Mouse Seroblock FcR | FCR4G8

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Mouse Seroblock FcR

Product Type: Accessory Reagent
Clone: FCR4G8
Isotype: IgG2b

Product Code	Applications	Pack Size	Your Price	List Price	Quantity
BUF041A Datasheet	F SDS	0.1 mg	Log in		Get Quote
BUF041B Datasheet	F SDS	0.5 mg	Log in		Get Quote

Mouse Seroblock FcR is a rat antibody that recognises mouse CD16 and CD32, cell surface proteins also known as FcRγIII and FcR&gamma:II, respectively. The function of these proteins is to bind IgG molecules via their Fc regions as part of the adaptive immune response. CD16 and/or CD32 are expressed by a wide variety of cells, including monocytes, macrophages, B lymphocytes, granulocytes, NK cells, dendritic cells and some activated T lymphocytes. The expression of CD16/CD32 antigens can lead to non-specific binding of test monoclonal antibodies in staining procedures, resulting in “high background” staining on a wide range of cells. This non-specific binding may be blocked by pre-incubation of target cells with BUF041A, resulting in clearer staining. For direct analysis of CD16/32 expression this antibody is also available conjugated directly to FITC (MCA2305F ), AlexaFluor488 (MCA2305A488), AlexaFluor647 (MCA2305A647 ) and RPE (MCA2305PE ).

Product Details

Target Species

Mouse

Product Form

Purified IgG - liquid

Preparation

Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant

Buffer Solution

Phosphate buffered saline

Preservative Stabilisers

0.09%

Sodium Azide (NaN₃)

Immunogen

PU5 1.8 IOE7 Balb/c mouse cell line.

Approx. Protein Concentrations

IgG concentration 1.0mg/ml

Storage Information

Storage: Pack Size: 0.1 mg
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the product. Storage in frost-free freezers is not recommended.

Pack Size: 0.5 mg
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
Guarantee: 12 months from date of despatch

More Information

Regulatory: For research purposes only

Applications of Mouse Seroblock FcR

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

Application Name	Verified	Min Dilution	Max Dilution
Flow Cytometry

Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.

Instructions For Use: In order to reduce Fc-receptor mediated binding of test antibodies the following procedure is recommended:-

1) Incubate the cell suspension (1 x 10⁶ cells in 100ul) with 1ug of BUF041A (1ul of undiluted reagent, or 10ul of a 1/10 dilution) for 5-10 minutes.

2) Add test antibody according to manufacturers instructions – Do not wash BUF041A off the cells. BUF041A is suitable for use in conjunction with test antibodies from any manufacturer or with in-house antibodies.*

3) Proceed with staining as usual.

* Care is needed in the design of experiments utilizing unconjugated anti-mouse antibodies, to ensure that the secondary antibody being used does not cross-react with SeroBlock FcR. Bio-Rad supply a range of isotype specific anti-mouse immunoglobulin antibodies that may be useful for this purpose.

Product Specific References

References for Mouse Seroblock FcR

Ivanovska, N.D et al . (2008) Properdin Deficiency in Murine Models of Nonseptic Shock.
The Journal of Immunology. 180: 6962-6969.
Chen, H-F et al . (2009) A reduced oxygen tension (5%) is not beneficial for maintaining human embryonic stem cells in the undifferentiated state with short splitting intervals.
Human Reproduction. 24: 71-80.
Birjandi, S.Z. et al. (2011) Alterations in marginal zone macrophages and marginal zone B cells in old mice.
J Immunol. 186: 3441-51.
Cousins, F.L. et al. (2016) Evidence for a dynamic role for mononuclear phagocytes during endometrial repair and remodelling.
Sci Rep. 6: 36748.
Bi, C.L. et al. (2020) LncRNA NEAT1 promotes malignant phenotypes and TMZ resistance in glioblastoma stem cells by regulating let-7g-5p/MAP3K1 axis.
Biosci Rep. 40 (10) [Epub ahead of print].
Chiang, M.K. et al. (2021) Two ST11 Klebsiella pneumoniae strains exacerbate colorectal tumorigenesis in a colitis-associated mouse model.
Gut Microbes. 13 (1): 1980348.
Brulin, B. et al. (2021) Evaluation of the Chemotherapy Drug Response Using Organotypic Cultures of Osteosarcoma Tumours from Mice Models and Canine Patients.
Cancers (Basel). 13 (19): 4890.
Bonet, A. et al. (2021) Decreased endostatin in db/db retinas is associated with optic disc intravitreal vascularization.
Exp Eye Res. 212: 108801.

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