Cl:A3-1 Antibody | 17867

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Cl:A3-1 Antibody | 17867 gallery image 1

Frozen mouse spleen stained with A: Rat anti Mouse F4/80 (MCA497) or B: Rat anti Mouse F4/80 (MCA497) preincubated with a 2-fold molar excess of Human anti idiotypic Cl:A3-1 (F4/80) (clone 17867). Detection was performed using Goat anti Rat IgG:HRP (STAR72)

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  • Human anti Idiotypic Cl:A3-1 (F4/80)
  • Human anti Idiotypic Cl:A3-1 (F4/80)
(Rated 0.0 out of 5 based on 0 customer reviews)
  • Product Type
    Monoclonal Antibody
  • Clone
    17867
  • Isotype
    HuCAL Fab monovalent
1 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    HCA154Cdatasheet pdfdatasheet pdf0.1 mg
    HCA154
    HCA154TCdatasheet pdfdatasheet pdf25 µg
    HCA154T
    Summary
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    More Images
    References
    Reviews
    -
    • Human anti Idiotypic CI:A3-1(F4/80), clone 17867, is anti idiotypic antibody specifically raised against the monoclonal rat anti mouse F4/80 antibody, clone CI:A3-1 (MCA497).

      Clone 17867 specifically binds to and blocks Cl:A3-1. This reagent was developed and designed as a control antibody for use in histology studies in conjunction with Cl:A3-1.

      Human anti Idiotypic CI:A3-1(F4/80), clone 17867 forms part of an expanding portfolio of anti-idiotypic reagents being developed and can be viewed here.
    • Intended Use
    • Product Form
      A monovalent human recombinant Fab selected from the HuCAL® phage display library, expressed in E. coli. The antibody is tagged with a DYKDDDDK tag and a double extended Strep-tag at the C-terminus of the antibody heavy chain. This antibody is supplied as a liquid.
      A monovalent human recombinant Fab selected from the HuCAL® phage display library, expressed in E. coli. The antibody is tagged with a DYKDDDDK tag and a double extended Strep-tag at the C-terminus of the antibody heavy chain. This antibody is supplied as a liquid.
    • Reconstitution
    • Preparation
    • Preservative Stabilisers
      0.09% Sodium Azide (NaN3)
      0.09% Sodium Azide (NaN3)
    • Immunogen
      Clone Cl:A3-1 (anti F4/80).
    • Purity
    • Approx. Protein Concentrations
      Total protein concentration 0.5 mg/ml
      Total protein concentration 0.5 mg/ml
    • Reagents In The Kit
    • Preparing The Antibody
    • Test Principle
    • Buffer Solution
      Phosphate buffered saline
      Phosphate buffered saline
    • Storage
      Store at +4oC or at -20oC if preferred.
      Storage in frost-free freezers is not recommended.
      This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
      Store at +4oC or at -20oC if preferred.
      Storage in frost-free freezers is not recommended.
      This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
    • Shelf Life
      12 months from date of despatch.
      12 months from date of despatch
    • Acknowledgements
      Sold under license of U.S. Patents 6,300,064, 6,696,248, 6,708,484, 6,753,136, European patent 0,859,841 and corresponding patents.
      This antibody was developed by Bio-Rad, a wholly owned subsidiary of Bio-Rad Laboratories Inc., Zeppelinstr. 4, 82178 Puchheim, Germany.
      Sold under license of U.S. Patents 6,300,064, 6,696,248, 6,708,484, 6,753,136, European patent 0,859,841 and corresponding patents.
      This antibody was developed by Bio-Rad, a wholly owned subsidiary of Bio-Rad Laboratories Inc., Zeppelinstr. 4, 82178 Puchheim, Germany.
    • Licensed Use
      For in vitro research purposes only, unless otherwise specified in writing by Bio-Rad.
    • Regulatory
      For research purposes only
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution
      Immunohistology - Frozen5ug/ml
    • Application NameYesNoMin DilutionMax Dilution
      Immunohistology - Frozen5ug/ml

    • Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only.
    • Technical Advice
      Recommended protocols and further information about HuCAL recombinant antibody technology can be found in the HuCAL Antibodies Technical Manual
    • Technical Advice
      Recommended protocols and further information about HuCAL recombinant antibody technology can be found in the HuCAL Antibodies Technical Manual
    • Recommended Protocol
    • Recommended Protocol
    • ELISA
    • ELISA
    • Immunohistology
    • Immunohistology
    • Histology Positive Control Tissue
    • Histology Positive Control Tissue
    • Immunofluorescence
    • Immunofluorescence
    • Western Blotting
    • Western Blotting
    • Instructions For Use
    • Instructions For Use

    Additional Cl:A3-1 Antibody Formats

    Formats Clone Applications Sizes available
    Cl:A3-1 Antibody : Purified 17867 C 25 µg | 0.1 mg
    • Copyright © 2016 Bio-Rad

    Recommended Secondary Antibody

      Recommended Negative Isotype Control

        Useful Reagents

          DescriptionProduct CodePack SizeApplicationsList PriceQuantity
          Rat anti Mouse F4/80MCA497GA0.1 mgC, EM, F, IF, IP, P *, R, RE, WB
          MCA497GA
          Rat anti Mouse F4/80MCA497G0.5 mgC, EM, F, IF, IP, P *, R, RE, WB
          MCA497G
          Rat anti Mouse F4/80MCA4972 mlC, EM, F, IF, IP, P *, R, RE
          MCA497
          DescriptionProduct CodePack SizeApplicationsList PriceQuantity
          Rat anti Mouse F4/80MCA497GA0.1 mgC, EM, F, IF, IP, P *, R, RE, WB
          MCA497GA
          Rat anti Mouse F4/80MCA497G0.5 mgC, EM, F, IF, IP, P *, R, RE, WB
          MCA497G
          Rat anti Mouse F4/80MCA4972 mlC, EM, F, IF, IP, P *, R, RE
          MCA497

          Recommended Positive Controls

            Histology Controls

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