Tips for Step 13: Washing
Overview
Consider adding detergents such as 0.05% Tween 20 to the wash buffer to reduce high background staining or non-specific antibody binding. However, these detergents may not be compatible with the markings of hydrophobic pens that aid the compartmentalization of liquids on the slide.
Related Resources
- IHC application page
- IHC protocols
- IHC-paraffin tested antibodies
- “Mastering IHC experiments webinar”
- Immunohistochemistry - Paraffin Tips & Tricks
Support
Be the First to Receive Updates on Products and Resources
Regular communications will include:
- Free resources for your lab including application guides, posters, mini-reviews, protocols, and practical tips
- Notifications about new online tools to support your research, like our spectraviewer tool
- Fab Fragments enewsletter, a monthly snapshot of our latest antibody news
- Flow. Sorted. enewsletter, dedicated to flow cytometry
- RiVETing enewsletter, dedicated news for veterinary researchers
- New product launches, such as our recent launch of TrailBlazer™ Antibodies using unique SpyTag technology
- StarBright™ Dyes – updates on our expanding range of unique proprietary, fluorescent nanoparticles, especially designed for flow cytometry
- Webinar invitations
- Special offers and more
Through the sign-up process, you will have the option to select your areas of interest to make sure you only receive information relevant to you.
You will be able to unsubscribe easily at any time.
