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Mouse Seroblock FcR | FCR4G8

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Mouse Seroblock FcR, clone FCR4G8 thumbnail image 1
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Hamster anti Mouse TCR Gamma/Delta:RPE antibody (MCA1366PE) staining mouse spleen cells in the absence (blue line) and presence (red histogram) of SeroBlock FcR (BUF041B).

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Mouse Seroblock FcR

Product Type
Accessory Reagent
Clone
FCR4G8
Isotype
IgG2b
Specificity
Mouse Seroblock FcR

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Technical Suppport
Product Code Applications Pack Size List Price Your Price Qty
BUF041A
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
F 0.1 mg loader
List Price Your Price
loader
BUF041B
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
F 0.5 mg loader
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loader
Search for Batch Specific Datasheets

Mouse Seroblock FcR is a rat antibody that recognizes mouse CD16 and CD32, cell surface proteins also known as FcRγIII and FcRγII, respectively. The function of these proteins is to bind IgG molecules via their Fc regions as part of the adaptive immune response. CD16 and/or CD32 are expressed by a wide variety of cells, including monocytes, macrophages, B lymphocytes, granulocytes, NK cells, dendritic cells and some activated T lymphocytes. The expression of CD16/CD32 antigens can lead to non-specific binding of test monoclonal antibodies in staining procedures, resulting in “high background” staining on a wide range of cells. This non-specific binding may be blocked by pre-incubation of target cells with BUF041A, resulting in clearer staining. For direct analysis of CD16/32 expression this antibody is also available conjugated directly to FITC (MCA2305F ), AlexaFluor488 (MCA2305A488), AlexaFluor647 (MCA2305A647 ) and RPE (MCA2305PE ).

Target Species
Mouse
Product Form
Purified IgG - liquid
Preparation
Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09%Sodium Azide (NaN3)
Immunogen
PU5 1.8 IOE7 Balb/c mouse cell line.
Approx. Protein Concentrations
IgG concentration 1.0mg/ml
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

Pack Size: 0.1 mg
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the product. Storage in frost-free freezers is not recommended.
Pack Size: 0.5 mg
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Instructions For Use
In order to reduce Fc-receptor mediated binding of test antibodies the following procedure is recommended:-

1) Incubate the cell suspension (1 x 106 cells in 100ul) with 1ug of BUF041A (1ul of undiluted reagent, or 10ul of a 1/10 dilution) for 5-10 minutes.

2) Add test antibody according to manufacturers instructions – Do not wash BUF041A off the cells. BUF041A is suitable for use in conjunction with test antibodies from any manufacturer or with in-house antibodies.*

3) Proceed with staining as usual.

* Care is needed in the design of experiments utilizing unconjugated anti-mouse antibodies, to ensure that the secondary antibody being used does not cross-react with SeroBlock FcR. Bio-Rad supply a range of isotype specific anti-mouse immunoglobulin antibodies that may be useful for this purpose.
In order to reduce Fc-receptor mediated binding of test antibodies the following procedure is recommended:-

1) Incubate the cell suspension (1 x 106 cells in 100ul) with 1ug of BUF041B (1ul of undiluted reagent, or 10ul of a 1/10 dilution) for 5-10 minutes.

2) Add test antibody according to manufacturers instructions – Do not wash BUF041B off the cells. BUF041B is suitable for use in conjunction with test antibodies from any manufacturer or with in-house antibodies.*

3) Proceed with staining as usual.

* Care is needed in the design of experiments utilizing unconjugated anti-mouse antibodies, to ensure that the secondary antibody being used does not cross-react with SeroBlock FcR. Bio-Rad supply a range of isotype specific anti-mouse immunoglobulin antibodies that may be useful for this purpose.

References for Mouse Seroblock FcR

  1. Ivanovska, N.D et al . (2008) Properdin Deficiency in Murine Models of Nonseptic Shock.
    The Journal of Immunology. 180: 6962-6969.
  2. Chen, H-F et al . (2009) A reduced oxygen tension (5%) is not beneficial for maintaining human embryonic stem cells in the undifferentiated state with short splitting intervals.
    Human Reproduction. 24: 71-80.
  3. Birjandi, S.Z. et al. (2011) Alterations in marginal zone macrophages and marginal zone B cells in old mice.
    J Immunol. 186: 3441-51.
  4. Cousins, F.L. et al. (2016) Evidence for a dynamic role for mononuclear phagocytes during endometrial repair and remodelling.
    Sci Rep. 6: 36748.
  5. Bi, C.L. et al. (2020) LncRNA NEAT1 promotes malignant phenotypes and TMZ resistance in glioblastoma stem cells by regulating let-7g-5p/MAP3K1 axis.
    Biosci Rep. 40 (10) [Epub ahead of print].
  6. Chiang, M.K. et al. (2021) Two ST11 Klebsiella pneumoniae strains exacerbate colorectal tumorigenesis in a colitis-associated mouse model.
    Gut Microbes. 13 (1): 1980348.
  7. Brulin, B. et al. (2021) Evaluation of the Chemotherapy Drug Response Using Organotypic Cultures of Osteosarcoma Tumours from Mice Models and Canine Patients.
    Cancers (Basel). 13 (19): 4890.
  8. Bonet, A. et al. (2021) Decreased endostatin in db/db retinas is associated with optic disc intravitreal vascularization.
    Exp Eye Res. 212: 108801.
    9. View The Latest Product References
  9. Satofuka, H. et al. (2022) Efficient human-like antibody repertoire and hybridoma production in trans-chromosomic mice carrying megabase-sized human immunoglobulin loci.
    Nat Commun. 13 (1): 1841.

Synonyms
CD16/CD32
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Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"

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