We are constantly expanding our range of antibodies and reagents, resources and online tools to help you choose the right antibody, design experiments and achieve more.Sign up now
To prevent non-specific antibody binding to tissue surface structures such as Fcγ receptors, blocking is traditionally performed prior to incubation with the primary antibody (Kim et al. 2016).
Although the impact and merit of this blocking step has recently been questioned by Buchwalow et al. (2011), we recommend including a blocking step in your experimental design.