Mouse IgG1 Negative Control antibody
Mouse IgG1 isotype control (MCA928) used to evaluate background staining on human mesenchymal stem cells by flow cytometry.
Image caption:
Phenotypic characterization of human adipose-derived MSCs. a The fibroblast-like morphology of adhesion cells was studied by phase-contrast microscopy. Scale 100 μm. b Multilineage differentiation of MSCs. Histochemical staining of calcium deposits (alizarin red) and neutral lipid accumulation (oil red O) after 14 days of osteogenesis and adipogenesis induction. Scale 100 μm. c Flow cytometry analysis of TLR3 expression: after 10 µg/mL poly(I:C) stimulation for 3 h – prMSCs (red line) and without stimulation – MSCs (constitutive expression, blue line), isotypic control—IC (green line). d, e Immunophenotypic characteristics of MSCs and prMSCs: negative expression of CD34, CD45, and HLA-DR markers (top panel); positive expression of CD73, CD90, and CD105 markers (bottom panel)
From: Tolstova T, Dotsenko E, Kozhin P, Novikova S, Zgoda V, Rusanov A, Luzgina N.
The effect of TLR3 priming conditions on MSC immunosuppressive properties.
Stem Cell Res Ther. 2023 Nov 29;14(1):344.
doi: 10.1186/s13287-023-03579-y.
This image is from an open access article distributed under terms of a Creative Commons Attribution License.
Filter by Application:
F FLA ResetMouse IgG1 Negative Control
- Product Type
- Negative/Isotype Control
- Isotype
- IgG1
- Specificity
- Mouse IgG1 Negative Control
Antibody Quality and Performance Guarantee
| Mouse IgG1 negative control is negative by flow cytometry on all human cells and cell lines tested. Further tests have also shown that this reagent is also suitable for use as a negative control with bovine ( This reagent recognizes a rat cell surface marker, and therefore cannot be used as a negative control in this species. |
- Target Species
- Negative Control
- Product Form
- Purified IgG - liquid
- Preparation
- Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
0.09% Sodium Azide 1% Bovine Serum Albumin - Approx. Protein Concentrations
- IgG concentration 0.1mg/ml
- Regulatory
- For research purposes only
- Guarantee
- 12 months from date of despatch
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Flow Cytometry |  |
* |
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. *It is recommended that the user titrates the antibody for use in their own system to a concentration equivalent to that of their test reagents.
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 106 cells or 100ul whole blood
References for Mouse IgG1 Negative Control antibody
-
Kupatt, C. et al. (2000) c7E3Fab reduces postischemic leukocyte-thrombocyte interaction mediated by fibrinogen. Implications for myocardial reperfusion injury.
Arterioscler Thromb Vasc Biol. 20 (10): 2226-32. -
Jacks, S. et al. (2007) Experimental infection of neonatal foals with Rhodococcus equi triggers adult-like gamma interferon induction.
Clin Vaccine Immunol.14:669-77 -
Pakandl, M. et al. (2008) Immune response to rabbit coccidiosis: a comparison between infections with Eimeria flavescens and E. intestinalis.
Folia Parasitol (Praha). 55:1-6. -
Dalli, J. et al. (2008) Annexin 1 mediates the rapid anti-inflammatory effects of neutrophil-derived microparticles.
Blood. 112 (6): 2512-9. -
Barratt-Due, A. et al. (2011) Ornithodoros moubata Complement Inhibitor Is an Equally Effective C5 Inhibitor in Pigs and Humans.
J Immunol. 187: 4913-9. -
Maślanka, T. et al. (2012) The presence of CD25 on bovine WC1+ gammadelta T cells is positively correlated with their production of IL-10 and TGF-beta, but not IFN-gamma.
Pol J Vet Sci. 15 (1): 11-20. -
Maiolini, A. et al. (2012) Toll-like receptors 4 and 9 are responsible for the maintenance of the inflammatory reaction in canine steroid-responsive meningitis-arteritis, a large animal model for neutrophilic meningitis.
J Neuroinflammation. 9: 226. -
Kapetanovic, R. et al. (2012) Pig bone marrow-derived macrophages resemble human macrophages in their response to bacterial lipopolysaccharide.
J Immunol. 188: 3382-94.
View The Latest Product References
-
Kamble, N.M. et al. (2016) Interaction of a live attenuated Salmonella Gallinarum vaccine candidate with chicken bone marrow-derived dendritic cells.
Avian Pathol. 45 (2): 235-43. -
Iwaszko-Simonik, A. et al. (2015) Expression of surface platelet receptors (CD62P and CD41/61) in horses with recurrent airway obstruction (RAO).
Vet Immunol Immunopathol. 164 (1-2): 87-92. -
Brace, P.T. et al. (2017) Mycobacterium tuberculosis subverts negative regulatory pathways in human macrophages to drive immunopathology.
PLoS Pathog. 13 (6): e1006367. -
Topoluk, N. et al. (2017) Amniotic Mesenchymal Stromal Cells Exhibit Preferential Osteogenic and Chondrogenic Differentiation and Enhanced Matrix Production Compared With Adipose Mesenchymal Stromal Cells.
Am J Sports Med. 45 (11): 2637-46. -
Arzi, B. et al. (2017) Therapeutic Efficacy of Fresh, Allogeneic Mesenchymal Stem Cells for Severe Refractory Feline Chronic Gingivostomatitis.
Stem Cells Transl Med. 6 (8): 1710-22. -
Taechangam, N. et al. (2021) Feline adipose-derived mesenchymal stem cells induce effector phenotype and enhance cytolytic function of CD8+ T cells.
Stem Cell Res Ther. 12 (1): 495. -
do Prado Duzanski, A.et al. (2022) Cell-mediated immunity and expression of MHC class I and class II molecules in dogs naturally infected by canine transmissible venereal tumor: Is there complete spontaneous regression outside the experimental CTVT?
Research in Veterinary Science. 145: 193-204. -
Tolstova, T. et al. (2023) The effect of TLR3 priming conditions on MSC immunosuppressive properties.
Stem Cell Res Ther. 14 (1): 344. -
Geng, Y. et al. (2018) Dietary vitamin D(3) supplementation protects laying hens against lipopolysaccharide-induced immunological stress.
Nutr Metab (Lond). 15: 58. -
Dan-Jumbo, S.O. et al. (2024) Derivation and long-term maintenance of porcine skeletal muscle progenitor cells.
Sci Rep. 14 (1): 9370. -
Maciag, S. et al. (2022) Effects of freezing storage on the stability of maternal cellular and humoral immune components in porcine colostrum.
Vet Immunol Immunopathol. 254: 110520. -
Forner, R. et al. (2021) Distribution difference of colostrum-derived B and T cells subsets in gilts and sows.
PLoS One. 16 (5): e0249366.
- RRID
- AB_322259
MCA928
0715
151536
160961
1808
If you cannot find the batch/lot you are looking for please contact our technical support team for assistance.
Request a different product with this specificity
Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"
View all Negative Control ProductsAlways be the first to know.
When we launch new products and resources to help you achieve more in the lab.
Yes, sign me up