Advice
    • Recommended Protocol
      1. Prepare peripheral blood mononuclear cells (PBMC) from heparinised blood by density gradient centrifugation.

      2. Purify CD14+ve cells by labelling PBMC with CD14 mAb and utilise magnetic bead or flow cytometric separation techniques.

      3. Resuspend the isolated CD14+ve cells at a concentration of 1x106 cells/ml in tissue culture medium (TCM = RPMI or equivalent + 10% foetal calf serum) containing a final dilution of 1:20 of PBP014KZZ .

      4. Add 3ml of cell suspension to each well of a 6 well tissue culture plate.

      5. Culture cells in a humidified atmosphere of 5% CO2 in air, at approximately 37oC.

      6. Culture cells for 3 days. The cells may then be harvested and used for other procedures including immunophenotyping (as required).

      7. If a longer culture period is required the cells must be ‘fed’ with new TCM containing cytokines on day 3:
      Carefully remove 1ml spent medium from each well, care is required to avoid disturbing the cells.
      Add 1.5ml fresh, pre-warmed TCM containing cytokines at 1:20 to each well and re-culture the DC for required culture period (typically up to 7 days).

      8. At the end of the culture period adherent and non-adherent cells can be pooled for use in immunoassays and phenotyped (as required). Adherent cells may require a dissociation step to remove them from the plate.
    • ELISA
    • Immunohistology
    • Histology Positive Control Tissue
    • Immunofluorescence
    • Western Blotting
    • Instructions For Use

    Additional Bovine Dendritic Cell Growth Kit Formats

    Formats Applications Sizes available
    Bovine Dendritic Cell Growth Kit : Kit FN 1 ml
    • Copyright © 2016 Bio-Rad

    Recommended Secondary Antibody

      Recommended Negative Isotype Control

        Useful Reagents

          DescriptionProduct CodePack SizeApplicationsList PriceQuantity
          Mouse anti Human CD14:Alexa Fluor® 647MCA1568A647100 Tests/1mlF
          MCA1568A647
          Mouse anti Human CD14:Alexa Fluor® 700MCA1568A700100 Tests/1mlF
          MCA1568A700
          Mouse anti Human CD14:BiotinMCA1568B0.1 mgF
          MCA1568B
          Mouse anti Human CD14:FITCMCA1568F0.1 mgF
          MCA1568F
          Mouse anti Bovine CD14:FITCMCA2678F0.1 mgF
          MCA2678F
          Mouse anti Bovine MHC Class II DQ:FITCMCA5655F0.1 mgF
          MCA5655F
          Mouse anti Human CD14:Low EndotoxinMCA1568EL0.5 mgE, F, FN, WB
          MCA1568EL
          Mouse anti Human CD14:Pacific Blue®MCA1568PB100 Tests/1mlF
          MCA1568PB
          Mouse anti Bovine MHC Class II DQMCA56550.25 mgF, IF
          MCA5655
          Mouse anti Bovine MHC Class II DRMCA56560.25 mgF
          MCA5656
          Mouse anti Bovine CD1w2MCA831G0.25 mgC, F, IP
          MCA831G
          Mouse anti Human CD14:RPE-Alexa Fluor® 647MCA1568P647100 Tests/1mlF
          MCA1568P647
          Mouse anti Human CD14:RPEMCA1568PE100 TestsF
          MCA1568PE

          Recommended Positive Controls

            Histology Controls

              References

              Further Reading

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