Tips for Step 13: Washing
Overview
Consider adding detergents such as 0.05% Tween 20 to the wash buffer to reduce high background staining or non-specific antibody binding. However, these detergents may not be compatible with the markings of hydrophobic pens that aid the compartmentalization of liquids on the slide.
Related Resources
- IHC application page
- IHC protocols
- IHC-paraffin tested antibodies
- “Mastering IHC experiments webinar”
- Immunohistochemistry - Paraffin Tips & Tricks
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