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Human Seroblock

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Human Seroblock thumbnail image 1 Human Seroblock thumbnail image 2
Human Seroblock gallery image 1
Staining of THP-1 cells with FITC labelled Mouse anti Human CD11a (MCA1848) or Mouse IgG2a Negative Control:FITC (MCA929F) in the absence (A) and presence (B) of Human SeroBlock (BUF070A). Antibody and isotype control were both used neat. Blue shaded areas represents cells stained with the CD11a antibody while the clear areas represents cells stained with isotype control
Human Seroblock gallery image 2
Published customer image:
Human Seroblock (BUF070) used to reduce background staining on human cancer cell lines during TAAR1 analysis by flow cytometry.
Image caption:
TAAR1 expression determined by flow cytometry correlates with intensity of IF staining in a subset of cell lines. a Cells were stained for intracellular TAAR1. The isotype control contained mouse IgG in place of primary antibody to correct for non-specific staining, and was performed on paired samples to those treated with the anti-TAAR1 primary antibody. A mastermix of antibody solutions was prepared for each independent experiment, with each cell line treated simultaneously using the same mastermix. Images are representative samples of 3 independent experiments conducted at different cell passage numbers. b Mean fluorescence intensity for the isotype control and TAAR1 stained cells was determined. Data represent mean ± SEM of 3 independent experiments conducted with different passage numbers of each cell line. Data were compared by two-way ANOVA with Holm–Sidak’s multiple comparison test for individual comparisons where significant main effects were observed. *P <0.05, **P <0.01, ***P <0.001

From: Pitts MS, McShane JN, Hoener MC, Christian SL, Berry MD.
TAAR1 levels and sub-cellular distribution are cell line but not breast cancer subtype specific.
Histochem Cell Biol. 2019 Aug;152(2):155-66.
doi: 10.1007/s00418-019-01791-7.
This image is from an open access article distributed under terms of a Creative Commons Attribution License.

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Human Seroblock

Product Type
Accessory Reagent
Specificity
Human Seroblock

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Product Code Applications Pack Size List Price Your Price Qty
BUF070B
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SDS Safety Datasheet SDS
F 200 Test loader
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loader
BUF070A
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SDS Safety Datasheet SDS
F 50 Test loader
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Human Fc receptors are expressed on a variety of immune cell types including monocytes, macrophages, B cells, granulocytes and dendritic cells. Cells that express Fc receptors can give false positive immunofluorescent staining due to the Fc receptors binding of Ig. Human SeroBlock is designed to prevent such non-specific staining without interfering with appropriate target staining. Human SeroBlock is compatible with use of anti-human antibodies targeting Fc receptors in flow cytometry.

Target Species
Human
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09%Sodium Azide (NaN3)
Regulatory
For research purposes only
Guarantee
Guaranteed until date of expiry. Please see product label.

Store at +4oC. DO NOT FREEZE.
This product should be stored undiluted. Should this product contain a precipitate we recommend microcentrifugation before use.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry *See Instructions For Use
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Instructions For Use
BUF070B, BUF070A
In order to reduce Fc-receptor mediated binding of test antibodies the following procedure is recommended:-

1) Add 5 ul of Human SeroBlock per 100ul cell suspension for 5-10 minutes at room temperature.

2) Add test antibody according to manufacturers instructions – Do not wash Human SeroBlock off the cells. Human SeroBlock is suitable for use in conjunction with test antibodies from any manufacturer or with in-house antibodies.*Human SeroBlock is also compatible with flow cytometric analysis of human Fc receptors.

3) Proceed with staining as usual.

References for Human Seroblock

  1. Boibessot, C. et al. (2021) Using ex vivo. culture to assess dynamic phenotype changes in human prostate macrophages following exposure to therapeutic drugs.
    Sci Rep. 11 (1): 19299.
  2. Boibessot, C. et al. (2022) Subversion of infiltrating prostate macrophages to a mixed immunosuppressive tumor-associated macrophage phenotype.
    Clin Transl Med. 12 (1): e581.
  3. Buchheim, J.I. et al. (2019) Stress Related Shift Toward Inflammaging in Cosmonauts After Long-Duration Space Flight.
    Front Physiol. 10: 85.
  4. Moen, I.N. et al. (2021) Smac-mimetics reduce numbers and viability of human osteoclasts.
    Cell Death Discov. 7 (1): 36.
  5. Munawara, U. et al. (2021) Hyperactivation of monocytes and macrophages in MCI patients contributes to the progression of Alzheimer's disease.
    Immun Ageing. 18 (1): 29.
  6. Pitts, M.S. et al. (2019) TAAR1 levels and sub-cellular distribution are cell line but not breast cancer subtype specific.
    Histochem Cell Biol. 152 (2): 155-166.
  7. Nguyen, V. et al. (2023) An Antibody-Dependent Cellular Cytotoxicity Assay for Detecting Ocrelizumab Neutralizing Antibody.
    AAPS J. 25 (6): 97.
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