Escherichia coli Antibody

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Escherichia coli Antibody gallery image 1

Published customer image:
AFM scan of E. coli K-12 specifically bound on the cover slip modified by APTES and antibody Bio-Rad 4329-4906. The “Error” signal is shown. (A) Overview and (B) detail of a single cell.

From: Farka, Z et al.Rapid Detection of Microorganisms Based on Active and Passive Modes of QCM. Sensors 2015, 15, 79-92.

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  • Escherichia coli Antibody thumbnail image 1
  • Rabbit anti Escherichia coli:Biotin
  • Rabbit anti Escherichia coli:FITC
  • Rabbit anti Escherichia coli
(Rated 0.0 out of 5 based on 0 customer reviews)
  • Product Type
    Polyclonal Antibody
  • Isotype
    Polyclonal IgG
3 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    4329-4911Edatasheet pdfdatasheet pdf1 ml
    4329-4911
    4329-4916IFdatasheet pdfdatasheet pdf1 ml
    4329-4916
    4329-4906Edatasheet pdfdatasheet pdf1 ml
    4329-4906
    Summary
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    More Images
    References
    Reviews
    -
    • Rabbit anti Escherichia coli antibody recognizes Escherichia coli and is broadly reactive with all somatic and capsular (O and K) antigenic serotypes. The somatic O antigens are composed of lipopolysaccharide complexes which form part of the cell wall structure of E. coli whilst the capsular K antigens are mainly composed of acidic polysaccharide.

      This antibody will remove E.coli proteins from recombinant preparations. Rabbit anti Escherichia coli antibody has not been absorbed and may cross-react with related enterobacteriaceae. Rabbit anti Escherichia coli antibody has been used in ELISA with serotypes O157:H7, O20, O125, 055, 0111 and K12.
    • Intended Use
    • Target Species
      Bacterial
    • Product Form
      Purified IgG conjugated to Biotin - liquid
      Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid
      Purified IgG - liquid
    • Reconstitution
    • Preparation
    • Preservative Stabilisers
      0.1% Sodium Azide (NaN3)
      0.1%Sodium Azide (NaN3)
      1%Bovine Serum Albumin
      0.1%Sodium Azide (NaN3)
    • Immunogen
      A mixture of all antigenic serotypes.
    • Purity
    • Approx. Protein Concentrations
      4.0 mg/ml
      IgG concentration 4.0 mg/ml
      IgG concentration 4.0 mg/ml
    • Reagents In The Kit
    • Preparing The Antibody
    • Test Principle
    • Buffer Solution
      Phosphate buffered saline
      Phosphate buffered saline
      Phosphate buffered saline
    • Storage
      Store at +4oC or at -20oC if preferred.
      Storage in frost-free freezers is not recommended.
      This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
      Store at +4oC or at -20oC if preferred.
      Storage in frost-free freezers is not recommended.
      This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
      Store at +4oC or at -20oC if preferred.
      Storage in frost-free freezers is not recommended.
      This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
    • Shelf Life
      18 months from date of despatch.
      18 months from date of despatch.
      12 months from date of despatch.
    • Acknowledgements
    • Regulatory
      For research purposes only
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution
      ELISA
    • Application NameYesNoMin DilutionMax Dilution
      Immunofluorescence1/101/50
    • Application NameYesNoMin DilutionMax Dilution
      ELISA1/2000

    • Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using the appropriate negative/positive controls.
    • Technical Advice
    • Technical Advice
    • Technical Advice
    • Recommended Protocol
    • Recommended Protocol
    • Recommended Protocol
    • ELISA
    • ELISA
    • ELISA
    • Immunohistology
    • Immunohistology
    • Immunohistology
    • Histology Positive Control Tissue
    • Histology Positive Control Tissue
    • Histology Positive Control Tissue
    • Immunofluorescence
    • Immunofluorescence
    • Immunofluorescence
    • Western Blotting
    • Western Blotting
    • Western Blotting
    • Instructions For Use
    • Instructions For Use
    • Instructions For Use

    Additional Escherichia coli Antibody Formats

    Formats Applications Sizes available
    Escherichia coli Antibody : FITC IF 1 ml
    Escherichia coli Antibody : Purified E 1 ml
    Escherichia coli Antibody : Biotin E 1 ml
    • Copyright © 2017 Bio-Rad Antibodies (formerly AbD Serotec)

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      Recommended Negative Isotype Control

        Useful Reagents

          Recommended Positive Controls

            Histology Controls

              References

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