IFN Gamma Antibody

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Published customer image:
Rabbit anti Chicken interferon-gamma antibody used for the evaluation of interferon-γ producing T-cells by flow cytometry.
Image caption:
(A) The frequency of IFN-γ+CD4+ or IFN-γ+CD8+ T cells in lungs. Chickens in groups of five were vaccinated with mock vaccine or SD/01/10-ca on day 0 and challenged intranasally with 106 EID50 of A/chicken/Hebei/YT/2010 on day 14 after vaccination. Lungs were harvested on day 7 after challenge. IFN-γ+CD4+ or IFN-γ+CD8+ T cells in lungs stimulated with SD/01/10-wt were measured. Percentages of IFN-γ+CD4+ or IFN-γ+CD8+ T cells within CD4+ or CD8+ T cells were analyzed. *P <0.05, by one-way analysis of variance of the difference between SD/01/10-ca and mock vaccine group on day 7 after challenge. (B) HI antibody responses to homologous and heterologous viruses after vaccination with SD/01/10-ca. Serum samples were collected on day 14 post immunization, and the reactivity of collected sera (n = 3) to SD/01/10-wt or five heterologous H9N2 influenza viruses was assessed by an HI assay. Abbreviations: BJ, Beijing; Ck, chicken; GD, Guangdong; HeB, Hebei; SD, Shandong.

From: Wei Y, Qi L, Gao H, Sun H, Pu J, Sun Y, Liu J.
Generation and protective efficacy of a cold-adapted attenuated avian H9N2 influenza vaccine.
Sci Rep. 2016 Jul 26;6:30382.

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  • Rabbit anti Chicken Interferon Gamma:Preservative Free
(Rated 0.0 out of 5 based on 0 customer reviews)
  • Product Type
    Polyclonal Antibody
  • Isotype
    Polyclonal IgG
1 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    AHP945ZE, FN, WBdatasheet pdfdatasheet pdf0.5 mg
    AHP945Z
    Summary
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    More Images
    References
    Reviews
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    • Rabbit anti Chicken interferon gamma antibody recognizes chicken Interferon gamma (chIFN-gamma).There are at least three distinct chicken interferon proteins, designated as chIFN-alpha, chIFN-beta and chIFN-gamma.

      chIFN-gamma was first identified, when supernatant from chicken spleen cells stimulated with concanavalin A (ConA ), displayed macrophage-activating factor (MAF) activity - the principle function of mammalian type II IFN-gamma (Vonbulow et al. 1984).Cloning of IFN-gamma cDNA from activated chicken T cells indicates that recombinant chicken IFN-gamma activates macrophages, strongly upregulating the expression of MHC class II and iNOS genes (Weining et al. 1996).

      Rabbit anti Chicken interferon gamma antibody an be used to neutralize chIFN-gamma activity and to discriminate the antiviral activity of chIFN-gamma from that of chIFN-alpha and chIFN-beta (Weining et al. 1996).
    • Intended Use
    • Target Species
      Chicken
    • Product Form
      Purified IgG - liquid
    • Reconstitution
    • Preparation
    • Antiserum Preparation
      Antisera to chicken Interferon Gamma were raised by repeated immunisations of rabbits with highly purified antigen. Purified IgG was prepared by affinity chromatography on protein G.
    • Preservative Stabilisers
      None present.
    • Immunogen
      Recombinant chicken interferon gamma
    • Purity
    • Approx. Protein Concentrations
      IgG concentration 0.5 mg/ml
    • Reagents In The Kit
    • Preparing The Antibody
    • Test Principle
    • Buffer Solution
      Phosphate buffered saline
    • Storage
      Store at -20oC only.
      Storage in frost-free freezers is not recommended.
      This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
    • Shelf Life
      18 months from date of despatch.
    • GO Terms
      immune response
      cytokine activity
      interferon-gamma receptor binding
      extracellular space
      response to virus
    • UniProt
    • Entrez Gene
    • Acknowledgements
    • Regulatory
      For research purposes only
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution
      ELISA
      Functional Assays10ug/ml50ug/ml
      Western Blotting

    • Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
    • Technical Advice
    • Recommended Protocol
    • ELISA
    • Immunohistology
    • Histology Positive Control Tissue
    • Immunofluorescence
    • Western Blotting
      AHP945Z detects a band of approximately 18 kDa.
    • Instructions For Use

    Additional IFN Gamma Antibody Formats

    Formats Applications Sizes available
    IFN Gamma Antibody : Preservative Free E, FN, WB 0.5 mg
    • Copyright © 2016 Bio-Rad

    Recommended Secondary Antibody

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      STAR35A

      Recommended Negative Isotype Control

        Useful Reagents

          DescriptionProduct CodePack SizeApplicationsList PriceQuantity
          TidyBlot™ Western Blot Detection Reagent:HRPSTAR209P0.5 mlWB*
          STAR209P

          Recommended Positive Controls

            Histology Controls

              References

              Further Reading

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