Mouse Seroblock FcR | FCR4G8

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Mouse Seroblock FcR

Product Type
Accessory Reagent
Clone
FCR4G8
Isotype
IgG2b
Product Code Applications Pack Size List Price Quantity
BUF041A
Datasheet
F
SDS
0.1 mg loader

BUF041B
Datasheet
F
SDS
0.5 mg loader

Product Details

Target Species
Mouse
Product Form
Purified IgG - liquid
Preparation
Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09%Sodium Azide (NaN3)
Approx. Protein Concentrations
IgG concentration 1.0mg/ml

Storage Information

Storage
Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Guarantee
12 months from date of despatch

More Information

Regulatory
For research purposes only

Applications of Mouse Seroblock FcR

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Instructions For Use
In order to reduce Fc-receptor mediated binding of test antibodies the following procedure is recommended:-

1) Incubate the cell suspension (1 x 106 cells in 100ul) with 1ug of BUF041A (1ul of undiluted reagent, or 10ul of a 1/10 dilution) for 5-10 minutes.

2) Add test antibody according to manufacturers instructions – Do not wash BUF041A off the cells. BUF041A is suitable for use in conjunction with test antibodies from any manufacturer or with in-house antibodies.*

3) Proceed with staining as usual.

* Care is needed in the design of experiments utilizing unconjugated anti-mouse antibodies, to ensure that the secondary antibody being used does not cross-react with SeroBlock FcR. Bio-Rad supply a range of isotype specific anti-mouse immunoglobulin antibodies that may be useful for this purpose.

Product Specific References

References for Mouse Seroblock FcR

  1. Ivanovska, N.D et al . (2008) Properdin Deficiency in Murine Models of Nonseptic Shock.
    The Journal of Immunology. 180: 6962-6969.
  2. Chen, H-F et al . (2009) A reduced oxygen tension (5%) is not beneficial for maintaining human embryonic stem cells in the undifferentiated state with short splitting intervals.
    Human Reproduction. 24: 71-80.
  3. Birjandi, S.Z. et al. (2011) Alterations in marginal zone macrophages and marginal zone B cells in old mice.
    J Immunol. 186: 3441-51.
  4. Cousins, F.L. et al. (2016) Evidence for a dynamic role for mononuclear phagocytes during endometrial repair and remodelling.
    Sci Rep. 6: 36748.
  5. Bi, C.L. et al. (2020) LncRNA NEAT1 promotes malignant phenotypes and TMZ resistance in glioblastoma stem cells by regulating let-7g-5p/MAP3K1 axis.
    Biosci Rep. 40 (10) [Epub ahead of print].

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