Borrelia burgdorferi Antibody

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Published customer image:
Rabbit anti Borrelia burgdorferi antibody used for the detection of B. burgdorferi in canine myocardium by immunohistochemistry on formalin fixed, paraffin embedded tissue sections.
Image caption:
Microscopic examination of heart specimens. A- degeneration of myocardium with disturbances in cardiomyocyte structure, presence of fibrous tissue and mild inflammatory infiltrates (arrows) (H&E; 200x); B-severe cardiomyocyte degeneration accompanied by severe granulocytic and slight lymph o-plasmocytic infiltration in heart tissue adjutant to embolic material (H&E; 200x); C-severe granulocytic and moderate lympho-plasmocytic infiltration of infarct site (H&E; 200x); D-vegetative and spore forms of Borrelia burgdorferi in heart specimens (IHC stain, 600x); E-Borrelia burgdorferi spore forms in heart specimens (IHC stain, 600x).

From: Janus, I. et al. Myocarditis in dogs: etiology, clinical and histopathological features. 11 cases (2007-2013). Irish Vet J. 2010 67:28.

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  • Rabbit anti Borrelia burgdorferi:FITC
  • Rabbit anti Borrelia burgdorferi
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  • Product Type
    Polyclonal Antibody
  • Isotype
    Polyclonal IgG
2 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    1439-9446IF*datasheet pdfdatasheet pdf1 ml
    1439-9446
    1439-9406C, IF, P, WBdatasheet pdfdatasheet pdf1 ml
    1439-9406
    Summary
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    More Images
    References
    Reviews
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    • Rabbit anti Borrelia burgdorferi recognizes one of the causative agents of Lyme disease. B. burgdorferi is a spirochaete bacterium and one of the few pathogenic bacteria capable of surviving in the absence of iron.

      This antibody cross reacts with Treponema pallidum, Borrelia hermsii and Borrelia parkeri.
    • Intended Use
    • Target Species
      Bacterial
    • Product Form
      Purified Ig conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid
      Purified IgG - liquid
    • Reconstitution
    • Preparation
    • Preservative Stabilisers
      0.1% Sodium Azide (NaN3)
      1% Bovine Serum Albumin
      0.1% Sodium Azide (NaN3)
    • Immunogen
      Whole cell preparation from B. burgdorferi.
    • Purity
    • Approx. Protein Concentrations
      IgG concentration 4 mg/ml
      4 mg/ml
    • Reagents In The Kit
    • Preparing The Antibody
    • Test Principle
    • Buffer Solution
      Phosphate buffered saline
      Phosphate buffered saline
    • Storage
      Store at +4oC or at -20oC if preferred.
      Storage in frost-free freezers is not recommended.
      This product should be stored undiluted.
      This product is photosensitive and should be protected from light.
      Avoid repeated freezing and thawing as this may denature the antibody.
      Should this product contain a precipitate we recommend microcentrifugation before use.
      Store at +4oC or at -20oC if preferred.
      Storage in frost-free freezers is not recommended.
      This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody.
    • Shelf Life
      18 months from date of despatch.
      18 months from date of despatch.
    • Acknowledgements
    • Regulatory
      For research purposes only
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution
      Immunofluorescence(1)1/101/50
      (1)
      Acetone fixation is recommended for best results.
    • Application NameYesNoMin DilutionMax Dilution
      Immunofluorescence
      Immunohistology - Frozen
      Immunohistology - Paraffin
      Western Blotting

    • Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using the appropriate negative/positive controls.
    • Technical Advice
    • Technical Advice
    • Recommended Protocol
    • Recommended Protocol
    • ELISA
    • ELISA
    • Immunohistology
    • Immunohistology
    • Histology Positive Control Tissue
    • Histology Positive Control Tissue
    • Immunofluorescence
    • Immunofluorescence
    • Western Blotting
    • Western Blotting
    • Instructions For Use
    • Instructions For Use

    Additional Borrelia burgdorferi Antibody Formats

    Formats Applications Sizes available
    Borrelia burgdorferi Antibody : FITC IF* 1 ml
    Borrelia burgdorferi Antibody : Purified C, IF, P, WB 1 ml
    • Copyright © 2016 Bio-Rad

    Recommended Secondary Antibody

      DescriptionProduct CodePack SizeApplicationsList PriceQuantity
      Goat anti Rabbit IgG (Fc):BiotinSTAR121B1 mgE, WB
      STAR121B
      Sheep anti Rabbit IgG:Biotin2AB02B1 mlC, E, P, WB
      2AB02B
      Sheep anti Rabbit IgG:DyLight®488STAR36D488GA0.1 mgF, IF
      STAR36D488GA
      Sheep anti Rabbit IgG:DyLight®549STAR36D549GA0.1 mgF, IF
      STAR36D549GA
      Sheep anti Rabbit IgG:DyLight®649STAR36D649GA0.1 mgF, IF
      STAR36D649GA
      Sheep anti Rabbit IgG:Dylight®800STAR36D800GA0.1 mgF, IF, WB
      STAR36D800GA
      Goat anti Rabbit IgG (Fc):FITCSTAR121F1 mgF
      STAR121F
      Sheep anti Rabbit IgG:FITCSTAR34B1 mgC, F
      STAR34B
      Goat anti Rabbit IgG (Fc):HRPSTAR121P1 mgE, WB
      STAR121P
      Goat anti Rabbit IgG (H/L):HRPSTAR124P1 mgC, E, WB
      STAR124P
      Sheep anti Rabbit IgG:HRP (Multi Adsorbed)STAR541 mgC, E, WB
      STAR54
      Sheep anti Rabbit IgG:RPESTAR35A1 mlF
      STAR35A

      Recommended Negative Isotype Control

        Useful Reagents

          DescriptionProduct CodePack SizeApplicationsList PriceQuantity
          Antigen Retrieval Buffer, pH8.0BUF025C50 mlP
          BUF025C
          TidyBlot Western Blot Detection Reagent:HRPSTAR209P0.5 mlWB*
          STAR209P
          Antigen Retrieval Buffer, pH8.0BUF025A500 mlP
          BUF025A

          Recommended Positive Controls

            Histology Controls

              References

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