Mouse Seroblock FcR | FCR4G8
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FLA ResetMouse Seroblock FcR
- Product Type
- Accessory Reagent
- Clone
- FCR4G8
- Isotype
- IgG2b
- Specificity
- Mouse Seroblock FcR
Antibody Quality and Performance Guarantee
| Mouse Seroblock FcR is a rat antibody that recognizes mouse CD16 and CD32, cell surface proteins also known as FcRγIII and FcRγII, respectively. The function of these proteins is to bind IgG molecules via their Fc regions as part of the adaptive immune response. CD16 and/or CD32 are expressed by a wide variety of cells, including monocytes, macrophages, B lymphocytes, granulocytes, NK cells, dendritic cells and some activated T lymphocytes. The expression of CD16/CD32 antigens can lead to non-specific binding of test monoclonal antibodies in staining procedures, resulting in “high background” staining on a wide range of cells. This non-specific binding may be blocked by pre-incubation of target cells with BUF041A, resulting in clearer staining. For direct analysis of CD16/32 expression this antibody is also available conjugated directly to FITC (MCA2305F ), AlexaFluor488 (MCA2305A488), AlexaFluor647 (MCA2305A647 ) and RPE (MCA2305PE ). |
- Target Species
- Mouse
- Product Form
- Purified IgG - liquid
- Preparation
- Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
0.09% Sodium Azide (NaN3) - Immunogen
- PU5 1.8 IOE7 Balb/c mouse cell line.
- Approx. Protein Concentrations
- IgG concentration 1.0mg/ml
- Regulatory
- For research purposes only
- Guarantee
- 12 months from date of despatch
Pack Size: 0.1 mg
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the product. Storage in frost-free freezers is not recommended.
Avoid repeated freezing and thawing as this may denature the product. Storage in frost-free freezers is not recommended.
Pack Size: 0.5 mg
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Flow Cytometry |  |
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
- Instructions For Use
- In order to reduce Fc-receptor mediated binding of test antibodies the following procedure is recommended:-
1) Incubate the cell suspension (1 x 106 cells in 100ul) with 1ug of BUF041A (1ul of undiluted reagent, or 10ul of a 1/10 dilution) for 5-10 minutes.
2) Add test antibody according to manufacturers instructions – Do not wash BUF041A off the cells. BUF041A is suitable for use in conjunction with test antibodies from any manufacturer or with in-house antibodies.*
3) Proceed with staining as usual.
* Care is needed in the design of experiments utilizing unconjugated anti-mouse antibodies, to ensure that the secondary antibody being used does not cross-react with SeroBlock FcR. Bio-Rad supply a range of isotype specific anti-mouse immunoglobulin antibodies that may be useful for this purpose. - In order to reduce Fc-receptor mediated binding of test antibodies the following procedure is recommended:-
1) Incubate the cell suspension (1 x 106 cells in 100ul) with 1ug of BUF041B (1ul of undiluted reagent, or 10ul of a 1/10 dilution) for 5-10 minutes.
2) Add test antibody according to manufacturers instructions – Do not wash BUF041B off the cells. BUF041B is suitable for use in conjunction with test antibodies from any manufacturer or with in-house antibodies.*
3) Proceed with staining as usual.
* Care is needed in the design of experiments utilizing unconjugated anti-mouse antibodies, to ensure that the secondary antibody being used does not cross-react with SeroBlock FcR. Bio-Rad supply a range of isotype specific anti-mouse immunoglobulin antibodies that may be useful for this purpose.
References for Mouse Seroblock FcR
-
Ivanovska, N.D. et al. (2008) Properdin deficiency in murine models of nonseptic shock.
J Immunol. 180 (10): 6962-9. -
Chen, H-F et al . (2009) A reduced oxygen tension (5%) is not beneficial for maintaining human embryonic stem cells in the undifferentiated state with short splitting intervals.
Human Reproduction. 24: 71-80. -
Birjandi, S.Z. et al. (2011) Alterations in marginal zone macrophages and marginal zone B cells in old mice.
J Immunol. 186: 3441-51. -
Cousins, F.L. et al. (2016) Evidence for a dynamic role for mononuclear phagocytes during endometrial repair and remodelling.
Sci Rep. 6: 36748. -
Chiang, M.K. et al. (2021) Two ST11 Klebsiella pneumoniae strains exacerbate colorectal tumorigenesis in a colitis-associated mouse model.
Gut Microbes. 13 (1): 1980348. -
Brulin, B. et al. (2021) Evaluation of the Chemotherapy Drug Response Using Organotypic Cultures of Osteosarcoma Tumours from Mice Models and Canine Patients.
Cancers (Basel). 13 (19): 4890. -
Bonet, A. et al. (2021) Decreased endostatin in db/db retinas is associated with optic disc intravitreal vascularization.
Exp Eye Res. 212: 108801. -
Satofuka, H. et al. (2022) Efficient human-like antibody repertoire and hybridoma production in trans-chromosomic mice carrying megabase-sized human immunoglobulin loci.
Nat Commun. 13 (1): 1841.
View The Latest Product References
- Synonyms
- CD16/CD32
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