BrdU Antibody

BrdU Antibody gallery image 1

HeLa cells were treated with 10 μg BrdU for 1 hour (B) or left untreated (A). Cells were stained with rabbit anti-BrdU antibody (AHP2405) at a dilution of 1/500. As a secondary antibody, sheep anti rabbit IgG DyLight® 549 conjugated antibody (STAR36D549GA) was used at a 1/50 dilution. PureBlu DAPI (1351303) was used as nuclear counterstain.

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BrdU Antibody gallery image 2

BrdU-labeled human lymphoma cells were stained with rabbit anti-BrdU antibody (AHP2405) at a 1/50 dilution (A) and a 1/100 dilution (B). Sheep anti Rabbit IgG DyLight® 488 conjugated antibody (STAR36D488GA) was used as the secondary detection reagent at a 1/50 dilution. READIDROP™ Propidium Iodide (1351101) was used to stain total DNA.

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BrdU Antibody gallery image 3

Immunofluorescence microscopy image of paraformaldehyde-fixed, paraffin-embedded guinea pig pancreas tissue stained with 2.7 μg/ml rabbit anti-BrdU antibody (AHP2405). Donkey anti-rabbit antibody was used as the secondary antibody (red). DAPI was used as nuclear counterstain (blue) and imaged with a 40× objective.

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  • Rabbit anti BrdU
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  • Product Type
    Polyclonal Antibody
  • Isotype
    Polyclonal IgG
1 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    AHP2405F*, IC, IF*, Pdatasheet pdfdatasheet pdf50 µg
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    More Images
    • Rabbit anti BrdU antibody recognizes bromodeoxyuridine (known as BrdU or BrdUrd). BrdU is a synthetic thymidine analog, which when added to cell culture media can be incorporated into the DNA of replicating cells as a substitute for thymidine. This characteristic combined with the ease of detecting incorporated BrdU with the help of anti-BrdU antibodies has resulted in establishing BrdU as a key tool for the visualization of cells in the S-Phase of the cell cycle.

      BrdU is also commonly used in vivo for monitoring cell proliferation in model systems; BrdU can be administered via intraperitoneal injection, subcutaneous osmotic pump implants (Tesfaiqzi et al. 2004) or in drinking water (Moser et al. 2004).

      The cross reactivity of Rabbit anti BrdU antibody to other thymidine analogs, such as 5′-chloro-2′-deoxyuridine (CldU), 5′-iodo-2′-deoxyuridine (IdU) and 2′-deoxy-5-ethynyluridine (EdU) has not been analyzed. However, the antibody does not appear to detect endogenous thymidine.
    • Intended Use
    • Target Species
    • Product Form
      Purified IgG - liquid
    • Reconstitution
    • Preparation
    • Antiserum Preparation
      Antiserum to BrdU was raised by repeated immunisation of rabbits with highly purified antigen. Purified IgG was prepared from whole serum by affinity chromatography.
    • Preservative Stabilisers
      0.09% Sodium Azide (NaN3)
    • Immunogen
      Bromodeoxyuridine conjugated to KLH
    • Purity
    • Approx. Protein Concentrations
      IgG concentration 1.0 mg/ml
    • Reagents In The Kit
    • Preparing The Antibody
    • Test Principle
    • Buffer Solution
      Phosphate buffered saline
    • Storage
      Store at -20oC only.
      Storage in frost-free freezers is not recommended.
      This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
    • Shelf Life
      12 months from date of despatch
    • Acknowledgements
    • Regulatory
      For research purposes only
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution
      Immunohistology - Paraffin1/1001/500
      Flow Cytometry(1)1/501/100
      BrdU labeling and immunostaining protocol can be found at:

    • Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
    • Technical Advice
    • Recommended Protocol
    • ELISA
    • Immunohistology
    • Histology Positive Control Tissue
    • Immunofluorescence
    • Western Blotting
    • Instructions For Use

    Additional BrdU Antibody Formats

    Formats Applications Sizes available
    BrdU Antibody : Purified F*, IC, IF*, P 50 µg
    • Copyright © 2018 Bio-Rad Antibodies (formerly AbD Serotec)

    Recommended Secondary Antibody

      DescriptionProduct CodePack SizeApplicationsList PriceQuantity
      Sheep anti Rabbit IgG:DyLight®488STAR36D488GA0.1 mgF, IF, WB
      Sheep anti Rabbit IgG:DyLight®549STAR36D549GA0.1 mgF, IF

      Recommended Negative Isotype Control

        Useful Reagents

          DescriptionProduct CodePack SizeApplicationsList PriceQuantity
          PUREBLU™ DAPI1351303250 µgF, IF
          READIDROP™ Propidium Iodide13511013 X 3mlF

          Recommended Positive Controls

            Histology Controls

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